Integrated cost-benefit analysis of tsetse control and herd productivity to inform control programs for animal African trypanosomiasis

Animal African trypanosomiasis (AAT) and its tsetse vector are responsible for annual losses estimated in billions of US dollars ($). Recent years have seen the implementation of a series of multinational interventions. However, actors of AAT control face complex resource allocation decisions due to the geographical range of AAT, diversity of ecological and livestock systems, and range of control methods available. The study presented here integrates an existing tsetse abundance model with a bio-economic herd model that captures local production characteristics as well as heterogeneities in AAT incidence and breed. These models were used to predict the impact of tsetse elimination on the net value of cattle production in the districts of Mambwe, in Zambia, and Faro et Déo in Cameroon. The net value of cattle production under the current situation was used as a baseline, and compared with alternative publicly funded control programmes. In Zambia, the current baseline is AAT control implemented privately by cattle owners (Scenario Z0). In Cameroon, the baseline (Scenario C0) is a small-scale publicly funded tsetse control programme and privately funded control at farm level. The model was run for 10 years, using a discount rate of 5%

A model checking approach to the parameter estimation of biochemical pathways

Model checking has historically been an important tool to verify models of a wide variety of systems. Typically a model has to exhibit certain properties to be classed ‘acceptable’. In this work we use model checking in a new setting; parameter estimation. We characterise the desired behaviour of a model in a temporal logic property and alter the model to make it conform to the property (determined through model checking). We have implemented a computational system called MC2(GA) which pairs a model checker with a genetic algorithm. To drive parameter estimation, the fitness of set of parameters in a model is the inverse of the distance between its actual behaviour and the desired behaviour. The model checker used is the simulation-based Monte Carlo Model Checker for Probabilistic Linear-time Temporal Logic with numerical constraints, MC2(PLTLc). Numerical constraints as well as the overall probability of the behaviour expressed in temporal logic are used to minimise the behavioural distance. We define the theory underlying our parameter estimation approach in both the stochastic and continuous worlds. We apply our approach to biochemical systems and present an illustrative example where we estimate the kinetic rate constants in a continuous model of a signalling pathway

Acetazolamide-based fungal chitinase inhibitors

Chitin is an essential structural component of the fungal cell wall. Chitinases are thought to be important for fungal cell wall remodelling, and inhibition of these enzymes has been proposed as a potential strategy for development of novel anti-fungals. The fungal pathogen Aspergillus fumigatus possesses two distinct multi-gene chitinase families. Here we explore acetazolamide as a chemical scaffold for the inhibition of an A. fumigatus ‘plant-type’ chitinase. A co-crystal structure of AfChiA1 with acetazolamide was used to guide synthesis and screening of acetazolamide analogues that yielded SAR in agreement with these structural data. Although acetazolamide and its analogues are weak inhibitors of the enzyme, they have a high ligand efficiency and as such are interesting leads for future inhibitor development

Dissociation in a polymerization model of homochirality

A fully self-contained model of homochirality is presented that contains the effects of both polymerization and dissociation. The dissociation fragments are assumed to replenish the substrate from which new monomers can grow and undergo new polymerization. The mean length of isotactic polymers is found to grow slowly with the normalized total number of corresponding building blocks. Alternatively, if one assumes that the dissociation fragments themselves can polymerize further, then this corresponds to a strong source of short polymers, and an unrealistically short average length of only 3. By contrast, without dissociation, isotactic polymers becomes infinitely long.Comment: 16 pages, 6 figures, submitted to Orig. Life Evol. Biosp

Estimating the nuclear level density with the Monte Carlo shell model

A method for making realistic estimates of the density of levels in even-even nuclei is presented making use of the Monte Carlo shell model (MCSM). The procedure follows three basic steps: (1) computation of the thermal energy with the MCSM, (2) evaluation of the partition function by integrating the thermal energy, and (3) evaluating the level density by performing the inverse Laplace transform of the partition function using Maximum Entropy reconstruction techniques. It is found that results obtained with schematic interactions, which do not have a sign problem in the MCSM, compare well with realistic shell-model interactions provided an important isospin dependence is accounted for.Comment: 14 pages, 3 postscript figures. Latex with RevTex. Submitted as a rapid communication to Phys. Rev.

On Self-Dual Gravity I

(One typo corrected and one incorrect statement removed. Extra details on conserved quantities and symmetry algebras added).Comment: 17 pages, Latex, DAMTP-R92/4

Observation of Thermodynamical Properties in the 162^{162}Dy, 166^{166}Er and 172^{172}Yb Nuclei

The density of accessible levels in the ($^3$He,$\alpha \gamma$) reaction has been extracted for the $^{162}$Dy, $^{166}$Er and $^{172}$Yb nuclei. The nuclear temperature is measured as a function of excitation energy in the region of 0 -- 6 MeV. The temperature curves reveal structures indicating new degrees of freedom. The heat capacity of the nuclear system is discussed within the framework of a canonical ensemble.Comment: 12 pages, 4 figures include

Penatalaksanaan Uveitis Pada Kehamilan dan Pasca Persalinan

Purpose: To report the management of uveitis in pregnancy. Methods: A case report. Result: Themanagement of uveitis in pregnancy is still not clear, so far there was no report from Indonesia. Pregnancyitself is a relative immunosupresive condition where hormonal alteration being changed after delivery. Theuveitis can relaps after delivery. Pregnancy restricts the use of medicine, which can be harmful to motherand the baby. We report the second trimester pregnancy with uveitis, treated with low dose systemicsteroid, cycloplegic and topical steroid. The patient condition remain stable after delivery, suggesting thatlow dose systemic steroid and topical cycloplegic and steroid could give good result. Conclusion : Propermanagement of uveitis in pregnancy can give satisfied result, without anyhazard for the mother and theinfant.

Double Extra Sharp Chopper Increase Efficacy of Phacoemulsification for Hard Mature Cataract Surgery

Objective: To assess the efficacy and safety of a modified double extra sharp chopper for removal of hard cataracts. Design: Prospective non-comparative interventional clinical study. Participants: Forty eyes from 25 patients with hard mature cataract grades 3-4. Methods: The pre-modified Koch chopper was sharpened under the slit lamp to become extra sharp at the tip and inside the edge and 2 mm in length. Patients with hard mature cataract grades 3-4 (grade 4 being the hardest) underwent phacoemulsification by single surgery and were analyzed prospectively. Results: The mean effective phaco time was 23.73 ± 5.75 seconds. Minimal power was facilitated by using horizontal chopping using a self-made double extra sharp chopper. No resistance was encountered while moving the chopper, regardless of cataract persistency. Preoperative BCVA were count fingers (47%), hand movement (35%), and light perception (18%). Postoperative BCVA on day 1and day 7 were 0.57 and 0.95, respectively. There was no difference of effective phaco time among nuclear hardness (P = 0.467), which represent the effectiveness of the extra sharp chopper. Conclusions: The double extra sharp chopper facilitated a safe and rapid visual rehabilitation and maximal subject comfort when doing phacoemulsification for hard mature cataracts. KEYWORDS:: mature cataract, sharp chopper, topical anesthesia, horizontal chopping, phacoemulsificatio

Doppler cooling of a Coulomb crystal

We study theoretically Doppler laser-cooling of a cluster of 2-level atoms confined in a linear ion trap. Using several consecutive steps of averaging we derive, from the full quantum mechanical master equation, an equation for the total mechanical energy of the one dimensional crystal, defined on a coarse-grained energy scale whose grid size is smaller than the linewidth of the electronic transition. This equation describes the cooling dynamics for an arbitrary number of ions and in the quantum regime. We discuss the validity of the ergodic assumption (i.e. that the phase space distribution is only a function of energy). From our equation we derive the semiclassical limit (i.e. when the mechanical motion can be treated classically) and the Lamb-Dicke limit (i.e. when the size of the mechanical wave function is much smaller than the laser wavelength). We find a Fokker-Planck equation for the total mechanical energy of the system, whose solution is in agreement with previous analytical calculations which were based on different assumptions and valid only in their specific regimes. Finally, in the classical limit we derive an analytic expression for the average coupling, by light scattering, between motional states at different energies.Comment: 19 pages, 3 figure