XYLAZINE-KETAMINE ANAESTHESIA; COMPARATIVE STUDIES IN MALE AND FEMALE CANE RATS (THRYONOMYS SWINDERIANUS)

Objective: Domestication of the Cane rats is on-going globally and might replace the conventional rodents used in biomedical research in future. However, the paucity of information on adequate anaesthesia vis-a-vis xylazine-ketamine drug combination in the male and female Cane rats warranted this study.Methods: Six adult Cane rats {1.8±0.7 kg body weights (bwt.)} assigned into group A (Female) and group B (Male) of three rats each was used for the study. Each animal was premedicated with atropine sulphate (0.05 mg/kg bwt), and later administered xylazine (10 mg/kg bwt) and ketamine (100 mg/kg bwt) intramuscularly. Meanwhile, anaesthetic characteristics and physiologic indices of anaesthesia were monitored.Results: Results obtained showed that the physiologic indices; open eyelids, smooth induction and recovery, skeletal muscle relaxation and somatic analgesia were observed in all the animals, however, the anaesthetic indices; time to induction, time to standing, duration of analgesia and duration of recumbency showed marked sex variations. The mean values for the duration of analgesia and recumbency were significantly elevated (P<0.05) in group B as compared with group A. Similar trend was seen for time to standing, but, it was non-significant (P>005). However, time to induction mean value for group A was non-significantly increased (P>005) when compared with group B. The heart rate, respiratory rate and rectal temperature mean values decreased in both groups non-significantly (P>0.05).Conclusion: Conclusively, the xylazine-ketamine combination produced anaesthesia in Cane rat and the combination is more tolerated in the male Cane rats than the female Cane rats

Training Neural networks for Experimental models: Classifying Biomedical Datasets for Sickle Cell Disease

This paper presents the use of various type of neural network architectures for the classification of medical data. Extensive research has indicated that neural networks generate significant improvements when used for the pre-processing of medical time-series data signals and have assisted in obtaining high accuracy in the classification of medical data. Up to date, most of hospitals and healthcare sectors in the United Kingdom are using manual approach for analysing patient input for sickle cell disease, which depends on clinician’s experience that can lead to time consuming and stress to patents. The results obtained from a range of models during our experiments have shown that the proposed Back-propagation trained feed-forward neural network classifier generated significantly better outcomes over the other range of classifiers. Using the ROC curve, experiments results showed the following outcomes for our models, in order of best to worst: Back-propagation trained feed-forward neural net classifier: 0.989, Functional Link neural Network: 0.972, in comparison to the Radial basis neural Network Classifiers with areas of 0.875, and the Voted Perception classifier: 0.766. A Linear Neural Network was used as baseline classifier to illustrate the importance of the previous models, producing an area of 0.849, followed by a random guessing model with an area of 0.524

Closing the diagnostic gap: Liquid biopsy potential to transform ovarian cancer outcomes in sub-Saharan Africa

Ovarian cancer presents a significant health challenge in sub-Saharan Africa (SSA), where late-stage diagnosis contributes to high mortality rates. This diagnostic gap arises from limited resources, poor healthcare infrastructure, and a lack of awareness about the disease. However, a potential game-changer is emerging in the form of liquid biopsy (LB), a minimally invasive diagnostic method. This paper analyses the current diagnostic gap in ovarian cancer in SSA, highlighting the socio-economic, cultural, and infrastructural factors that hinder early diagnosis and treatment. It discusses the challenges and potential of LB in the context of SSA, emphasizing its cost-effectiveness and adaptability to resource-limited settings. The transformative potential of LB in SSA is promising, offering a safer, more accessible, and cost-effective approach to ovarian cancer diagnosis. This paper provides recommendations for future directions, emphasizing the need for research, infrastructure development, stakeholder engagement, and international collaboration. By recognizing the transformative potential of LB and addressing the diagnostic gap, we can pave the way for early detection, improved treatment, and better outcomes for ovarian cancer patients in SSA. This paper sheds light on a path toward better healthcare access and equity in the region

Eco-friendly one-pot synthesis of Prussian blue-embedded magnetic hydrogel beads for the removal of cesium from water

A simple one-step approach to fabricating Prussian blue-embedded magnetic hydrogel beads (PBMHBs) was fabricated for the effective magnetic removal of radioactive cesium (Cs-137) from water. Through the simple dropwise addition of a mixed aqueous solution of iron salts, commercial PB and polyvinyl alcohol (PVA) to an ammonium hydroxide (NH4OH) solution, the formation of hydrogel beads and the encapsulation of PB in beads were achieved in one pot through the gelation of PVA with in situ-formed iron oxide nanoparticles as the cross-linker. The obtained PB-MHBs, with 43.77 weight %of PB, were stable without releasing PB for up to 2 weeks and could be effectively separated from aqueous solutions by an external magnetic field, which is convenient for the large-scale treatment of Cs-contaminated water. Detailed Cs adsorption studies revealed that the adsorption isotherms and kinetics could be effectively described by the Langmuir isotherm model and the pseudo-second-order model, respectively. Most importantly, the PB-MHBs exhibited excellent selectivity for Cs-137 in (137)Cscontaminated simulated groundwater (55 Bq/g) with a high removal efficiency (>99.5%), and the effective removal of Cs-137 from real seawater by these PB-MHBs demonstrated the excellent potential of this material for practical application in the decontamination of Cs-137-contaminated seawate

Community-based interventions to improve and sustain antiretroviral therapy adherence, retention in HIV care and clinical outcomes in low- and middle-income countries for achieving the UNAIDS 90-90-90 targets

Little is known about the effect of community versus health facility-based interventions to improve and sustain antiretroviral therapy (ART) adherence, virologic suppression, and retention in care among HIV-infected individuals in low- and middle-income countries (LMICs). We systematically searched four electronic databases for all available randomized controlled trials (RCTs) and comparative cohort studies in LMICs comparing community versus health facility-based interventions. Relative risks (RRs) for pre-defined adherence, treatment engagement (linkage and retention in care), and relevant clinical outcomes were pooled using random effect models. Eleven cohort studies and eleven RCTs (N = 97,657) were included. Meta-analysis of the included RCTs comparing community- versus health facility-based interventions found comparable outcomes in terms of ART adherence (RR = 1.02, 95 % CI 0.99 to 1.04), virologic suppression (RR = 1.00, 95 % CI 0.98 to 1.03), and all-cause mortality (RR = 0.93, 95 % CI 0.73 to 1.18). The result of pooled analysis from the RCTs (RR = 1.03, 95 % CI 1.01 to 1.06) and cohort studies (RR  = 1.09, 95 % CI 1.03 to 1.15) found that participants assigned to community-based interventions had statistically significantly higher rates of treatment engagement. Two studies found community-based ART delivery model either cost-saving or cost-effective. Community- versus facility-based models of ART delivery resulted in at least comparable outcomes for clinically stable HIV-infected patients on treatment in LMICs and are likely to be cost-effective

Negative emotional stimuli reduce contextual cueing but not response times in inefficient search

In visual search, previous work has shown that negative stimuli narrow the focus of attention and speed reaction times (RTs). This paper investigates these two effects by first asking whether negative emotional stimuli narrow the focus of attention to reduce the learning of a display context in a contextual cueing task and, second, whether exposure to negative stimuli also reduces RTs in inefficient search tasks. In Experiment 1, participants viewed either negative or neutral images (faces or scenes) prior to a contextual cueing task. In a typical contextual cueing experiment, RTs are reduced if displays are repeated across the experiment compared with novel displays that are not repeated. The results showed that a smaller contextual cueing effect was obtained after participants viewed negative stimuli than when they viewed neutral stimuli. However, in contrast to previous work, overall search RTs were not faster after viewing negative stimuli (Experiments 2 to 4). The findings are discussed in terms of the impact of emotional content on visual processing and the ability to use scene context to help facilitate search

Effects of acute and chronic cobalt treatment on adult rat cardiomyocyte calcium handling

Patients with cobalt-chromium hip arthroplasties display increased cobalt levels in circulation. Elevated cobalt has been linked to cardiomyopathy, yet the mechanisms underlying cobalt-induced pathology remain unknown. Here, we have examined the effects of acute ( Cardiomyocytes were isolated enzymatically from adult male rats (n=9) and treated acutely for 5min, 1h and 24h with 1, 10 and 100µM CoCl2. After loading with Cal520 AM, calcium transients were measured during electrical pacing at 1Hz and sparks were recorded using confocal microscopy. Chronic cobalt effects were measured in cardiac preparations taken from adult male rats (n=8) injected daily with CoCl2(1mg/kg) for 28 days. A concentration and time-dependent decrease in Ca2+ transient amplitude was evident in cobalt-treated cells compared to controls, with 10μM CoCl2 inducing an amplitude reduction of 15.1±5.4% and 30.0±7.2% at 5min and 1h respectively and 100µM resulting in 25.6±0.05% and 58.8±0.09% reduction at 5min and 1h. Spark frequency was increased with 100µM cobalt relative to control, resulting in 55.4±0.9% and 76.8±1.4% increase at 5min and 1h. RyR2 expression in chronic cobalt-treated ventricular tissue was similar to controls, suggesting RyR2 post-translational modification may account for altered spark frequency. This study demonstrates reduced Ca2+ release and increased RyR2 activation in cardiomyocytes treated acutely with CoCl2. Indications suggest RyR2 phosphorylation may be involved in the negative inotropic effects observed

Integration of VR with BIM to facilitate real-time creation of bill of quantities during the design phase:a proof of concept study

As time goes on and building practices change, procedures that at one point seemed indispensable can fall by the wayside. One such example is the bill of quantities (B/Q). Research into the extant literature attributes declining use of B/Qs to a multitude of reasons, such as its complexity and potentially drawn-out time to produce, non-traditional procurement systems growing in popularity and the challenge of using its information in a construction schedule. With these issues in mind, a combined process of Building Information Modelling (BIM), Virtual Reality (VR) and including the client in the design process has been proposed as a potential solution. Following a literature review and precedent study, an experiment was carried out using this new process to simulate a client’s design decisions on window and interior furnishings, specifically. Their choices made using VR automatically updated a B/Q Revit Schedule and allowed the client to have a firm grasp on the project costs. Not only did this process give the client more confidence in a pleasing final outcome, but the technology ensured an up-to-date, accurate and easily understood B/Q. Here lies great potential savings in cost, time and gives the B/Q a newfound importance in future construction processes. The research case presented in this paper was a stepping stone in exploring new opportunities offered by VR and BIM and how they could improve the reliability and accuracy of traditional procurement within construction, specifically within the B/Q document