DC and low-frequency noise analysis for buried SiGe channel metamorphic PMOSFETs with high Ge content, Journal of Telecommunications and Information Technology, 2005, nr 1

Measurements of current drive in p-Si1-xGex MOSFETs, with x = 0.7, 0.8 reveal an enhancement ratio of over 2 times as compared to a Si device at an effective channel length of 0.55 um. They also show a lower knee voltage in the output I-V characteristics while retaining similar values of drain induced barrier lowering, subthreshold swing, and off current for devices with a Sb punch-through stopper. For the first time, we have quantitatively explained the low-frequency noise reduction in metamorphic, high Ge content, SiGe PMOSFETs compared to Si PMOSFETs

Vitamin D3 Deficiency Differentially Affects Functional and Disease Outcomes in the G93A Mouse Model of Amyotrophic Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) is a neuromuscular disease characterized by motor neuron death in the central nervous system. Vitamin D supplementation increases antioxidant activity, reduces inflammation and improves motor neuron survival. We have previously demonstrated that vitamin D3 supplementation at 10× the adequate intake improves functional outcomes in a mouse model of ALS

DC and low-frequency noise analysis for buried SiGe channel metamorphic PMOSFETs with high Ge content

Measurements of current drive in p-Si1-xGex MOSFETs, with x = 0.7, 0.8 reveal an enhancement ratio of over 2 times as compared to a Si device at an effective channel length of 0.55 žm. They also show a lower knee voltage in the output I-V characteristics while retaining similar values of drain induced barrier lowering, subthreshold swing, and off current for devices with a Sb punch-through stopper. For the first time, we have quantitatively explained the low-frequency noise reduction in metamorphic, high Ge content, SiGe PMOSFETs compared to Si PMOSFETs

Cytokine profiles of primDCs stimulated with LPS-depleted allergen + LPS.

<p>Profile of cytokines released by primary dendritic cells after 24 hours of stimulation with different concentrations of LPS (0.1, 1 or 10 ng/ml) alone, or in combination with 50 µg/ml LPS-depleted Bet v 1.0101 or LPS-depleted nitro-Bet v 1.0101. Each dot represents one donor. The mean and SD for all donors are shown. <i>P</i> values were calculated by one-way ANOVA (*<i>P</i><.05; **<i>P</i><.01).</p

Structural analysis of nitro-Bet v 1.0101.

<p>Crystal structure analysis of monomeric nitro-Bet v 1.0101 revealed that monomeric nitro-Bet v 1.0101 displays the same backbone as Bet v 1.0101 with nitration of Y5, Y66, Y83 and Y150 <i>(A)</i>, the electron density corresponding to the nitration of tyrosine 66 <i>(B)</i>, the detection of two positions of the nitro group on tyrosine 83 <i>(C)</i>, the influence of nitration of Y83 on Asp 69 <i>(D)</i> and the influence of nitration of Y66 on Glu 87 <i>(E)</i>. HPLC-MS analysis of five separately nitrated Bet v 1.0101 samples for calculation of the mean percentage of nitration for each Y showed as main difference between the complete nitrated sample and the monomeric one the additional nitration of Y81 <i>(F)</i>.</p

T-cell line proliferation.

<p>Proliferative responses of Birch pollen (BP)-specific TCLs from BP-allergic donors stimulated with decreasing amounts of LPS-contaminated Bet v 1.0101, mock-Bet v 1.0101 and nitro-Bet v 1.0101 (protein) and stimulated with decreasing amounts of three different LPS-free immunodominant epitopes (peptides) of Bet v 1.0101 containing a tyrosine, in native form or nitrated. The SI was calculated as the ratio between counts per minute (cpm) in stimulated cultures and cpm of the medium control. Each symbol represents a single donor (n = 5), the mean is indicated with a horizontal bar and the SD is represented by the vertical bar. Statistical significance was calculated with One-Way Anova and the Tukey's test per concentration (untreated, mock-nitrated and nitrated protein): <i>P</i> = .2506 for 5 µg/ml protein; <i>P</i> = .1354 for 2.5 µg/ml protein; <i>P = </i>.0815 for 1.25 µg/ml protein and <i>P</i> = .0618 for 0.625 µg/ml protein. Statistical significance was calculated with the <i>t</i>-test for the untreated and nitrated peptide per concentration. No values below <i>P</i> = .0824 could be measured.</p

Systems used for molecular dynamics simulations.

<p>Systems used for molecular dynamics simulations.</p

Cytokine profiles of DCs stimulated with LPS-contaminated allergen.

<p>Cytokine profiles of monocyte-derived DCs <i>(A)</i> and primary DCs <i>(B)</i> stimulated for 24 hours with LPS-contaminated Bet v 1.0101, mock-Bet v 1.0101 or nitro-Bet v 1.0101, nitrated to different degrees. MoDCs were stimulated with 100 µg/ml protein (total LPS contamination of 4 ng/ml) and primDCs with 50 µg/ml protein (total LPS contamination of 2 ng/ml). Each dot represents one donor. The mean and SD for all donors are shown. <i>P</i> values were calculated by one-way ANOVA (*<i>P</i><.05; **<i>P</i><.01; ***<i>P</i><.001).</p

X-ray data collection and model refinement statistics.

<p>X-ray data collection and model refinement statistics.</p

Biochemical characterization.

<p>Oligomerization and aggregation behavior of Bet v 1.0101, mock-Bet v 1.0101 and nitro-Bet v 1.0101 analyzed by size exclusion chromatography (SEC), by gel electrophoresis and by DLS. Highly concentrated sample (15 mg/ml) was loaded onto a Superdex 75 10/300 GL column for SEC analysis. Bet v 1.0101 and mock-Bet v 1.0101 eluted as a monomeric peak, whereas nitro-Bet v 1.0101 showed several peaks corresponding to oligomers. An SDS-PAGE (15%, non-reducing) with the corresponding fractions as eluted during the SEC is shown. Bet v 1.0101 was collected starting from an elution volume of 11 ml, nitro-Bet v 1.0101 was collected starting from an elution volume of 7 ml. <i>(A)</i> Nitro-tyrosine was detected with anti-nitrotyrosine antibody on Western blot and the whole protein was visualized by silver staining on SDS-PAGE. <i>(B)</i> DLS measurements revealed different aggregation states for Bet v 1.0101, mock-Bet v 1.0101 and nitro-Bet v 1.0101. <i>(C)</i>.</p