Superantigen as a Promising Immunotherapy Treatment against Cancer: A Review Article

Superantigens are toxins produced in hosts by some pathogenic microbes as a mechanism to sustain their pathogenicity. Superantigens are highly resistant to degradation by proteases enzyme, heat denaturation, and cause many dangerous diseases that in severe cases lead to death. Superantigens are atypical antigens that stimulate a profound proliferation of polyclonal T cells at low concentrations. All superantigens have a standard architecture even though they differ in amino acid sequences and are divided into two groups: endogenous superantigens and exogenous superantigens. The major histocompatibility complex molecules on antigen-presenting cells and T cell receptors on T cells are the major players in identifying foreign antigens. Superantigens can bind nonspecifically to both the major histocompatibility complex class II molecules and T cell receptors and form a trimolecular complex. Superantigens do not follow conventional antigens processing and presentation; they bind as intact macromolecules outside the antigen-binding groove of the major histocompatibility complex class II and to Vβ of T cells receptors. Thus, triggering an excessive release of proinflammatory cytokines, radiotherapy and chemotherapy often develop radio/drug resistance. Hence, cancer immunotherapy has been mainly considered as it instigates the patient’s immune system to fight cancer. Superantigens are one of the most potent T cell mitogens as 0.1 pg/ml is adequate to excite T lymphocytes. Accordingly, extensive in-vivo/in-vitro investigations have been conducted on the potential role of superantigens in eradicating tumors. The safety and effectiveness of superantigens as a cancer treatment have been verified in many clinical trials. Nevertheless, the vast inflammation after the potent T cell activation is known to promote diseases, including cancer. This paper reviews the potentiality of superantigen as an immunotherapy treatment against cancer

Superantigen as a Promising Immunotherapy Treatment against Cancer: A Review Article

Superantigens are toxins produced in hosts by some pathogenic microbes as a mechanism to sustain their pathogenicity. Superantigens are highly resistant to degradation by proteases enzyme, heat denaturation, and cause many dangerous diseases that in severe cases lead to death. Superantigens are atypical antigens that stimulate a profound proliferation of polyclonal T cells at low concentrations. All superantigens have a standard architecture even though they differ in amino acid sequences and are divided into two groups: endogenous superantigens and exogenous superantigens. The major histocompatibility complex molecules on antigen-presenting cells and T cell receptors on T cells are the major players in identifying foreign antigens. Superantigens can bind nonspecifically to both the major histocompatibility complex class II molecules and T cell receptors and form a trimolecular complex. Superantigens do not follow conventional antigens processing and presentation; they bind as intact macromolecules outside the antigen-binding groove of the major histocompatibility complex class II and to Vβ of T cells receptors. Thus, triggering an excessive release of proinflammatory cytokines, radiotherapy and chemotherapy often develop radio/drug resistance. Hence, cancer immunotherapy has been mainly considered as it instigates the patient’s immune system to fight cancer. Superantigens are one of the most potent T cell mitogens as 0.1 pg/ml is adequate to excite T lymphocytes. Accordingly, extensive in-vivo/in-vitro investigations have been conducted on the potential role of superantigens in eradicating tumors. The safety and effectiveness of superantigens as a cancer treatment have been verified in many clinical trials. Nevertheless, the vast inflammation after the potent T cell activation is known to promote diseases, including cancer. This paper reviews the potentiality of superantigen as an immunotherapy treatment against cancer

Identification of key claudin genes associated with survival prognosis and diagnosis in colon cancer through integrated bioinformatic analysis

The claudin multigene family is associated with various aberrant physiological and cellular signaling pathways. However, the association of claudins with survival prognosis, signaling pathways, and diagnostic efficacy in colon cancer remains poorly understood.Methods: Through the effective utilization of various bioinformatics methods, including differential gene expression analysis, gene set enrichment analysis protein-protein interaction (PPI) network analysis, survival analysis, single sample gene set enrichment analysis (ssGSEA), mutational variance analysis, and identifying receiver operating characteristic curve of claudins in The Cancer Genome Atlas colon adenocarcinoma (COAD).Results: We found that: CLDN2, CLDN1, CLDN14, CLDN16, CLDN18, CLDN9, CLDN12, and CLDN6 are elevated in COAD. In contrast, the CLDN8, CLDN23, CLDN5, CLDN11, CLDN7, and CLDN15 are downregulated in COAD. By analyzing the public datasets GSE15781 and GSE50760 from NCBI-GEO (https://www.ncbi.nlm.nih.gov/geo/), we have confirmed that CLDN1, CLDN2, and CLDN14 are significantly upregulated and CLDN8 and CLDN23 are significantly downregulated in normal colon, colon adenocarcinoma tumor, and liver metastasis of colon adenocarcinoma tissues from human samples. Various claudins are mutated and found to be associated with diagnostic efficacy in COAD.Conclusion: The claudin gene family is associated with prognosis, immune regulation, signaling pathway regulations, and diagnosis of COAD. These findings may provide new molecular insight into claudins in the treatment of colon cancer

EFFECT OF CHLORIDE CONCENTRATION IN SOIL ON REINFORCEMENT CORROSION

ABSTRACT This paper presents results of a study conducted to evaluate the effect of chloride concentration in soil on corrosion of reinforcing steel in concrete. Concrete specimens prepared with Type I, Type V and silica fume cements were exposed to soil with a chloride concentration of up to 3%. The effect of chloride concentration in soil on corrosion of reinforcing steel was evaluated by measuring corrosion potentials and corrosion current density. After 18 months of exposure, the concrete specimens were broken and the extent of corrosion of the reinforcing steel was examined and the gravimetric weight loss due to corrosion was assessed. As expected, the degree of corrosion increased with the chloride concentration in the soil. The type of cement also influenced the reinforcement corrosion. For a particular chloride concentration, least reinforcement corrosion was noted in the silica fume cement concrete followed by Type I and Type V cement concrete. Based on the data developed in this study chloride threshold in soil for the three types of cements are suggested

EFFECT OF CHLORIDE CONCENTRATION IN SOIL ON REINFORCEMENT CORROSION

ABSTRACT This paper presents results of a study conducted to evaluate the effect of chloride concentration in soil on corrosion of reinforcing steel in concrete. Concrete specimens prepared with Type I, Type V and silica fume cements were exposed to soil with a chloride concentration of up to 3%. The effect of chloride concentration in soil on corrosion of reinforcing steel was evaluated by measuring corrosion potentials and corrosion current density. After 18 months of exposure, the concrete specimens were broken and the extent of corrosion of the reinforcing steel was examined and the gravimetric weight loss due to corrosion was assessed. As expected, the degree of corrosion increased with the chloride concentration in the soil. The type of cement also influenced the reinforcement corrosion. For a particular chloride concentration, least reinforcement corrosion was noted in the silica fume cement concrete followed by Type I and Type V cement concrete. Based on the data developed in this study chloride threshold in soil for the three types of cements are suggested