26 research outputs found
Tracking of Serum Lipid Levels, Blood Pressure, and Body Mass Index from Childhood to Adulthood: The Cardiovascular Risk in Young Finns Study.
Molecular epidemiological analysis of hepatitis A virus strain separated in Fukuoka 2010-the similarity with the strain from a patient infected in Borneo (Kalimantan) island 1999
The assessment of vertebral fractures in elderly women with recent hip fractures: the BREAK Study
Intra-individual lap time variation of the 400-m walk, an early mobility indicator of executive function decline in high-functioning older adults?
Soil application of ash produced by low-temperature fluidized bed gasification: effects on soil nutrient dynamics and crop response
Induction of initial steps of angiogenic differentiation and maturation of endothelial cells by pericytes in vitro and the role of collagen IV
Activation of endothelial cells and recruitment of mural cells define critical steps during the formation of stable vascular elements. Both events are reflected by cocultures of endothelial cells and isolated murine pericyte-like cells and define a versatile platform for the analysis of distinct steps during the angiogenic process in vitro. Isolated pericyte-like cells promote the survival of endothelial cells, induce the assembly of endothelial cells as well as establish direct contacts with forming endothelial alignments. More importantly, they also induce characteristic steps of maturation including the assembly of stable cell-cell junctions, deposition of basement membrane-like matrices and local formation of a central lumen. The presence of pericyte-like cells induces the secretion of extracellular matrices enriched in collagen IV by endothelial cells, which improves endothelial tube formation and provides the adhesive substrate for mural cell recruitment. Collagen-binding integrins contribute differentially to the process, with α1ÎČ1 involved in the adhesion of pericyte-like cells to collagen IV and α2ÎČ1 mainly involved in endothelial cord formation. These data indicate that pericyte-like cells are essential for the survival of endothelial cells, the efficient formation of endothelial alignments as well as initial steps of maturation of capillary-like structures