105 research outputs found
Association of albumin or protamine to lipoplexes: enhancement of transfection and resistance to serum
The successful application of gene therapy depends on the availability of carriers to efficiently deliver genetic material into target cells. Such efficacy is strongly related to key parameters including serum resistance and protection of DNA.The complexes were tested in terms of their biological activity, in the absence or presence of serum, by following transfection activity. Interaction with plasma proteins was evaluated by immunoblotting, while cytotoxicity was assessed by the Alamar Blue assay. Extent of DNA protection was determined both by using ethidium bromide intercalation and DNase I digestion assays.Our results show that, depending on the charge ratio and on the lipid composition, albumin and protamine can be used (either individually or co-associated) to generate cationic liposome/DNA complexes fulfilling in vivo requirements, while exhibiting high levels of transfection activity. In the present work a novel cationic lipid was tested. It was demonstrated that 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC):cholesterol (Chol) liposomes constitute a very promising carrier for gene delivery as illustrated by their enhancing effect on transfection, as compared with DOTAP-containing liposomes. Moreover, the biological activity of EPOPC-containing complexes is significantly improved upon association of albumin, even in the presence of 60% serum (namely for the 4/1 lipid/DNA charge ratio). Nevertheless, our studies also show that transfection activity mediated by DOTAP-containing complexes can be significantly enhanced upon pre-condensation of DNA with protamine.Co-association of HSA and protamine to lipoplexes ensures a high degree of DNA protection and results in high levels of transfection activity even in the presence of serum. Copyright © 2004 John Wiley & Sons, Ltd
Clinical relevance of incidental colonic uptake 18F-FDG PET/CT. Correlation with colonoscopy and histopathology
O uso do lúdico no ensino de química para deficientes visuais ou baixa visão: utilização da tabela FD14
Anais do II Seminário Seminário Estadual PIBID do Paraná: tecendo saberes / organizado por Dulcyene Maria Ribeiro e Catarina Costa Fernandes — Foz do Iguaçu: Unioeste; Unila, 2014A tabela Periódica organizada e elaborada com características puramente visuais
como formato, distribuição, cor, símbolos, informações numéricas e propriedades com
seguimentos distintos, acaba por excluir de uma vez o deficiente visual. A tabela FD14 ®
apresenta uma proposta onde o aluno, poderá coletar todas as informações contidas em uma
tabela periódica em formato de jogo, sendo a mesma, o principal instrumento na
aprendizagem da química. O modelo de ferramenta proposto foi utilizado por um aluno do 1o
ano do Ensino Médio de 15 anos, deficiente visual desde seu nascimento e inserido no Ensino
Médio regular no Col. Est. Eurídes Brandão. Para êxito da proposta, “João” conseguiu
identificar sem qualquer tipo de auxílio todas as informações que um aluno visual teria
acesso, num processo realmente inclusiv
The pion's electromagnetic form factor at small momentum transfer in full lattice QCD
We compute the electromagnetic form factor of a "pion" with mass m_pi=330MeV
at low values of Q^2\equiv -q^2, where q is the momentum transfer. The
computations are performed in a lattice simulation using an ensemble of the
RBC/UKQCD collaboration's gauge configurations with Domain Wall Fermions and
the Iwasaki gauge action with an inverse lattice spacing of 1.73(3)GeV. In
order to be able to reach low momentum transfers we use partially twisted
boundary conditions using the techniques we have developed and tested earlier.
For the pion of mass 330MeV we find a charge radius given by
_{330MeV}=0.354(31)fm^2 which, using NLO SU(2) chiral perturbation
theory, extrapolates to a value of =0.418(31)fm^2 for a physical pion,
in agreement with the experimentally determined result. We confirm that there
is a significant reduction in computational cost when using propagators
computed from a single time-slice stochastic source compared to using those
with a point source; for m_pi=330MeV and volume (2.74fm)^3 we find the
reduction is approximately a factor of 12.Comment: 20 pages, 3 figure
Composição bromatológica e perfil fermentativo da silagem de capim-elefante obtida em diferentes tipos de silos experimentais e no silo tipo trincheira
Valuation of the Aroeira (Myracrodruon urundeuva Allemão): perspectives on conservation
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