Origin of slow stress relaxation in the cytoskeleton

Dynamically crosslinked semiflexible biopolymers such as the actin cytoskeleton govern the mechanical behavior of living cells. Semiflexible biopolymers nonlinearly stiffen in response to mechanical loads, whereas the crosslinker dynamics allow for stress relaxation over time. Here we show, through rheology and theoretical modeling, that the combined nonlinearity in time and stress leads to an unexpectedly slow stress relaxation, similar to the dynamics of disordered systems close to the glass transition. Our work suggests that transient crosslinking combined with internal stress can explain prior reports of soft glassy rheology of cells, in which the shear modulus increases weakly with frequency.Comment: 6 pages, 4 figure

Targeting Agricultural Drainage to Reduce Nitrogen Losses in a Minnesota Watershed

Agricultural nitrogen losses are the major contributor to nitrogen loads in the Mississippi River, and consequently, to the existence of a hypoxic, or dead, zone in the Gulf of Mexico. Focusing on two small agricultural watersheds in southeast Minnesota, simulation results from the Agricultural Drainage And Pesticide Management (ADAPT) model were combined with a linear-optimization model to evaluate the environmental and economic impact of alternative land-use policies for reducing nitrogen losses. Of particular importance was the studys explicit focus on agricultural subsurface (tile) drainage, which has been identified as the major pathway for agricultural nitrogen losses in the upper Midwest, and the use of drainage-focused abatement policies. Results indicate that tile-drained land plays a key role in nitrogen abatement, and that a combined policy of nutrient management on tile-drained land and retirement of non-drained land is a cost-effective means of achieving a 20- or 30-percent nitrogen-abatement goal. Results also indicate that although it is cost-effective to abate on tile-drained land, it is not cost-effective to undertake policies that plug or remove tile drains from the landscape, regardless of whether the land would be retired or kept in production. Therefore, results imply that although tile-drained land is a major source of nitrogen lost to waterways, it is not cost-effective to remove the land from production or to remove the drainage from the land. Because of its value to agricultural production, it is better to keep tile-drained land in production under nutrient management and focus retirement policies on relatively less-productive, non-drained acres.Environmental Economics and Policy, Land Economics/Use,

Implementation of Simple Measures for Savings Water and Energy Consumption in Kuwait Government Buildings

This paper gives in details the efforts made by the Public Services Department (PSD) to reduce water and energy consumptions in the Ministry of Social Affairs and Labour's (MOSAL) buildings in Kuwait. PSD manages around 125 buildings distributed over 6 governorates. PSD's efforts included the installation of programmable thermostats for A/C units, urging MOSAL's staff to switch off lighting after working hours, replacement of old A/C and lighting systems by newer systems and installation of shutters and solar films for windows, insulation materials for walls and roofs and low-flow water tools for faucets. These efforts reduced the overall water and energy consumptions by 15 and 25%, respectively, in all MOSAL's buildings. Additionally, MOSAL is planning to collaborate with Kuwait Institute for Scientific Research (KISR) to further reduce water and energy consumptions in MOSAL's buildings by optimizing operation strategies and utilizing new water and energy technologies

Attractants for Synanthropic Flies. 2. Response Patterns of House Flies to Attractive Baits on Poultry Ranches

A proteinaceous attractant, prepared by freeze-drying fermented whole egg solids, was found to attract Musca domestica L. and other synanthropic Diptera. In field experiments, a mixture of 2 g of the attractant and 2 g of dichlorvos sugar-bait increased fly collection two-fold on manure substrate in trays set on the ground and three-fold in suspended bait units over the sugar-toxicant bait alone. This increase was due primarily to the increased response of nulliparous and parous females in which vitellogenesis was about to occur. Attractancy of the bait declined sharply after 48 hours of field exposure. The location of bait stations significantly affected the number, sex ratio, and female age structure of the flies collected. Stations collecting the greatest number of flies were generally situated at the ends of the cage rows in sunlight-shade border areas. Fly collections from areas of greatest fly activity were characterized by a high proportion of hoth nulliparous and parous protein-searching females, and the sex ratio in these high-activity zones approached 1. East-west and north-south preferences of certain female age groups were manifested in the collection

Bait Units for Collection of House Flies

Lurtox™4 is a proteinaceous attractant for Musca domestica L. and other synanthropic Diptera (Mulla et al. 1973: Willson and Mulla 1973a) and is easily mixed with commercial poison sugar baits. The mixture of Lurtox and dichlorvos sugar bait (50:50 by wt) can be administered in measurable quantities into compact bait units where dead flies can be easily recovered for counting and for other studies. While developing Lurtox for control of Hippelates eye gnats, Mulla et al. (1973) found that moisture was essential for the emanation of the volatile attractants contained in the bait. Therefore, if the prevalent humidity level is not sufficient to activate the attractant, the bait unit designed for use with the bait mixture should include a moisture sourc

Solution of a Mathematical Model Describing the Change of Hormone Level in Thyroid Using the Laplace Transform

In the present paper, a mathematical model describing the thyroid-pituitary homeostatic mechanism is analyzed for its physiological and clinical significance. The influence of different parameters on the stability behavior of the system is discussed. We have assumed in the present paper that the rate of thyrotropin production is reduced by an amount which is proportional to the blood concentration of thyroxine and also the rate of loss of thyrotropin is proportional to the existing thyrotropin concentration. The stability behavior of the system is analyzed and the possibility of occurrence of periodic solutions is looked into. As the pituitary gland can produce no output in the presence of thyroxine concentration greater than a certain value, we have also included a degenerate form of the equation for thyrotropin production in the present paper. The solutions of the system of governing equations are obtained by applying the Laplace transform. Also, the nature of the solution is interpreted graphically using the Maple12 technique for both stable and unstable behavior

Determinants of drug absorption in different ECMO circuits

Purpose: The aim of this in vitro study was to evaluate potential determinants of drug loss in different ECMO circuits. Methods: Midazolam, morphine, fentanyl, paracetamol, cefazolin, meropenem and vancomycin were injected into three neonatal roller pump, two paediatric roller pump and two clinically used neonatal roller pump circuits, all with a silicone membrane, and two neonatal centrifugal pump circuits with polypropylene hollow-fibre membranes. Serial blood samples were taken from a post-oxygenator site. Drug recovery was calculated as the ratio between the determined and the theoretical maximum concentration. The latter was obtained by dividing dose by theoretical circuit volume. Results: Average drug recoveries at 180 min in three neonatal silicone membrane roller pump circuits were midazolam 0.62%, morphine 23.9%, fentanyl 0.35%, paracetamol 34.0%, cefazolin 84.3%, meropenem 82.9% and vancomycin 67.8%. There was a significant correlation between the lipophilicity of the drug expressed as log P and the extent of drug absorption, p < 0.001. The recovery of midazolam and fentanyl in centrifugal pump circuits with hollow-fibre membrane oxygenator was significantly higher compared to neonatal roller pump circuits with silicone membranes: midazolam 63.4 versus 0.62%, fentanyl 33.8 versus 0.35%, p < 0.001. Oxygenator size and used circuits do not significantly affect drug losses. Conclusions: Significant absorption of drugs occurs in the ECMO circuit, correlating with increased lipophilicity of the drug. Centrifugal pump circuits with hollow-fibre membrane oxygenators show less absorption for all drugs, most pronounced for lipophilic drugs. These results suggest that pharmacokinetics and hence optimal doses of these drugs may be altered during ECMO