Accommodation to hyperpolarization of human axons assessed in the frequency domain

Human axonsin vivowere subjected to subthreshold currents with a threshold-"ZAP" profile (Impedance [ Z: ] A: mplitude P: rofile) to allow the use of frequency domain techniques to determine the propensity for resonant behavior, and to clarify the relative contributions of different ion channels to their low-frequency responsiveness. Twenty-four studies were performed on the motor and sensory axons in 6 subjects. The response to oscillatory currents was tested between 'DC' and 16 Hz. A resonant peak at ~2 to 2.5 Hz was found in the response of hyperpolarized axons, but there was only a small broad response in axons at resting membrane potential (RMP). A mathematical model of axonal excitability developed using DC pulses provided a good fit to the frequency response for human axons, and indicated that the hyperpolarization-activated currentIh, and the slow potassium currentIKsare principally responsible for the resonance. However the results indicate that if axons are hyperpolarized more than -60% of resting threshold, the only conductances that are appreciably active areIhand the leak conductance - i.e., that the activity of these conductances can be studiedin vivovirtually in isolation at hyperpolarized membrane potentials. Given that the leak conductance dampens resonance it is suggested that the -60% hyperpolarization used here is optimal forIh As expected differences between the frequency responses of motor and sensory axons were present and best explained by reduced GKs, up-modulation ofIhand increased persistent Na(+)current,INaP(due to depolarization of RMP) in sensory axons

Transport into the troposphere in a tropopause fold/cut-off low system

A tropopause fold developed on the western flank of a trough in the 300 mb flow on 6 Oct. 1990. Radiosonde ascents over western Europe showed very dry stable layers beneath the jet stream in the potential temperature range 310 to 315 K. These were evident on profiles from 12h on 6 Oct. to 00h on 8 Oct. ECMWF model assimilations were examined for this period to determine how well the model represented the radiosonde observations. Humidity fields were found to give better agreement than potential vorticity, probably because the PV is affected by the limited vertical resolution of the model. Isentropic trajectories were calculated for the air in the fold as represented by the ECMWF assimilation at 00h on 7 Oct. Those on the western edge of the fold split from the main flow and transferred to the troposphere, while those on the eastern side ended up in the cut-off low. A lower bound of 1.1 x 10(exp 14) kg is estimated for the amount of stratospheric air transferred into the troposphere by this fold

Connection between total ozone fields and lower stratospheric dynamics

It is expected from theoretical considerations that synoptic-scale variations in total ozone should be correlated with the absolute vorticity field near the tropopause. This paper tests the theory, using TOMS total ozone fields and vorticity derived from ECMWF analyses. A good correlation is found, except during winter - suggesting that other sources of variability in total ozone are active at this time. The correlation with potential vorticity is also investigated. This shows two maxima in the correlation coefficient in winter and spring, one near the tropopause and the other in the region of 420K. A study of the residuals from a linear regression of vorticity with total ozone during January reveals a similar structure to the 450K potential vorticity field

Three-dimensional coherent X-ray diffraction imaging of a ceramic nanofoam: determination of structural deformation mechanisms

Ultra-low density polymers, metals, and ceramic nanofoams are valued for their high strength-to-weight ratio, high surface area and insulating properties ascribed to their structural geometry. We obtain the labrynthine internal structure of a tantalum oxide nanofoam by X-ray diffractive imaging. Finite element analysis from the structure reveals mechanical properties consistent with bulk samples and with a diffusion limited cluster aggregation model, while excess mass on the nodes discounts the dangling fragments hypothesis of percolation theory.Comment: 8 pages, 5 figures, 30 reference

Dose, exposure time, and resolution in Serial X-ray Crystallography

The resolution of X-ray diffraction microscopy is limited by the maximum dose that can be delivered prior to sample damage. In the proposed Serial Crystallography method, the damage problem is addressed by distributing the total dose over many identical hydrated macromolecules running continuously in a single-file train across a continuous X-ray beam, and resolution is then limited only by the available molecular and X-ray fluxes and molecular alignment. Orientation of the diffracting molecules is achieved by laser alignment. We evaluate the incident X-ray fluence (energy/area) required to obtain a given resolution from (1) an analytical model, giving the count rate at the maximum scattering angle for a model protein, (2) explicit simulation of diffraction patterns for a GroEL-GroES protein complex, and (3) the frequency cut off of the transfer function following iterative solution of the phase problem, and reconstruction of an electron density map in the projection approximation. These calculations include counting shot noise and multiple starts of the phasing algorithm. The results indicate counting time and the number of proteins needed within the beam at any instant for a given resolution and X-ray flux. We confirm an inverse fourth power dependence of exposure time on resolution, with important implications for all coherent X-ray imaging. We find that multiple single-file protein beams will be needed for sub-nanometer resolution on current third generation synchrotrons, but not on fourth generation designs, where reconstruction of secondary protein structure at a resolution of 0.7 nm should be possible with short exposures.Comment: 19 pages, 7 figures, 1 tabl

BODIPY-based conjugated polymers for broadband light sensing and harvesting applications

The synthesis of novel low band-gap polymers has significantly improved light sensing and harvesting in polymer-fullerene devices. Here the synthesis of two low band-gap polymers based on the 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene core (BODIPY), and either bis(3,4-ethylenedioxythiophene) (bis-EDOT) or its all-sulfur analogue bis(3,4-ethylenedithiathiophene) (bis-EDTT) are described. The polymers demonstrate ambipolar charge transport and are shown to be suitable for broadband light sensing and solar energy harvesting in solution-processable polymer-fullerene devices

To be or not to be an auctioneer: Some thoughts on the legal nature of online eBay auctions and the protection of consumers

This paper discusses the legal classification of online “eBay” auctions. The discussion has key implications on the scope of consumer protection law as sale by auctions are, for example, excluded from the scope of the Consumer Protection (Distance Selling) Regulations 2000. The paper uncovers that online “eBay” auctions cannot always be considered as traditional auctions and that eBay, as an intermediary, is not to be considered as an auctioneer. This creates difficulties associated with a distributive application of consumer protection laws such as the Consumer Protection (Distance Selling) Regulations 2000. Another set of difficulties is associated with a lenient legal regime applicable to the liability of eBay under the Electronic Commerce (EC Directive) Regulations 2002 . The paper concludes that there is an urgent need to clarify the legal classification of online auctions and to rethink the liability of online auction sites to better protect consumers

Consumer credit information systems: A critical review of the literature. Too little attention paid by lawyers?

This paper reviews the existing literature on consumer credit reporting, the most extensively used instrument to overcome information asymmetry and adverse selection problems in credit markets. Despite the copious literature in economics and some research in regulatory policy, the legal community has paid almost no attention to the legal framework of consumer credit information systems, especially within the context of the European Union. Studies on the topic, however, seem particularly relevant in view of the establishment of a single market for consumer credit. This article ultimately calls for further legal research to address consumer protection concerns and inform future legislation

Recent advances in understanding the effects of climate change on coral reefs

Climate change is one of the greatest threats to the persistence of coral reefs. Sustained and ongoing increases in ocean temperatures and acidification are altering the structure and function of reefs globally. Here, we summarise recent advances in our understanding of the effects of climate change on scleractinian corals and reef fish. Although there is considerable among-species variability in responses to increasing temperature and seawater chemistry, changing temperature regimes are likely to have the greatest influence on the structure of coral and fish assemblages, at least over short–medium timeframes. Recent evidence of increases in coral bleaching thresholds, local genetic adaptation and inheritance of heat tolerance suggest that coral populations may have some capacity to respond to warming, although the extent to which these changes can keep pace with changing environmental conditions is unknown. For coral reef fishes, current evidence indicates increasing seawater temperature will be a major determinant of future assemblages, through both habitat degradation and direct effects on physiology and behaviour. The effects of climate change are, however, being compounded by a range of anthropogenic disturbances, which may undermine the capacity of coral reef organisms to acclimate and/or adapt to specific changes in environmental conditions