NH4+ secretion in inner medullary collecting duct in potassium deprivation: Role of colonic H+-K+-ATPase

NH4+ secretion in inner medullary collecting duct in potassium deprivation: Role of colonic H+-K+-ATPase.BackgroundIn K+ deprivation (KD), gastric (g) H+-K+-ATPase (HKA) is suppressed, whereas colonic (c) HKA is induced in the terminal inner medullary collecting duct (IMCD). We hypothesized that in KD, cHKA is induced and can mediate the secretion of NH4+.MethodsRats were sacrificed after 2, 3, 6, or 14 days on regular (NML) or K+-free (KD) diet. mRNA expression of HKA isoforms in terminal inner medulla was examined and correlated with NH4+ secretion in perfused IMCD in vitro.ResultsUrinary NH4+ excretion increased after K+-free diet for six days. In terminal inner medulla, cHKA expression was strongly induced, whereas gHKA expression was decreased. NH4+ secretion increased by 62% in KD (JtNH4+ 0.57 vs. 0.92 pmol/min/mm tubule length, P < 0.001). Ouabain (1 mM) in perfusate inhibited NH4+ secretion in KD by 45% (P < 0.002) but not in NML. At luminal pH 7.7, which inhibits NH3 diffusion, NH4+ secretion in IMCD was 140% higher in KD (0.36 vs. 0.15, P < 0.03) and was sensitive to ouabain. ROMK-1 mRNA expression was induced in parallel with cHKA in inner medulla.ConclusionsThese data suggest that in KD, cHKA replaces gHKA and mediates enhanced secretion of NH4+ (and H+) into the lumen facilitated by K+ recycling through ROMK-1

Porphyrin-nanoassembled fiber-optic gas sensor fabrication: Optimization of parameters for sensitive ammonia gas detection

Highly sensitive fiber-optic ammonia gas sensors were fabricated via layer-by-layer deposition of poly(diallyldimethylammonium chloride) (PDDA) and tetrakis(4-sulfophenyl)porphine (TSPP) onto the surface of the core of a hard-clad multimode fiber that was stripped of its polymer cladding. The effects of film thickness, length of sensing area, and depth of evanescent wave penetration were investigated to clearly understand the sensor performance. The sensitivity of the fiber-optic sensor to ammonia was linear in the concentration range of 0.5–50 ppm and the response and recovery times were less than 3 min, with a limit of detection of 0.5 ppm, when a ten-cycle PDDA/TSPP film was assembled on the surface of the core along a 1 cm-long stripped section of the fiber. The sensor’s response towards ammonia was also checked under different relative humidity conditions and a simple statistical data treatment approach, principal component analysis, demonstrated the feasibility of ammonia sensing in environmental relative humidity ranging from dry 7% to highly saturated 80%. Penetration depths of the evanescent wave for the optimal sensor configuration were estimated to be 30 and 33 nm at wavelengths of 420 and 706 nm, which are in a good agreement with the thickness of the 10-cycle deposited film (ca. 30 nm)

Comments on gluon 6-point scattering amplitudes in N=4 SYM at strong coupling

We use the AdS/CFT prescription of Alday and Maldacena \cite{am} to analyze gluon 6-point scattering amplitudes at strong coupling in ${\cal N}=4$ SYM. By cutting and gluing we obtain AdS 6-point amplitudes that contain extra boundary conditions and come close to matching the field theory results. We interpret them as parts of the field theory amplitudes, containing only certain diagrams. We also analyze the collinear limits of 6- and 5-point amplitudes and discuss the results.Comment: 35 pages, 7 figures, latex, references adde

In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis

The central channel of the nuclear pore complex (NPC) is occupied by non-structured polypeptides with a high content of Phe-Gly (FG) motifs. This protein-rich environment functions as an entropic barrier that prevents the passage of molecules, as well as the binding sites for karyopherins, to regulate macromolecular traffic between the nucleoplasm and the cytoplasm. In this study, we expressed individual Nups fused with a crowding-sensitive probe (GimRET) to determine the spatial distribution of protein-rich domains within the central channel in vivo, and characterize the properties of the entropic barrier. Analyses of the probe signal revealed that the central channel contains two protein-rich domains at both the nucleoplasmic and cytoplasmic peripheries, and a less-crowded central cavity. Karyopherins and other soluble proteins are not the constituents of the protein-rich domains. The time-lapse observation of the post-mitotic reassembly process also revealed how individual protein-rich domains are constructed by a sequential assembly of nucleoporins

Effect of a baffle divertor structure on neutral hydrogen and helium transport in the Large Helical Device

Control of the peripheral plasma density is one of urgent tasks for improving the plasma performance in the Large Helical Device. Test modules with a baffle divertor structure have been installed in the inboard side of the torus for controlling the neutral density in the plasma periphery. Neutral hydrogen and helium transport in an original open divertor and the baffle divertor configurations have been monitored with filtered CCD cameras to observe the intensity profile of visible emission by neutral hydrogen (Hα) and helium (HeI). A detailed analysis using a three-dimensional neutral particle transport simulation code reasonably explains the dependence of the observed intensity profiles on the divertor and the magnetic configurations. It also predicts that the baffle divertor significantly reduces the emission of neutral hydrogen in the plasma periphery in the inboard side, and the compression of neutral helium density by the baffle divertor is comparable to that of hydrogen

Effect of divertor legs on neutral particle and impurity retention for a closed helical divertor configuration in the Large Helical Device

A closed helical divertor (CHD) has been designed for efficient particle control in the plasma periphery and for retaining neutral particles and impurity ions in the divertor region. The effect of impurity retention by divertor legs for the CHD configuration is investigated from the viewpoints of neutral impurity transport and force balance of impurity ions along magnetic field lines. A fully three-dimensional neutral particle transport simulation proves that the plasma on the divertor legs is effective for retaining neutral particles/impurities in the CHD region. A one-dimensional impurity ion transport analysis predicts that friction force by plasma flow from the main plasma sweep impurity ions toward the divertor plates even in high neutral density case in which a steep temperature gradient is formed. It shows that the CHD configuration is promising for enhancing LHD plasma performance by effective control of the neutral particles and the impurity ions in the plasma periphery

Study of the effect of a closed divertor configuration on neutral particle control in the LHD plasma periphery

An optimized closed divertor configuration for effective particle control in LHD is proposed from the viewpoints of the distribution of the strike points and neutral particle transport. Calculations of the distribution of the strike points indicate that 50% of the strike points locate in the inboard side of the torus in a standard magnetic configuration (Rax = 3.60 m). The ratio increases to 80% by installing target plates near lower/upper ports. A three-dimensional neutral particle transport simulation shows that installation of closed divertor components with the target plates raise the neutral pressure in the inboard side by more than one order of magnitude compared to that in the present open divertor case. The analysis of the neutral particle transport predicts that enhancement of the neutral pressure becomes moderate in outward shift configurations (Rax > 3.75 m)

Chain and mirophase-separated structures of ultrathin polyurethane films

Measurements are presented how chain and microphase-separated structures of ultrathin polyurethane (PU) films are controlled by the thickness. The film thickness is varied by a solution concentration for spin coating. The systems are PUs prepared from commercial raw materials. Fourier-transform infrared spectroscopic measurement revealed that the degree of hydrogen bonding among hard segment chains decreased and increased with decreasing film thickness for strong and weak microphase separation systems, respectively. The microphase-separated structure, which is formed from hard segment domains and a surrounding soft segment matrix, were observed by atomic force microscopy. The size of hard segment domains decreased with decreasing film thickness, and possibility of specific orientation of the hard segment chains was exhibited for both systems. These results are due to decreasing space for the formation of the microphase-separated structure.IUMRS-ICA 2008 Symposium Sessions X and Y. Soft Matter Science, 9-13 December, Nagoya, Japa

Molecular Mobility of Soft Segment of Polyurethane Elastomers under Elongation

Abstract. In this study, we investigated molecular mobility of a soft segment in the poly(oxypropylene) glycol (PPG), 4,4\u27-diphenylmethane diisocyanate (MDI) and 1,4-butane diol (BD)-based polyurethane elastomers (PUE) with and without elongation by dynamic viscoelastic property measurement and pulse nuclear magnetic resonance (NMR) measurement. The peak position of the loss tangent (tand) curves shifted to the lower temperature region with increasing elongation. In the pulse NMR measurement, the long spin-spin relaxation time {T2) component appeared at -18.0 (e = 0) and -26.0 °C (e =1.5), respectively, with increasing temperature. Since this temperature seems to be related to the glass transition temperature (Jg) of the soft segment in the PUE, it is likely to consider that the Tg decreased with increasing strain. These results might be attributed that the size of cooperative motion during the glass transition decrease due to the orientation of the soft segment, and the soft segment phase approach to a pure phase on account of the extraction of the hard segment from the soft segment phase.THE XV INTERNATIONAL CONGRESS ON RHEOLOGY: The Society of Rheology 80th Annual Meeting : Monterey (California), 3?8 August 200

Investigation of the Helical Divertor Function and the Future Plan of a Closed Divertor for Efficient Particle Control in the LHD Plasma Periphery

The function of the divertor plasmas on the particle control in the plasma periphery is investigated from viewpoints of magnetic field line structures and neutral particle transport in the Large Helical Device (LHD). It shows that the particle and heat deposition on the divertor plate arrays are qualitatively explained by the distribution of strike points calculated by magnetic field line tracing including a particle diffusion effect. Control of neutral particle fueling from the divertor plates is a critical issue for sustaining long-pulse discharges and achieving superdense core plasmas. The behavior of neutral particles in the plasma periphery has been investigated by Hα emission measurements and a neutral particle transport simulation. It reveals that gas fueling from the toroidally distributed divertor plates heated by protons accelerated by ion cyclotron resonance frequency wave is necessary for explaining measurements in a long-pulse discharge, and the spatial profile of the neutral particle density in the plasma periphery in various magnetic configurations is explained by the strike point distribution. Based on these analyses, a closed helical divertor configuration optimized for the intrinsic magnetic field line structure in the plasma periphery is proposed for efficient particle control and heat load reduction on the divertor plates