Dermatophylosis in Santa Inês sheep from Distrito Federal

Relataram-se quatro casos de dermatofilose em ovinos da raça Santa Inês, no período de um ano. Microscopicamente observaram-se filamentos na forma de "trilho de bonde" e zoósporos nos quatro casos. A tentativa do isolamento do microrganismo foi realizada por meio do método de Haalstra e em apenas um caso obteve-se sucesso, observando-se colônias de aparência lisa, formato circular, cor amarelada e hemolítica em ágar sangue. O exame direto com coloração de Gram mostrou-se um método bastante eficiente na confirmação da presença do microrganismo afetando a epiderme em razão da morfologia típica do agente.Four cases of dermatophylosis were reported in Santa Inês sheep in a study period of one year. Microscopically, septate filaments and coccoid forms zoospores were observed. Attempts to isolate the microorganisms were accomplished using Haastra's method and it was successful in only one case. Dermatophilus congolensis samples have grown on blood agar, colonies where hemolytic, small, round and pigmentation vary from yellow to orange. The gram staining method was efficient to confirm the presence of the microorganism affecting the epidermis due to typical morphology of the agent

Bacterial toxins stimulate Rho-dependent tyrosine phosphorylation of focal adhesion proteins

SIGLEAvailable from British Library Document Supply Centre-DSC:DXN020936 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

1-42 B-amyloid peptide (AB1-42) requires PDK1/nPKCs/Rac 1 pathway to induce neuronal death

1–42 β-Amyloid (Aβ1–42) peptide is a key molecule involved in the development of Alzheimer's disease. Some of its effects are manifested at the neuronal morphological level. These morphological changes involve loss of neurites due to cytoskeleton alterations. However, the mechanism of Aβ1–42 peptide activation of the neurodegenerative program is still poorly understood. Here, Aβ1–42 peptide-induced transduction of cellular death signals through the phosphatidylinositol 3-kinase (PI3K)/phosphoinositol-dependent kinase (PDK)/novel protein kinase C (nPKC)/Rac 1 axis is described. Furthermore, pharmacological inhibition of PDK1 and nPKC activities blocks Rac 1 activation and neuronal cell death. Our results provide insights into an unsuspected connection between PDK1, nPKCs and Rac 1 in the same signal-transduction pathway and points out nPKCs and Rac 1 as potential therapeutic targets to block the toxic effects of Aβ1–42 peptide in neurons.Fil: Manterola, L.. Biodonostia Institute, Hospital Donostia; EspañaFil: Hernando Rodriguez, M.. National Cancer Research Center; EspañaFil: Ruiz, A.. Universidad del Pais Vasco; España. Achucarro Basque Center for Neuroscience; EspañaFil: Apraiz, A.. Universidad del Pais Vasco; EspañaFil: Arrizabalaga, O.. Universidad del Pais Vasco; EspañaFil: Vellon, L.. Fundación Instituto de Investigación Biomédica y Desarrollo Tecnológico (INBIOMED); EspañaFil: Alberdi, E.. Universidad del Pais Vasco; EspañaFil: Cavaliere, F.. Universidad del Pais Vasco; EspañaFil: Lacerda, H.M.. Università degli Studi di Torino; ItaliaFil: Jimenez, S.. Universidad de Sevilla; España. Consejo Superior de Investigaciones Cientificas; EspañaFil: Parada, Luis Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Matute, C.. Universidad del Pais Vasco; EspañaFil: Zugaza, Jose Luis. Universidad del Pais Vasco; España. Bizkaia Science and Technology Park; Españ