Developing Mathematical Knowledge and Skills through the Awareness Approach of Teaching and Learning

Every object we think of or encounter, whether a natural or human-made, has a regular or irregular shape. In its own intrinsic conceptual design, it has elements of mathematics, science, engineering, and arts, etc., which are part of the object’s geometric shape, form and structure. Geometry is not only an important part of mathematics, but it is also an important part of daily life.  However, geometry is challenging for some students, even high-achieving students.  One way to help students understand geometry and its relevance in life is to engage students to discover them cognitively, then to research and identify them in real world examples and then to relate them to past, present, and future innovations that improved our way of thinking about ourselves and the world around us.  This interdisciplinary activity uses the Developmental Awareness Approach of Teaching and Learning (DAATL) to help students discover principles, acquire knowledge, and learn mathematical concepts including surface area, volume, dimensions, regular and irregular plane figures, solid polygons (regular polygons and polyhedra), thinking design, and graph making, etc.  It is designed to help students become acquainted with the most useful and familiar parts of mathematical geometry and its application in daily life through connections with disciplines such as science, engineering, art, design, and social studies.  The Development Awareness Approach of Teaching and Learning (DAATL) capitalizes on student's natural curiosity, inclination to comprehend as well as students love of drawing, doodling, painting, thinking and talking.  Throughout the learning process, students are engaged in authentic learning activities by real and concrete doing with clear purposes, thinking analytically, and evaluating their understanding of texts and ideas orally, in drawing, and in writing. This approach of teaching and learning has been tried and modified to ensure maximum effectiveness of acquiring understanding of the intended learning concepts.   The activities can be used with students in elementary school up to 2-year college levels. Keywords: Geometry, Learning Math, Developmental Discovery Approach, Active Learning, Student’s Active Engagement

A HER2 selective theranostic agent for surgical resection guidance and photodynamic therapy

In many cancers early intervention involves surgical resection of small localised tumour masses. Inadequate resection leads to recurrence whereas overzealous treatment can lead to organ damage. This work describes production of a HER2 targeting antibody Fab fragment dual conjugated to achieve both real time near-infrared fluorescent imaging and photodynamic therapy. The use of fluorescence emission from a NIR-dye could be used to guide resection of tumour bulk, for example during endoscopic diagnosis for oesophago-gastric adenocarcinoma, this would then be followed by activation of the photodynamic therapeutic agent to destroy untreated localised areas of cancer infiltration and tumour infiltrated lymph nodes. This theranostic agent was prepared from the Fab fragment of trastuzumab initially by functional disulfide re-bridging and site-specific click reaction of a NIR-dye. This was followed by further reaction with a novel pre-activated form of the photosensitiser chlorin e6 with the exposed fragments' lysine residues. Specific binding of the theranostic agent was observed in vitro with a HER2 positive cell line and cellular near-infrared fluorescence was observed with flow cytometry. Specific photo-activity of the conjugates when exposed to laser light was observed with HER2 positive but not HER2 negative cell lines in vitro, this selectivity was not seen with the unconjugated drug. This theranostic agent demonstrates that two different photo-active functions can be coupled to the same antibody fragment with little interference to their independent activities

Discovering Conformational Sub-States Relevant to Protein Function

Background: Internal motions enable proteins to explore a range of conformations, even in the vicinity of native state. The role of conformational fluctuations in the designated function of a protein is widely debated. Emerging evidence suggests that sub-groups within the range of conformations (or sub-states) contain properties that may be functionally relevant. However, low populations in these sub-states and the transient nature of conformational transitions between these substates present significant challenges for their identification and characterization. Methods and Findings: To overcome these challenges we have developed a new computational technique, quasianharmonic analysis (QAA). QAA utilizes higher-order statistics of protein motions to identify sub-states in the conformational landscape. Further, the focus on anharmonicity allows identification of conformational fluctuations that enable transitions between sub-states. QAA applied to equilibrium simulations of human ubiquitin and T4 lysozyme reveals functionally relevant sub-states and protein motions involved in molecular recognition. In combination with a reaction pathway sampling method, QAA characterizes conformational sub-states associated with cis/trans peptidyl-prolyl isomerization catalyzed by the enzyme cyclophilin A. In these three proteins, QAA allows identification of conformational sub-states, with critical structural and dynamical features relevant to protein function. Conclusions: Overall, QAA provides a novel framework to intuitively understand the biophysical basis of conformational diversity and its relevance to protein function. © 2011 Ramanathan et al

Greek politicians and the use of online technologies for citizen engagement

\ua9 2014, IGI Global. This chapter examines how politicians utilise Internet technologies to create an online presence, the motivating and hindering factors for doing so, and the perceived significance such a presence can have. The authors present empirical data collected via in-depth interviews with Greek politicians taking part in the general elections of 2012. The findings suggest that although politicians are increasingly interested in engaging with citizens using online technologies, their efforts are not always focused on achieving measurable and tangible results. Consequently, they do not make full use of the potential online technologies offer. Instead, the authors conclude, online strategies need to be organised around predefined objectives and based on clear communication and engagement plans