Fragment of an endogenous inhibitor produced in Escherichia coli for calcium-activated neutral protease (CANP) retains an inhibitory activity

AbstractA C-terminal fragment of an endogenous rabbit liver inhibitor for calcium-activated neutral protease (CANP) was produced in Escherichia coli and its inhibitory activity was examined after purification. The truncated inhibitor (373 amino acid residues), which contains two internal repeat structures, inhibits 2 mol CANP whereas the native liver inhibitor (639 residues), containing four internal repeat structures, inhibits 4 mol CANP. This supports the hypothesis that the repeating unit is the functional unit of inhibition. The results also indicate that post-translational modification of the inhibitor is not essential for inhibition

Heavy quark action on the anisotropic lattice

We investigate the $O(a)$ improved quark action on anisotropic lattice as a potential framework for the heavy quark, which may enable precision computation of hadronic matrix elements of heavy-light mesons. The relativity relations of heavy-light mesons as well as of heavy quarkonium are examined on a quenched lattice with spatial lattice cutoff $a_\sigma^{-1} \simeq$ 1.6 GeV and the anisotropy $\xi=4$. We find that the bare anisotropy parameter tuned for the massless quark describes both the heavy-heavy and heavy-light mesons within 2% accuracy for the quark mass $a_\sigma m_Q < 0.8$, which covers the charm quark mass. This bare anisotropy parameter also successfully describes the heavy-light mesons in the quark mass region $a_\sigma m_Q \leq 1.2$ within the same accuracy. Beyond this region, the discretization effects seem to grow gradually. The anisotropic lattice is expected to extend by a factor $\xi$ the quark mass region in which the parameters in the action tuned for the massless limit are applicable for heavy-light systems with well controlled systematic errors.Comment: 11 pages, REVTeX4, 11 eps figure

The attraction of Tremex apicalis (Hymenoptera, Siricidae, Tremecinae) and its parasitoid Ibalia japonica (Hymenoptera, Ibaliidae) to the fungus Cerrena unicolor

Woodwasps (Hymenoptera: Siricidae) are saproxylic insects and a common forest pest. Siricid woodwasps are classified into two subfamilies: Siricinae and Tremecinae. All known symbiotic fungi of Siricinae are in the genus Amylostereum Boidin while some species of Tremecinae have been observed to have a relationship with the fungus Cerrena unicolor (Bull.) Murrill. Previous studies about the host searching behavior of woodwasps and their parasitoids have focused primarily on the subfamily Siricinae. We analyzed the role of C. unicolor volatiles on the host searching behavior of Tremex apicalis Matsumura (Hymenoptera: Siricidae: Tremecinae) and its parasitoid Ibalia (Tremibalia) japonica Matsumura (Hymenoptera: Ibaliidae). The results of an olfactory response experiment indicated that the females of T. apicalis and its parasitoid find their respective hosts using volatiles from C. unicolor. Using DNA barcode, we identified basidiocarps on the trees infested with T. apicalis. The basidiocarps were all white-rot fungi that cause sapwood decay, including C. unicolor. Two additional species that we identified belonged to genera closely related to C. unicolor. Woodwasp species are known to carry symbiotic fungi in a pair of specialized sacs called mycangia. Notably we found that mycangia-like structures were absent in the abdomens of T. apicalis females. To the best of our knowledge, Xeris spectrum (Linnaeus) (Hymenoptera: Siricidae) is the only reported example of woodwasp species that do not contain symbiotic fungi in their bodies. Our results suggested that: (1) T. apicalis females search for host wood that is already infected with sapwood decaying fungus using volatile compounds; (2) T. apicalis’ female parasitoid also uses volatile compounds from fungus to locate wood that is infested with its potential host

Increased Systemic Glucose Tolerance with Increased Muscle Glucose Uptake in Transgenic Mice Overexpressing RXRγ in Skeletal Muscle

BACKGROUND: Retinoid X receptor (RXR) γ is a nuclear receptor-type transcription factor expressed mostly in skeletal muscle, and regulated by nutritional conditions. Previously, we established transgenic mice overexpressing RXRγ in skeletal muscle (RXRγ mice), which showed lower blood glucose than the control mice. Here we investigated their glucose metabolism. METHODOLOGY/PRINCIPAL FINDINGS: RXRγ mice were subjected to glucose and insulin tolerance tests, and glucose transporter expression levels, hyperinsulinemic-euglycemic clamp and glucose uptake were analyzed. Microarray and bioinformatics analyses were done. The glucose tolerance test revealed higher glucose disposal in RXRγ mice than in control mice, but insulin tolerance test revealed no difference in the insulin-induced hypoglycemic response. In the hyperinsulinemic-euglycemic clamp study, the basal glucose disposal rate was higher in RXRγ mice than in control mice, indicating an insulin-independent increase in glucose uptake. There was no difference in the rate of glucose infusion needed to maintain euglycemia (glucose infusion rate) between the RXRγ and control mice, which is consistent with the result of the insulin tolerance test. Skeletal muscle from RXRγ mice showed increased Glut1 expression, with increased glucose uptake, in an insulin-independent manner. Moreover, we performed in vivo luciferase reporter analysis using Glut1 promoter (Glut1-Luc). Combination of RXRγ and PPARδ resulted in an increase in Glut1-Luc activity in skeletal muscle in vivo. Microarray data showed that RXRγ overexpression increased a diverse set of genes, including glucose metabolism genes, whose promoter contained putative PPAR-binding motifs. CONCLUSIONS/SIGNIFICANCE: Systemic glucose metabolism was increased in transgenic mice overexpressing RXRγ. The enhanced glucose tolerance in RXRγ mice may be mediated at least in part by increased Glut1 in skeletal muscle. These results show the importance of skeletal muscle gene regulation in systemic glucose metabolism. Increasing RXRγ expression may be a novel therapeutic strategy against type 2 diabetes

Induction of apoptotic lesions in liver and lymphoid tissues and modulation of cytokine mRNA expression by acute exposure to deoxynivalenol in piglets

Six 1-month-old piglets were intravenously injected with deoxynivalenol (DON) at the concentration of 1 mg/kg body weight, with three pigs each necropsied at 6 and 24 h post-injection (PI) for investigation of hepatotoxicity and immunotoxicity with special attention to apoptotic changes and cytokine mRNA expression. Histopathological examination of the DON-injected pigs revealed systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and immunohistochemical staining against single-stranded DNA and cleaved caspase-3. The number of TUNEL-positive cells in the thymus and Peyer's patches of the ileum was increased at 24 h PI compared to 6 h PI, but the peak was at 6 h PI in the liver. The mRNA expression of interleukin (IL)-1β, IL-6, IL-18, and tumor necrosis factor (TNF)-α in the spleen, thymus and mesenteric lymph nodes were determined by semi-quantitative RT-PCR, and elevated expression of IL-1β mRNA at 6 h PI and a decrease of IL-18 mRNA at 24 h PI were observed in the spleen. IL-1β and IL-6 mRNA expressions increased significantly at 6 h PI in the thymus, but TNF-α decreased at 6 h PI in the mesenteric lymph nodes. These results show the apoptosis of hepatocytes suggesting the hepatotoxic potential of DON, in addition to an immunotoxic effect on the modulation of proinflammatory cytokine genes in lymphoid organs with extensive apoptosis of lymphocytes induced by acute exposure to DON in pigs

Delaying repatriation: Japanese technicians in early postwar China

Research on the Japanese living in Manchukuo in August 1945 has generally fostered the assumption that all Japanese there wanted to return to Japan as soon as possible. Yet, some made the conscious and voluntary decision to stay, at least for the short to medium term. Among those who chose to delay repatriation were a number of technicians employed by Mantetsu’s (South Manchurian Railroad Company) Ch¯uo Shikenjo. This paper looks at the political and personal realities faced by these technicians when making their decisions as whether to stay or leave in terms of the concepts of voluntary and involuntary repatriation. It shows that the circumstances faced, and consequently the decisions made by the technicians, differed over time. It argues that there were three main reasons behind any decision to stay: pragmatism, a sense of responsibility for Japan’s activities during the war and a sense of loyalty

Pneumonia Caused by Severe Acute Respiratory Syndrome Coronavirus 2 and Influenza Virus: A Multicenter Comparative Study

Background: Detailed differences in clinical information between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia (CP), which is the main phenotype of SARS-CoV-2 disease, and influenza pneumonia (IP) are still unclear. Methods: A prospective, multicenter cohort study was conducted by including patients with CP who were hospitalized between January and June 2020 and a retrospective cohort of patients with IP hospitalized from 2009 to 2020. We compared the clinical presentations and studied the prognostic factors of CP and IP. Results: Compared with the IP group (n = 66), in the multivariate analysis, the CP group (n = 362) had a lower percentage of patients with underlying asthma or chronic obstructive pulmonary disease (P < .01), lower neutrophil-to-lymphocyte ratio (P < .01), lower systolic blood pressure (P < .01), higher diastolic blood pressure (P < .01), lower aspartate aminotransferase level (P < .05), higher serum sodium level (P < .05), and more frequent multilobar infiltrates (P < .05). The diagnostic scoring system based on these findings showed excellent differentiation between CP and IP (area under the receiver operating characteristic curve, 0.889). Moreover, the prognostic predictors were different between CP and IP. Conclusions: Comprehensive differences between CP and IP were revealed, highlighting the need for early differentiation between these 2 pneumonias in clinical settings