Reassessing the polyphase Neoproterozoic evolution of the Punta del Este Terrane, Dom Feliciano Belt, Uruguay

Some recent models challenge the position and extension of the assumed oceanic basins formed through the break-up of Rodinia, and the tectonic processes involved in the Gondwana assembly, making the investigation of the Early Neoproterozoic record of great relevance. Within the South-American Atlantic margin, the Punta del Este Terrane (PET) of the Dom Feliciano Belt (DFB) comprises a unique Tonian to Ediacaran record, and has a strategic position to reconstruct spatio-temporal relationships with the southern African orogenic belts. Novel zircon U–Pb and Lu–Hf data from the PET basement orthogneisses display Tonian magmatic ages (805–760 Ma) and Hf isotopic signatures indicative of mainly crustal/metasedimentary sources, (Nd TDM ages: 2.2–1.9 Ga, and εHf(t): − 12 to − 4). The basement paragneisses yielded late Paleoproterozoic to Neoproterozoic U–Pb ages, but dominantly positive εHf(t) values. The presented results confirm the correlation of the PET with the Coastal Terrane of the Kaoko Belt, and discard the idea of the Nico Pérez Terrane as a source. Detrital zircon U–Pb and Lu–Hf data from the Rocha Formation yielded a main peak at ca. 660 Ma, with the Neoproterozoic grains showing a εHf(t) between + 1 and + 14. The deposition age of the Rocha Formation is constrained by the youngest detrital zircon age peak (660 Ma), and the beginning of the deposition of the Sierra de Aguirre Formation (580 Ma). The data indicate common sources with the Marmora Terrane, and it is thus proposed that the Rocha Formation belongs to the Gariep Belt, and it was juxtaposed during the Ediacaran to the DFB.Fil: Silva Lara, Hernan. Universität Göttingen; AlemaniaFil: Siegesmund, S.. Universität Göttingen; AlemaniaFil: Oriolo, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geociencias Básicas, Aplicadas y Ambientales de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geociencias Básicas, Aplicadas y Ambientales de Buenos Aires; ArgentinaFil: Hueck, M.. Universidade de Sao Paulo; Brasil. Universität Göttingen; AlemaniaFil: Wemmer, K.. Universität Göttingen; AlemaniaFil: Basei, M. A. S.. Universidade de Sao Paulo; BrasilFil: Oyhantçabal, P.. Universidad de la República; Urugua

Salmonella Pathogenesis and Processing of Secreted Effectors by Caspase-3

The enteric pathogen Salmonella enterica serovar Typhimurium causes food poisoning resulting in gastroenteritis. The S. Typhimurium effector Salmonella invasion protein A (SipA) promotes gastroenteritis by functional motifs that trigger either mechanisms of inflammation or bacterial entry. During infection of intestinal epithelial cells, SipA was found to be responsible for the early activation of caspase-3, an enzyme that is required for SipA cleavage at a specific recognition motif that divided the protein into its two functional domains and activated SipA in a manner necessary for pathogenicity. Other caspase-3 cleavage sites identified in S. Typhimurium appeared to be restricted to secreted effector proteins, which indicates that this may be a general strategy used by this pathogen for processing of its secreted effectors

Reassessing the polyphase neoproterozoic evolution of the Punta del Este terrane, Dom Feliciano Belt, Uruguay

Some recent models challenge the position and extension of the assumed oceanic basins formed through the break-up of Rodinia, and the tectonic processes involved in the Gondwana assembly, making the investigation of the Early Neoproterozoic record of great relevance. Within the South-American Atlantic margin, the Punta del Este Terrane (PET) of the Dom Feliciano Belt (DFB) comprises a unique Tonian to Ediacaran record, and has a strategic position to reconstruct spatio-temporal relationships with the southern African orogenic belts. Novel zircon U–Pb and Lu–Hf data from the PET basement orthogneisses display Tonian magmatic ages (805–760 Ma) and Hf isotopic signatures indicative of mainly crustal/metasedimentary sources, (Nd TDM ages: 2.2–1.9 Ga, and εHf(t): −12 to −4). The basement paragneisses yielded late Paleoproterozoic to Neoproterozoic U–Pb ages, but dominantly positive εHf(t) values. The presented results confrm the correlation of the PET with the Coastal Terrane of the Kaoko Belt, and discard the idea of the Nico Pérez Terrane as a source. Detrital zircon U–Pb and Lu–Hf data from the Rocha Formation yielded a main peak at ca. 660 Ma, with the Neoproterozoic grains showing a εHf(t) between+1 and+14. The deposition age of the Rocha Formation is constrained by the youngest detrital zircon age peak (660 Ma), and the beginning of the deposition of the Sierra de Aguirre Formation (580 Ma). The data indicate common sources with the Marmora Terrane, and it is thus proposed that the Rocha Formation belongs to the Gariep Belt, and it was juxtaposed during the Ediacaran to the DFB

From DNA sequence to application: possibilities and complications

The development of sophisticated genetic tools during the past 15 years have facilitated a tremendous increase of fundamental and application-oriented knowledge of lactic acid bacteria (LAB) and their bacteriophages. This knowledge relates both to the assignments of open reading frames (ORF’s) and the function of non-coding DNA sequences. Comparison of the complete nucleotide sequences of several LAB bacteriophages has revealed that their chromosomes have a fixed, modular structure, each module having a set of genes involved in a specific phase of the bacteriophage life cycle. LAB bacteriophage genes and DNA sequences have been used for the construction of temperature-inducible gene expression systems, gene-integration systems, and bacteriophage defence systems. The function of several LAB open reading frames and transcriptional units have been identified and characterized in detail. Many of these could find practical applications, such as induced lysis of LAB to enhance cheese ripening and re-routing of carbon fluxes for the production of a specific amino acid enantiomer. More knowledge has also become available concerning the function and structure of non-coding DNA positioned at or in the vicinity of promoters. In several cases the mRNA produced from this DNA contains a transcriptional terminator-antiterminator pair, in which the antiterminator can be stabilized either by uncharged tRNA or by interaction with a regulatory protein, thus preventing formation of the terminator so that mRNA elongation can proceed. Evidence has accumulated showing that also in LAB carbon catabolite repression in LAB is mediated by specific DNA elements in the vicinity of promoters governing the transcription of catabolic operons. Although some biological barriers have yet to be solved, the vast body of scientific information presently available allows the construction of tailor-made genetically modified LAB. Today, it appears that societal constraints rather than biological hurdles impede the use of genetically modified LAB.

Presence of genes for type III secretion system 2 in Vibrio mimicus strains

<p>Abstract</p> <p>Background</p> <p>Vibrios, which include more than 100 species, are ubiquitous in marine and estuarine environments, and several of them e.g. <it>Vibrio cholerae</it>, <it>V. parahaemolyticus</it>, <it>V. vulnificus </it>and <it>V. mimicus</it>, are pathogens for humans. Pathogenic <it>V. parahaemolyticus </it>strains possess two sets of genes for type III secretion system (T3SS), T3SS1 and T3SS2. The latter are critical for virulence of the organism and be classified into two distinct phylogroups, T3SS2α and T3SS2β, which are reportedly also found in pathogenic <it>V. cholerae </it>non-O1/non-O139 serogroup strains. However, whether T3SS2-related genes are present in other <it>Vibrio </it>species remains unclear.</p> <p>Results</p> <p>We therefore examined the distribution of the genes for T3SS2 in vibrios other than <it>V. parahaemolyticus </it>by using a PCR assay targeting both T3SS2α and T3SS2β genes. Among the 32 <it>Vibrio </it>species tested in our study, several T3SS2-related genes were detected in three species, <it>V. cholerae</it>, <it>V. mimicus </it>and <it>V. hollisae</it>, and most of the essential genes for type III secretion were present in T3SS2-positive <it>V. cholerae </it>and <it>V. mimicus </it>strains. Moreover, both <it>V. mimicus </it>strains possessing T3SS2α and T3SS2β were identified. The gene organization of the T3SS2 gene clusters in <it>V. mimicus </it>strains was fundamentally similar to that of <it>V. parahaemolyticus </it>and <it>V. cholerae </it>in both T3SS2α- and T3SS2β-possessing strains.</p> <p>Conclusions</p> <p>This study is the first reported evidence of the presence of T3SS2 gene clusters in <it>V. mimicus </it>strains. This finding thus provides a new insight into the pathogenicity of the <it>V. mimicus </it>species.</p

Soybean Seed Extracts Preferentially Express Genomic Loci of Bradyrhizobium japonicum in the Initial Interaction with Soybean, Glycine max (L.) Merr

Initial interaction between rhizobia and legumes actually starts via encounters of both partners in the rhizosphere. In this study, the global expression profiles of Bradyrhizobium japonicum USDA 110 in response to soybean (Glycine max) seed extracts (SSE) and genistein, a major soybean-released isoflavone for nod genes induction of B. japonicum, were compared. SSE induced many genomic loci as compared with genistein (5.0 µM), nevertheless SSE-supplemented medium contained 4.7 µM genistein. SSE markedly induced four predominant genomic regions within a large symbiosis island (681 kb), which include tts genes (type III secretion system) and various nod genes. In addition, SSE-treated cells expressed many genomic loci containing genes for polygalacturonase (cell-wall degradation), exopolysaccharide synthesis, 1-aminocyclopropane-1-carboxylate deaminase, ribosome proteins family and energy metabolism even outside symbiosis island. On the other hand, genistein-treated cells exclusively showed one expression cluster including common nod gene operon within symbiosis island and six expression loci including multidrug resistance, which were shared with SSE-treated cells. Twelve putatively regulated genes were indeed validated by quantitative RT-PCR. Several SSE-induced genomic loci likely participate in the initial interaction with legumes. Thus, these results can provide a basic knowledge for screening novel genes relevant to the B. japonicum- soybean symbiosis

Genetic Characterization of Conserved Charged Residues in the Bacterial Flagellar Type III Export Protein FlhA

For assembly of the bacterial flagellum, most of flagellar proteins are transported to the distal end of the flagellum by the flagellar type III protein export apparatus powered by proton motive force (PMF) across the cytoplasmic membrane. FlhA is an integral membrane protein of the export apparatus and is involved in an early stage of the export process along with three soluble proteins, FliH, FliI, and FliJ, but the energy coupling mechanism remains unknown. Here, we carried out site-directed mutagenesis of eight, highly conserved charged residues in putative juxta- and trans-membrane helices of FlhA. Only Asp-208 was an essential acidic residue. Most of the FlhA substitutions were tolerated, but resulted in loss-of-function in the ΔfliH-fliI mutant background, even with the second-site flhB(P28T) mutation that increases the probability of flagellar protein export in the absence of FliH and FliI. The addition of FliH and FliI allowed the D45A, R85A, R94K and R270A mutant proteins to work even in the presence of the flhB(P28T) mutation. Suppressor analysis of a flhA(K203W) mutation showed an interaction between FlhA and FliR. Taken all together, we suggest that Asp-208 is directly involved in PMF-driven protein export and that the cooperative interactions of FlhA with FlhB, FliH, FliI, and FliR drive the translocation of export substrate