A role for the cleaved cytoplasmic domain of E-cadherin in the nucleus

Cell-cell contacts play a vital role in intracellular signaling, although the molecular mechanisms of these signaling pathways are not fully understood. E-cadherin, an important mediator of cell-cell adhesions, has been shown to be cleaved by γ-secretase. This cleavage releases a fragment of E-cadherin, E-cadherin C-terminal fragment 2 (E-cad/CTF2), into the cytosol. Here, we study the fate and function of this fragment. First, we show that coexpression of the cadherin-binding protein, p120 catenin (p120), enhances the nuclear translocation of E-cad/CTF2. By knocking down p120 with short interfering RNA, we also demonstrate that p120 is necessary for the nuclear localization of E-cad/CTF2. Furthermore, p120 enhances and is required for the specific binding of E-cad/CTF2 to DNA. Finally, we show that E-cad/CTF2 can regulate the p120-Kaiso-mediated signaling pathway in the nucleus. These data indicate a novel role for cleaved E-cadherin in the nucleus

A major cellular substrate for protein kinases, annexin II, is a DNA-binding protein

AbstractWe have screened a human cDNA expression library in λgt11 for clones encoding Alu-binding proteins using direct binding of labeled Alu DNA to recombinant phage lysates fixed on a membrane, and isolated a clone 98% identical in sequence to the well-known substrate of protein kinases, annexin II, which was suggested earlier to play a role in transduction of mitogenic signals and DNA replication. A diagnostic property of annexins is their binding to phospholipids in the presence of calcium ions, and we have found that the interaction of proteins of human nuclear extracts with Alu subsequences is suppressed by Ca/phosphatidylserine liposomes, suggesting overlapping of Ca/phospholipid- and DNA-binding domains in annexin II

Halloysite and Related Mesoporous Carriers for Advanced Catalysis and Drug Delivery

© The Royal Society of Chemistry 2017.For more precise structure control, bottom-up approaches have especially been paid much attention to control nanoscale structures on the basis of the organizing capabilities of the materials themselves. A more advanced concept, nanoarchitectonics, has recently been proposed to promote these nanotechnology flows. Among countless numbers of materials candidates, structurally defined inorganic materials can be nice hosts for development of functional materials structures according to nanoarchitectonics. In this chapter, we mainly focus on introduction of research examples on functionalization of the halloysite nanotubes for catalysis and antibacterial activities. In addition, several examples of hierarchic silica nanostructures for applications such as drug delivery are also briefly introduced

Automatic track recognition for large-angle minimum ionizing particles in nuclear emulsions

We previously developed an automatic track scanning system which enables the detection of large-angle nuclear fragments in the nuclear emulsion films of the OPERA experiment. As a next step, we have investigated this system's track recognition capability for large-angle minimum ionizing particles $(1.0 \leq |tan \theta| \leq 3.5)$. This paper shows that, for such tracks, the system has a detection efficiency of 95$\%$ or higher and reports the achieved angular accuracy of the automatically recognized tracks. This technology is of general purpose and will likely contribute not only to various analyses in the OPERA experiment, but also to future experiments, e.g. on low-energy neutrino and hadron interactions, or to future research on cosmic rays using nuclear emulsions carried by balloons.Comment: 11 pages, 10 figures, accepted by JINS

Random, blocky and alternating ordering in supramolecular polymers of chemically bidisperse monomers

As a first step to understanding the role of molecular or chemical polydispersity in self-assembly, we put forward a coarse-grained model that describes the spontaneous formation of quasi-linear polymers in solutions containing two self-assembling species. Our theoretical framework is based on a two-component self-assembled Ising model in which the bidispersity is parameterized in terms of the strengths of the binding free energies that depend on the monomer species involved in the pairing interaction. Depending upon the relative values of the binding free energies involved, different morphologies of assemblies that include both components are formed, exhibiting paramagnetic-, ferromagnetic- or anti ferromagnetic-like order,i.e., random, blocky or alternating ordering of the two components in the assemblies. Analyzing the model for the case of ferromagnetic ordering, which is of most practical interest, we find that the transition from conditions of minimal assembly to those characterized by strong polymerization can be described by a critical concentration that depends on the concentration ratio of the two species. Interestingly, the distribution of monomers in the assemblies is different from that in the original distribution, i.e., the ratio of the concentrations of the two components put into the system. The monomers with a smaller binding free energy are more abundant in short assemblies and monomers with a larger binding affinity are more abundant in longer assemblies. Under certain conditions the two components congregate into separate supramolecular polymeric species and in that sense phase separate. We find strong deviations from the expected growth law for supramolecular polymers even for modest amounts of a second component, provided it is chemically sufficiently distinct from the main one.Comment: Submitted to Macromolecules, 6 figures. arXiv admin note: substantial text overlap with arXiv:1111.176

Vacuum brazing of titanium alloy to stainless steel enhance by fiber laser surface texturing

A method for improving the brazing joining strength of Titanium alloy/Stainless steel fabricated through fibre laser surface texturing is introduced because it is a simple process that does not require the fabrication of complicated interlayers. However, previous research shows that a milimeter scale was fabricated by surface modification for dissimilar brazing join, yielding insignificant results and limiting the application and degree of enhancement. Fiber laser ablation was used in this study to create microscale periodic patterns (grooves) on a stainless steel surface. No defect or damage induced during laser surface texturing process. The groove dimension was tunable by controlling the laser parameters. Vacuum brazing of Ti6Al4V to 316L stainless steel with surface texturing, the average joint strength was 22.1 MPa, 34% of increase of joining strength compared to unprocessed flat surface. The combination of laser surface texturing and brazing proven effectively on joining strength enhancement