The EU in Times of COVID-19: Together into a Future Based on (More) Solidarity?

The outbreak of the COVID-19-pandemic in spring 2020 put solidarity within the European Union to the test and called for far-reaching responses by all member states to mitigate the pandemic’s effects. But how does this crisis affect public support for transnational solidarity in the European Union? Are EU citizens willing to collectively overcome a financial crisis caused by the COVID-19-pandemic? And what is the public opinion on the establishment of a fictional EU-wide fund to mitigate future crises? Those questions have been addressed by a multi-country survey run by the ‘Solikris’-project. A resulting analysis in the project’s Policy Brief #5 shows that particularly the macroeconomic differences between countries correlate with attitudes towards solidarity policies and that transnational solidarity is higher in situations of acute crisis.Der Ausbruch der COVID-19-Pandemie im Frühjahr 2020 stellte die Solidarität innerhalb der Europäischen Union auf die Probe und erforderte weitreichende Reaktionen aller Mitgliedsstaaten, um die Auswirkungen der Pandemie zu mildern. Doch wie wirkt sich diese Krise auf die öffentliche Unterstützung für transnationale Solidarität in der Europäischen Union aus? Sind die EU-Bürger*innen bereit, eine durch die COVID-19-Pandemie verursachte Finanzkrise kollektiv zu bewältigen? Und wie ist die öffentliche Meinung zur Einrichtung eines fiktiven EU-weiten Fonds zur Abfederung zukünftiger Krisen? Diese Fragen wurden in einer länderübergreifenden Umfrage des ‘Solikris’-Projekts untersucht. Eine daraus resultierende Analyse im Policy Brief #5 des Projekts zeigt, dass insbesondere die makroökonomischen Unterschiede zwischen den Ländern mit den Einstellungen zur Solidaritätspolitik korrelieren und dass die transnationale Solidarität in akuten Krisensituationen höher is

Die EU in Zeiten von COVID-19: Gemeinsam in eine solidarische(re) Zukunft?

Die Covid-19-Pandemie stellt die europäische Gemeinschaft vor neue Herausforderungen. Sind EU-Bürger:innen gewillt eine durch COVID-19 entstandene Finanzkrise gemeinsam zu bewältigen und wie gestaltet sich das öffentliche Meinungsbild zur Einrichtung eines fiktiven EU-weiten Fonds für die Bekämpfung von zukünftigen Krisen? Die Ergebnisse einer Mehrländer-Befragung zeigen, dass die makroökonomischen Unterschiede der Länder die dominierende Konfliktdimension darstellen

Alternative splicing of coq-2 controls the levels of rhodoquinone in animals

Parasitic helminths use two benzoquinones as electron carriers in the electron transport chain. In normoxia, they use ubiquinone (UQ), but in anaerobic conditions inside the host, they require rhodoquinone (RQ) and greatly increase RQ levels. We previously showed the switch from UQ to RQ synthesis is driven by a change of substrates by the polyprenyltransferase COQ-2 (Del Borrello et al., 2019; Roberts Buceta et al., 2019); however, the mechanism of substrate selection is not known. Here, we show helminths synthesize two coq-2 splice forms, coq-2a and coq-2e, and the coq-2e-specific exon is only found in species that synthesize RQ. We show that in Caenorhabditis elegans COQ-2e is required for efficient RQ synthesis and survival in cyanide. Importantly, parasites switch from COQ-2a to COQ-2e as they transit into anaerobic environments. We conclude helminths switch from UQ to RQ synthesis principally via changes in the alternative splicing of coq-2.Agencia Nacional de Investigación e InnovaciónCanadian Institutes of Health Researc

Alternative splicing of coq-2 controls the levels of rhodoquinone in animals

Parasitic helminths use two benzoquinones as electron carriers in the electron transport chain. In normoxia, they use ubiquinone (UQ), but in anaerobic conditions inside the host, they require rhodoquinone (RQ) and greatly increase RQ levels. We previously showed the switch from UQ to RQ synthesis is driven by a change of substrates by the polyprenyltransferase COQ-2 (Del Borrello et al., 2019; Roberts Buceta et al., 2019); however, the mechanism of substrate selection is not known. Here, we show helminths synthesize two coq-2 splice forms, coq-2a and coq-2e, and the coq-2e-specific exon is only found in species that synthesize RQ. We show that in Caenorhabditis elegans COQ-2e is required for efficient RQ synthesis and survival in cyanide. Importantly, parasites switch from COQ-2a to COQ-2e as they transit into anaerobic environments. We conclude helminths switch from UQ to RQ synthesis principally via changes in the alternative splicing of coq-2.Agencia Nacional de Investigación e InnovaciónCanadian Institutes of Health Researc

Structure and nucleic acid binding properties of KOW domains 4 and 6–7 of human transcription elongation factor DSIF

The human transcription elongation factor DSIF is highly conserved throughout all kingdoms of life and plays multiple roles during transcription. DSIF is a heterodimer, consisting of Spt4 and Spt5 that interacts with RNA polymerase II (RNAP II). DSIF binds to the elongation complex and induces promoter-proximal pausing of RNAP II. Human Spt5 consists of a NusG N-terminal (NGN) domain motif, which is followed by several KOW domains. We determined the solution structures of the human Spt5 KOW4 and the C-terminal domain by nuclear magnetic resonance spectroscopy. In addition to the typical KOW fold, the solution structure of KOW4 revealed an N-terminal four-stranded β-sheet, previously designated as the KOW3-KOW4 linker. In solution, the C-terminus of Spt5 consists of two β-barrel folds typical for KOW domains, designated KOW6 and KOW7. We also analysed the nucleic acid and RNAP II binding properties of the KOW domains. KOW4 variants interacted with nucleic acids, preferentially single stranded RNA, whereas no nucleic acid binding could be detected for KOW6-7. Weak binding of KOW4 to the RNAP II stalk, which is comprised of Rpb4/7, was also detected, consistent with transient interactions between Spt5 and these RNAP II subunits