On the relevance of polyynyl-substituted PAHs to astrophysics

We report on the absorption spectra of the polycyclic aromatic hydrocarbon (PAH) molecules anthracene, phenanthrene, and pyrene carrying either an ethynyl (-C2H) or a butadiynyl (-C4H) group. Measurements were carried out in the mid infrared at room temperature on grains embedded in CsI pellets and in the near ultraviolet at cryogenic temperature on molecules isolated in Ne matrices. The infrared measurements show that interstellar populations of polyynyl-substituted PAHs would give rise to collective features in the same way non-substituted PAHs give rise to the aromatic infrared bands. The main features characteristic of the substituted molecules correspond to the acetylenic CH stretching mode near 3.05 mum and to the almost isoenergetic acetylenic CCH in- and out-of-plane bending modes near 15.9 mum. Sub-populations defined by the length of the polyynyl side group cause collective features which correspond to the various acetylenic CC stretching modes. The ultraviolet spectra reveal that the addition of an ethynyl group to a non-substituted PAH molecule results in all its electronic transitions being redshifted. Due to fast internal energy conversion, the bands at shorter wavelengths are significantly broadened. Those at longer wavelengths are only barely affected in this respect. As a consequence, their relative peak absorption increases. The substitution with the longer butadiynyl chain causes the same effects with a larger magnitude, resulting in the spectra to show a prominent if not dominating pi-pi* transition at long wavelength. After discussing the relevance of polyynyl-substituted PAHs to astrophysics, we conclude that this class of highly conjugated, unsaturated molecules are valid candidates for the carriers of the diffuse interstellar bands.Comment: 29 pages, 9 figures, accepted for publication in ApJ 2 April 201

Solid-phase synthesis and characterization of n-terminally elongated Aβ-3-x-peptides

In addition to the prototypic amyloid-beta (A beta) peptides A beta(1-40) and A beta(1-42), several A beta variants differing in their amino and carboxy termini have been described. Synthetic availability of an A beta variant is often the key to study its role under physiological or pathological conditions. Herein, we report a protocol for the efficient solid-phase peptide synthesis of the N-terminally elongated Ab-peptides A beta(-3-38), A beta(-3-40), and A beta(-3-42). Biophysical characterization by NMR spectroscopy, CD spectroscopy, an aggregation assay, and electron microscopy revealed that all three peptides were prone to aggregation into amyloid fibrils. Immunoprecipitation, followed by mass spectrometry, indicated that A beta(-3-38) and A beta(-3-40) are generated by transfected cells even in the presence of a tripartite beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) inhibitor. The elongated Ab peptides starting at Val(-3) can be separated from N-terminally-truncated A beta forms by high-resolution isoelectric-focusing techniques, despite virtually identical isoelectric points. The synthetic A beta variants and the methods presented here are providing tools to advance our understanding of the potential roles of N-terminally elongated A beta variants in Alzheimer's disease

The second flight of the SUNRISE balloon-borne solar observatory: overview of instrument updates, the flight, the data and first results

The SUNRISE balloon-borne solar observatory, consisting of a 1~m aperture telescope that provided a stabilized image to a UV filter imager and an imaging vector polarimeter, carried out its second science flight in June 2013. It provided observations of parts of active regions at high spatial resolution, including the first high-resolution images in the Mg~{\sc ii}~k line. The obtained data are of very high quality, with the best UV images reaching the diffraction limit of the telescope at 3000~\AA\ after Multi-Frame Blind Deconvolution reconstruction accounting for phase-diversity information. Here a brief update is given of the instruments and the data reduction techniques, which includes an inversion of the polarimetric data. Mainly those aspects that evolved compared with the first flight are described. A tabular overview of the observations is given. In addition, an example time series of a part of the emerging active region NOAA AR~11768 observed relatively close to disk centre is described and discussed in some detail. The observations cover the pores in the trailing polarity of the active region, as well as the polarity inversion line where flux emergence was ongoing and a small flare-like brightening occurred in the course of the time series. The pores are found to contain magnetic field strengths ranging up to 2500~G and, while large pores are clearly darker and cooler than the quiet Sun in all layers of the photosphere, the temperature and brightness of small pores approach or even exceed those of the quiet Sun in the upper photosphere.Comment: Accepted for publication in The Astrophysical Journa

Magneto-static modeling from SUNRISE/IMaX : application to an active region observed with SUNRISE II

Magneto-static models may overcome some of the issues facing force-free magnetic field extrapolations. So far they have seen limited use and have faced problems when applied to quiet-Sun data. Here we present a first application to an active region. We use solar vector magnetic field measurements gathered by the IMaX polarimeter during the flight of the \sunrise{} balloon-borne solar observatory in June 2013 as boundary condition for a magneto-static model of the higher solar atmosphere above an active region. The IMaX data are embedded in active region vector magnetograms observed with SDO/HMI. This work continues our magneto-static extrapolation approach, which has been applied earlier ({\it Paper I}) to a quiet Sun region observed with \sunrise{} I. In an active region the signal-to-noise-ratio in the measured Stokes parameters is considerably higher than in the quiet Sun and consequently the IMaX measurements of the horizontal photospheric magnetic field allow us to specify the free parameters of the model in a special class of linear magneto-static equilibria. The high spatial resolution of IMaX (110-130 km, pixel size 40 km) enables us to model the non-force-free layer between the photosphere and the mid chromosphere vertically by about 50 grid points. In our approach we can incorporate some aspects of the mixed beta layer of photosphere and chromosphere, e.g., taking a finite Lorentz force into account, which was not possible with lower resolution photospheric measurements in the past. The linear model does not, however, permit to model intrinsic nonlinear structures like strongly localized electric currents.PostprintPeer reviewe

Mechanism and specificity of pentachloropseudilin-mediated inhibition of myosin motor activity.

Here, we report that the natural compound pentachloropseudilin (PClP) acts as a reversible and allosteric inhibitor of myosin ATPase and motor activity. IC(50) values are in the range from 1 to 5 μm for mammalian class-1 myosins and greater than 90 μm for class-2 and class-5 myosins, and no inhibition was observed with class-6 and class-7 myosins. We show that in mammalian cells, PClP selectively inhibits myosin-1c function. To elucidate the structural basis for PClP-induced allosteric coupling and isoform-specific differences in the inhibitory potency of the compound, we used a multifaceted approach combining direct functional, crystallographic, and in silico modeling studies. Our results indicate that allosteric inhibition by PClP is mediated by the combined effects of global changes in protein dynamics and direct communication between the catalytic and allosteric sites via a cascade of small conformational changes along a conserved communication pathway

Power spectrum of turbulent convection in the solar photosphere

The solar photosphere provides us with a laboratory for understanding turbulence in a layer where the fundamental processes of transport vary rapidly and a strongly superadiabatic region lies very closely to a subadiabatic layer. Our tools for probing the turbulence are high-resolution spectropolarimetric observations such as have recently been obtained with the two sunrise missions, and numerical simulations. Our aim is to study photospheric turbulence with the help of Fourier power spectra that we compute from observations and simulations. We also attempt to explain some properties of the photospheric overshooting flow with the help of its governing equations and simulations. We find that quiet-Sun observations and smeared simulations exhibit a power-law behavior in the subgranular range of their Doppler velocity power spectra with an index of$~\approx -2$. The unsmeared simulations exhibit a power-law index of$~\approx -2.25$. The smearing considerably reduces the extent of the power-law-like portion of the spectra. Therefore, the limited spatial resolution in some observations might eventually result in larger uncertainties in the estimation of the power-law indices. The simulated vertical velocity power spectra as a function of height show a rapid change in the power-law index from the solar surface to $300$~km above it. A scale-dependent transport of the vertical momentum occurs. At smaller scales, the vertical momentum is more efficiently transported sideways than at larger scales. This results in less vertical velocity power transported upward at small scales than at larger scales and produces a progressively steeper vertical velocity power law below $180$ km. Above this height, the gravity work progressively gains importance at all scales, making the atmosphere progressively more hydrostatic and resulting in a gradually less steep power law.Comment: 10 pages, 7 figures, Accepted in A and

Small-scale solar magnetic fields

As we resolve ever smaller structures in the solar atmosphere, it has become clear that magnetism is an important component of those small structures. Small-scale magnetism holds the key to many poorly understood facets of solar magnetism on all scales, such as the existence of a local dynamo, chromospheric heating, and flux emergence, to name a few. Here, we review our knowledge of small-scale photospheric fields, with particular emphasis on quiet-sun field, and discuss the implications of several results obtained recently using new instruments, as well as future prospects in this field of research.Comment: 43 pages, 18 figure

DAF-12 Regulates a Connected Network of Genes to Ensure Robust Developmental Decisions

The nuclear receptor DAF-12 has roles in normal development, the decision to pursue dauer development in unfavorable conditions, and the modulation of adult aging. Despite the biologic importance of DAF-12, target genes for this receptor are largely unknown. To identify DAF-12 targets, we performed chromatin immunoprecipitation followed by hybridization to whole-genome tiling arrays. We identified 1,175 genomic regions to be bound in vivo by DAF-12, and these regions are enriched in known DAF-12 binding motifs and act as DAF-12 response elements in transfected cells and in transgenic worms. The DAF-12 target genes near these binding sites include an extensive network of interconnected heterochronic and microRNA genes. We also identify the genes encoding components of the miRISC, which is required for the control of target genes by microRNA, as a target of DAF-12 regulation. During reproductive development, many of these target genes are misregulated in daf-12(0) mutants, but this only infrequently results in developmental phenotypes. In contrast, we and others have found that null daf-12 mutations enhance the phenotypes of many miRISC and heterochronic target genes. We also find that environmental fluctuations significantly strengthen the weak heterochronic phenotypes of null daf-12 alleles. During diapause, DAF-12 represses the expression of many heterochronic and miRISC target genes, and prior work has demonstrated that dauer formation can suppress the heterochronic phenotypes of many of these target genes in post-dauer development. Together these data are consistent with daf-12 acting to ensure developmental robustness by committing the animal to adult or dauer developmental programs despite variable internal or external conditions