931 research outputs found
ENERGY TRANSFER IN TRIMERIC C-PHYCOCYANIN STUDIED BY PICOSECOND FLUORESCENCE KINETICS
The excited state kinetics of trimeric C-phycocyanin from Mastigocladus laminosus has been measured as a function of the emission and excitation wavelength by the single-photon timing technique with picosecond resolution and simultaneous data analysis. A fast decay component of 22 ps (C-phycocyanin with linker peptides) and 36 ps (C-phycocyanin lacking linker peptides) is attributed to efficient energy transfer from sensitizing to fluorescing chromophores. At long detection wavelengths the fast decay components are found to turn into a rise term. This finding further corroborates the concept of intramolecular energy transfer. Previous reports on the conformational heterogeneity of the chromophores and/or proteins in C-phycocyanin are confirmed. Our data also provide indications for the importance of the uncoloured linker peptides for this heterogeneity
Optical frequency comb generation from a monolithic microresonator
Optical frequency combs provide equidistant frequency markers in the
infrared, visible and ultra-violet and can link an unknown optical frequency to
a radio or microwave frequency reference. Since their inception frequency combs
have triggered major advances in optical frequency metrology and precision
measurements and in applications such as broadband laser-based gas sensing8 and
molecular fingerprinting. Early work generated frequency combs by intra-cavity
phase modulation while to date frequency combs are generated utilizing the
comb-like mode structure of mode-locked lasers, whose repetition rate and
carrier envelope phase can be stabilized. Here, we report an entirely novel
approach in which equally spaced frequency markers are generated from a
continuous wave (CW) pump laser of a known frequency interacting with the modes
of a monolithic high-Q microresonator13 via the Kerr nonlinearity. The
intrinsically broadband nature of parametric gain enables the generation of
discrete comb modes over a 500 nm wide span (ca. 70 THz) around 1550 nm without
relying on any external spectral broadening. Optical-heterodyne-based
measurements reveal that cascaded parametric interactions give rise to an
optical frequency comb, overcoming passive cavity dispersion. The uniformity of
the mode spacing has been verified to within a relative experimental precision
of 7.3*10(-18).Comment: Manuscript and Supplementary Informatio
FĂRSTER TRANSFER CALCULATIONS BASED ON CRYSTAL STRUCTURE DATA FROM Agmenellum quadruplicatum C-PHYCOCYANIN
Excitation energy transfer in C-phycocyanin is modeled using the Forster inductive resonance mechanism. Detailed calculations are carried out using coordinates and orientations of the chromophores derived from X-ray crystallographic studies of C-phycocyanin from two different species (Schirmer et al, J. Mol. Biol. 184, 257â277 (1985) and ibid., 188, 651-677 (1986)). Spectral overlap integrals are estimated from absorption and fluorescence spectra of C-phycocyanin of Mastigocladus laminosus and its separated subunits. Calculations are carried out for the ÎČ-subunit, αÎČ-monomer, (αÎČ)3-trimer and (αÎČ)0-hexamer species with the following chromophore assignments: ÎČ155 = 'sâ(sensitizer), ÎČ84 =âf (fluorescer) and α84 =âmâ(intermediate):]:. The calculations show that excitation transfer relaxation occurs to 3=98% within 200 ps in nearly every case; however, the rates increase as much as 10-fold for the higher aggregates. Comparison with experimental data on fluorescence decay and depolarization kinetics from the literature shows qualitative agreement with these calculations. We conclude that Forster transfer is sufficient to account for all of the observed fluorescence properties of C-phycocyanin in aggregation states up to the hexamer and in the absence of linker polypeptides
Comb-calibrated solar spectroscopy through a multiplexed single-mode fiber channel
We investigate a new scheme for astronomical spectrograph calibration using
the laser frequency comb at the Solar Vacuum Tower Telescope on Tenerife. Our
concept is based upon a single-mode fiber channel, that simultaneously feeds
the spectrograph with comb light and sunlight. This yields nearly perfect
spatial mode matching between the two sources. In combination with the absolute
calibration provided by the frequency comb, this method enables extremely
robust and accurate spectroscopic measurements. The performance of this scheme
is compared to a sequence of alternating comb and sunlight, and to absorption
lines from Earth's atmosphere. We also show how the method can be used for
radial-velocity detection by measuring the well-explored 5-minute oscillations
averaged over the full solar disk. Our method is currently restricted to solar
spectroscopy, but with further evolving fiber-injection techniques it could
become an option even for faint astronomical targets.Comment: 21 pages, 11 figures. A video abstract for this paper is available on
youtube. For watching the video, please follow
https://www.youtube.com/watch?v=oshdZgrt89I . The video abstract is also
available for streaming and download on the related article website of New
Journal of Physic
On the quantization of SU(3)-skyrmions
The quantization condition derived previously for SU(2) solitons quantized
with SU(3)-collective coordinates is generalized for SU(3) skyrmions with
strangeness content different from zero. Quantization of the dipole-type
configuration with large strangeness content found recently is considered as an
example.Comment: 7 pages, 2 figures (available by request
BILIPROTEINS FROM THE BUTTERFLY Pieris brassicae STUDIED BY TIME-RESOLVED FLUORESCENCE AND COHERENT ANTI-STOKES RAMAN SPECTROSCOPY
The fluorescence decay time of the biliverdin IX7 chromophore present in biliproteins isolated from Pieris brassicae is determined to be 44 ± 3 ps. This value suggests a cyclic helical chromophore structure. The vibrational frequencies determined by CARS-spectroscopy are compared with those of model compounds. The data confirm that the chromophore in the protein-bound state adopts a cyclic-helical, flexible conformation
Precision spectroscopy of the 3s-3p fine structure doublet in Mg+
We apply a recently demonstrated method for precision spectroscopy on strong
transitions in trapped ions to measure both fine structure components of the
3s-3p transition in 24-Mg+ and 26-Mg+. We deduce absolute frequency reference
data for transition frequencies, isotope shifts and fine structure splittings
that are in particular useful for comparison with quasar absorption spectra,
which test possible space-time variations of the fine structure constant. The
measurement accuracy improves previous literature values, when existing, by
more than two orders of magnitude
Full Stabilization of a Microresonator based Optical Frequency Comb
We demonstrate control and stabilization of an optical frequency comb
generated by four-wave mixing in a monolithic microresonator with a mode
spacing in the microwave regime (86 GHz). The comb parameters (mode spacing and
offset frequency) are controlled via the power and the frequency of the pump
laser, which constitutes one of the comb modes. Furthermore, generation of a
microwave beat note at the comb's mode spacing frequency is presented, enabling
direct stabilization to a microwave frequency standard
A Frequency Comb calibrated Solar Atlas
The solar spectrum is a primary reference for the study of physical processes
in stars and their variation during activity cycles. In Nov 2010 an experiment
with a prototype of a Laser Frequency Comb (LFC) calibration system was
performed with the HARPS spectrograph of the 3.6m ESO telescope at La Silla
during which high signal-to-noise spectra of the Moon were obtained. We exploit
those Echelle spectra to study the optical integrated solar spectrum . The
DAOSPEC program is used to measure solar line positions through gaussian
fitting in an automatic way. We first apply the LFC solar spectrum to
characterize the CCDs of the HARPS spectrograph. The comparison of the LFC and
Th-Ar calibrated spectra reveals S-type distortions on each order along the
whole spectral range with an amplitude of +/-40 m/s. This confirms the pattern
found by Wilken et al. (2010) on a single order and extends the detection of
the distortions to the whole analyzed region revealing that the precise shape
varies with wavelength. A new data reduction is implemented to deal with CCD
pixel inequalities to obtain a wavelength corrected solar spectrum. By using
this spectrum we provide a new LFC calibrated solar atlas with 400 line
positions in the range of 476-530, and 175 lines in the 534-585 nm range. The
new LFC atlas improves the accuracy of individual lines by a significant factor
reaching a mean value of about 10 m/s. The LFC--based solar line wavelengths
are essentially free of major instrumental effects and provide a reference for
absolute solar line positions. We suggest that future LFC observations could be
used to trace small radial velocity changes of the whole solar photospheric
spectrum in connection with the solar cycle and for direct comparison with the
predicted line positions of 3D radiative hydrodynamical models of the solar
photosphere.Comment: Accept on the 15th of October 2013. 9 pages, 10 figures. ON-lINE data
A&A 201
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