Additional file 1: of Enterovirus serotypes in patients with central nervous system and respiratory infections in Viet Nam 1997–2010

Figure S1. The geographic distribution of CNS and respiratory cases included in the current study (TIFF 892 kb

Additional file 3: of Enterovirus serotypes in patients with central nervous system and respiratory infections in Viet Nam 1997–2010

Figure S3. Phylogenetic tree analysis of VP4/VP2 enteroviral sequences. A middle-point rooted tree of VP4/VP2 (420 bp) sequences showing the genetic relationship among species and serotypes of the current study (filled triangles and circles) and with reference prototypes. Filled triangles indicate sequences derived from patients with CNS infection and filled circles indicate sequences derived from patients with respiratory infection. (TIFF 1075 kb

Additional file 2: of Enterovirus serotypes in patients with central nervous system and respiratory infections in Viet Nam 1997–2010

Figure S2. Phylogenetic tree analysis of partial VP1 enteroviral sequences. A middle-point rooted tree of partial VP1 sequences (330 bp) showing the genetic relationship among species and serotypes of the current study (filled triangles) and with reference prototypes. Filled triangles indicate sequences derived from patients with CNS infection. (TIFF 5689 kb

Additional file 1: Table S1. of Development and evaluation of a non-ribosomal random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens

List of 96 FR26RV-Endoh and FR20RV primer sequences. Table S2. Result summary of consensus sequence variations recorded between 2 replicates of 3 tested swabs. Note: NA: not applicable. Figure S1. Screen snapshots showing the mapping results of EV-A71 MiSeq reads to an EV-A71 reference genome of sample ID15; non-ribosomal rPCR assay (bottom panel), non-ribosomal hexanucleotide primers assay (middle panel) and hexanucleotide assay (top panel); the genome sequencing depth is indicated by the Y axis and covered by red circles. Figure S2. Maximum likelihood phylogenetic tree based on completed VP1 nucleotide sequences (891 nt) of EV-A71 strains obtained from this study (in bold red) and representatives retrieved from GenBank. Scale bars indicated numbers of nucleotide substitution per site. CHN, China; USA, United states; TW, Taiwan; NL, Netherlands; MY, Malaysia; KOR, Korean; VN, Vietnam. Figure S3. Maximum likelihood phylogenetic tree based on completed VP1 nucleotide sequences (891 nt) of CV-A16 strains obtained from this study (in bold red) and representatives retrieved from GenBank. Scale bars indicated numbers of nucleotide substitution per site. CHN, China; US, United states; TL, Thailand; JPN, Japan; AUS, Australia; MY, Malaysia; KOR, Korean; VN, Vietnam. (PDF 783 kb

Additional file 1: of Continuous versus intermittent endotracheal cuff pressure control for the prevention of ventilator-associated respiratory infections in Vietnam: study protocol for a randomised controlled trial

Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 checklist: recommended items to address in a clinical trial protocol and related documents. (DOCX 51 kb