On the modelling of biological effects on morphology in estuaries and seas

Morphological modelling aims to explain and predict the changes in rivers, seas and estuaries due to these interaction. In recent history, a lot of progress has been made, especially with stability analysis approaches. However, so far only the physical interactions have been taken into account. It is known however, that biological factors are important to the dynamics of the water systems. In this paper a first step is made in the inclusion of biology into the morphodynamic models. This inclusion is based on the effect that benthic organisms have on the erodibility of the bed. This can easily be included by a change of the critical bed shear stress. These changes in the critical bed shear stress then influence the morphology. This idea has been applied to two cases. The results of the first case indicate that this approach can reproduce the influence of benthic organisms on the mud content of the bed in estuaries. The second case shows that even low numbers of organisms can influence the characteristics of large bed forms

Quantification noise in single cell experiments

In quantitative single-cell studies, the critical part is the low amount of nucleic acids present and the resulting experimental variations. In addition biological data obtained from heterogeneous tissue are not reflecting the expression behaviour of every single-cell. These variations can be derived from natural biological variance or can be introduced externally. Both have negative effects on the quantification result. The aim of this study is to make quantitative single-cell studies more transparent and reliable in order to fulfil the MIQE guidelines at the single-cell level. The technical variability introduced by RT, pre-amplification, evaporation, biological material and qPCR itself was evaluated by using RNA or DNA standards. Secondly, the biological expression variances of GAPDH, TNFα, IL-1β, TLR4 were measured by mRNA profiling experiment in single lymphocytes. The used quantification setup was sensitive enough to detect single standard copies and transcripts out of one solitary cell. Most variability was introduced by RT, followed by evaporation, and pre-amplification. The qPCR analysis and the biological matrix introduced only minor variability. Both conducted studies impressively demonstrate the heterogeneity of expression patterns in individual cells and showed clearly today's limitation in quantitative single-cell expression analysis

Ecologisch gericht suppleren, nu en in de toekomst: het ontwerp meerjarenplan voor monitoring en (toepassingsgericht) onderzoek

Zandsuppleties. Het programma “Ecologisch gericht suppleren, nu en in de toekomst” is een onderzoeks- en monitoringsprogramma voor de middellange termijn (2009-2014) met als doelstelling meer inzicht te krijgen of, en in welke mate, zandsuppleties van invloed zijn op natuurwaarden en op welke wijze zandsuppleties in de nabije toekomst kunnen bijdragen aan de opgaven van veiligheid samen met natuurbehoud en -ontwikkeling. Het programma moet leiden tot een optimalisatie van natuurbescherming van het kustecosysteem en een dynamisch beheer en behoud van de kustlijn

Evaluation of test characteristics of 2 ELISA tests applied to bulk tank milk and claw-trimming records for herd-level diagnosis of bovine digital dermatitis using latent class analysis

Bovine digital dermatitis (DD) is an infectious claw disease with a negative effect on animal welfare and production. Treponema spp. is the main causative agent, and infected animals produce specific antibodies. Our aim was to estimate sensitivity (Se) and specificity (Sp) of 2 ELISA research tests, Medicago's ELISA test and GD Animal Health's in-house ELISA test, for detection of DD-associated Treponema antibodies in bulk tank milk. We used bulk tank milk samples from 154 Nor-wegian dairy cattle herds, 96 from an expected high-prevalence region and 58 from a low-prevalence region. Both tests were evaluated separately against herd-level (aggregated) claw-trimming records extracted from the Norwegian Dairy Herd Recording System using latent class models in a Bayesian analysis. Cutoff values were selected using an explorative approach, and both non-informative priors for all parameters and informative beta priors for distribution of Se and Sp of claw trimming were explored. The estimated (median) true herd-level prevalence of digital dermatitis varied between 24 and 30% in the high-prevalence region and between 3 and 6% in the low-prevalence region. For Medicago's ELISA test, an Se (95% posterior credible interval) of 0.57 (0.32; 0.94) could be achieved without compromis -ing Sp, and for GD Animal Health's in-house ELISA test, an Se of 0.60 (0.37; 0.92) was achieved. Our study showed that both ELISA tests can detect antibodies against DD-associated Treponema spp. in bulk tank milk. However, neither of the 2 ELISA tests produced satisfactory sensitivity without compromising specific-ity. Based on these results, inspection at claw trimming in a chute is necessary for surveillance and control of DD at the herd level in Norway, although these ELISA tests of bulk tank milk might be a useful supplement