Must we live with inclusion bodies?

High expression levels of recombinant proteins do not necessarily mean that the protein must be in inclusion bodies (IBs). This talk will describe some of the more successful solutions to the problem of expressing proteins in an active form in E. col

Brainwashing

"December 1960.""Communist bloc program. China Project"--handwritten on t.p"L2-1154"--handwritten on coverIncludes bibliographical references ( leaves 34-35

Action bias among elite soccer goalkeepers: The case of penalty kicks

In soccer penalty kicks, goalkeepers choose their action before they can clearly observe the kick direction. An analysis of 286 penalty kicks in top leagues and championships worldwide shows that given the probability distribution of kick direction, the optimal strategy for goalkeepers is to stay in the goal's center. Goalkeepers, however, almost always jump right or left. We propose the following explanation for this behavior: because the norm is to jump, norm theory (Kahneman and Miller, 1986) implies that a goal scored yields worse feelings for the goalkeeper following inaction (staying in the center) than following action (jumping), leading to a bias for action. The omission bias, a bias in favor of inaction, is reversed here because the norm here is reversed - to act rather than to choose inaction. The claim that jumping is the norm is supported by a second study, a survey conducted with 32 top professional goalkeepers. The seemingly biased decision making is particularly striking since the goalkeepers have huge incentives to make correct decisions, and it is a decision they encounter frequently. Finally, we discuss several implications of the action/omission bias for economics and management.Decision Making; Uncertainty; Choice Behavior; Sport Psychology; Behavioral Economics; Action Bias; Omission Bias; Commission Bias; Action Effect; Inaction Effect; Actor Effect; Economic Psychology; Heuristics and Biases; Soccer; Goalkeepers; Penalty Kicks; Risk; Norms

Rehabilitating the Client with Monaural Hearing Loss

Rehabilitators receive little, if any, advice about how to manage the client with a monaural hearing loss (MHL). How seriously should they view that condition? The rehabilitation literature devotes very little space to MHL. Indeed, rehabilitation textbooks have almost nothing to say about the effects of MHL can have and about what steps rehabilitators should take in responding to clients who present themselves with MHL. A review of the audiological and ontological research, however, leads to the conclusion that clients with MHL deserve rehabilitators’ careful attentions. These clients should be treated thoughtfully and sympathetically, because MHL’s effects can be, and often are, psychologically, socially, and economically debilitating

SDAP: database and computational tools for allergenic proteins

SDAP (Structural Database of Allergenic Proteins) is a web server that provides rapid, cross-referenced access to the sequences, structures and IgE epitopes of allergenic proteins. The SDAP core is a series of CGI scripts that process the user queries, interrogate the database, perform various computations related to protein allergenic determinants and prepare the output HTML pages. The database component of SDAP contains information about the allergen name, source, sequence, structure, IgE epitopes and literature references and easy links to th

Stereophysicochemical variability plots highlight conserved antigenic areas in Flaviviruses

BACKGROUND: Flaviviruses, which include Dengue (DV) and West Nile (WN), mutate in response to immune system pressure. Identifying escape mutants, variant progeny that replicate in the presence of neutralizing antibodies, is a common way to identify functionally important residues of viral proteins. However, the mutations typically occur at variable positions on the viral surface that are not essential for viral replication. Methods are needed to determine the true targets of the neutralizing antibodies. RESULTS: Stereophysicochemical variability plots (SVPs), 3-D images of protein structures colored according to variability, as determined by our PCPMer program, were used to visualize residues conserved in their physical chemical properties (PCPs) near escape mutant positions. The analysis showed 1) that escape mutations in the flavivirus envelope protein are variable residues by our criteria and 2) two escape mutants found at the same position in many flaviviruses sit above clusters of conserved residues from different regions of the linear sequence. Conservation patterns in T-cell epitopes in the NS3- protease suggest a similar mechanism of immune system evasion. CONCLUSION: The SVPs add another dimension to structurally defining the binding sites of neutralizing antibodies. They provide a useful aid for determining antigenically important regions and designing vaccines

The role of organizational climate in socially embedding construction firms’sustainability goals

Despite the growing interest in sustainability research, little scholarly attention, both conceptual and empirical, has been given to assessing the individual-level behavioural issues that affect sustainability outcomes. Still less research is undertaken to examine the mechanisms by which construction firms enable their sustainability goals to be socially embedded within their organizations to shape the behaviour and attitudes of employees. In an attempt to fill this gap, this paper draws from the extensive literature on organizational climate perspective to explain how organizational-level characteristics and processes can result in a strong, conducive climate that fosters shared perceptions and guide behaviours that are fundamental to the attainment of sustainability goals in organizations. From the critical review of this literature, this paper offers three research propositions and avenues in which they could be tested. The paper concludes with a broadened discussion of the theoretical and practical implications this framework has on advancing the sustainability discourse within CM discipline beyond the current largely technical, policy and institutional foci