Molecular characterization of Rhizobium strains isolated from wild chickpeas collected from high altitudes in Erzurum-Turkey

The fixation of N2 by legumes plays key role in agricultural sustainability. Moreover, the further assessment of rhizobial genetic diversity is contributing both to the worldwide knowledge of biodiversity of soil microorganisms and to the usefulness of rhizobial collections, and it is developing long-term strategies to increase contributions of legume-fixed to agricultural productivity. In the last decades, the use of molecular techniques has been contributed greatly to enhance the knowledge of rhizobial diversity. This study was conducted to determine the phenotypic and genotypic differences in Rhizobium leguminosarum subsp. ciceri strains isolated from perennial wild chickpeas (Cicer anatolicum) from high altitudes (2000-2500 m) in mountains of Erzurum, Eastern Anatolia, Turkey. In this study, rep-PCR (ERIC-, REP- and BOX-PCR) fingerprinting methods were used for the genotypic characterization and phylogenetic analysis of Rhizobium leguminosarum subsp. ciceri strains isolated from perennial wild chickpeas. The results showed a high intraspecies diversity among the strains in terms of rep-PCR (ERIC-, REP- and BOX-PCR) profiles. Copyright © 2009 Bucharest University

Synthesis of some novel isoxazolidine derivatives via 1,3-dipolar cycloaddition and their biological evaluation

A series of novel substituted isoxazolidine derivatives were synthesized by 1,3-dipolar cycloaddition reactions of a-aryl-N-methyl nitrones with diethyl maleate. The structures of the synthesized compounds were characterized using spectroscopic methods. All the synthesized compounds were screened for their antibacterial activities against Gram-positive and Gram-negative bacteria and for their antifungal activities against a yeast strain. The results show that all the synthesized compounds displayed significant activity against S. epidermidis, M. luteus, B. cereus, B. abortus and C. albicans when compared to standard drugs. © 2019, Gazi University Eti Mahallesi. All rights reserved

Synthesis of some novel isoxazolidine derivatives via 1,3-dipolar cycloaddition and their biological evaluation

A series of novel substituted isoxazolidine derivatives were synthesized by 1,3-dipolar cycloaddition reactions of a-aryl-N-methyl nitrones with diethyl maleate. The structures of the synthesized compounds were characterized using spectroscopic methods. All the synthesized compounds were screened for their antibacterial activities against Gram-positive and Gram-negative bacteria and for their antifungal activities against a yeast strain. The results show that all the synthesized compounds displayed significant activity against S. epidermidis, M. luteus, B. cereus, B. abortus and C. albicans when compared to standard drugs. © 2019, Gazi University Eti Mahallesi. All rights reserved

Synthesis and in-vitro antimicrobial and antimutagenic activities of some novel 2-(2-hydroxybenzylideneamino)-5,7-dihydro-4hthieno[ 2,3-c]pyran-3-carbonitrile derivatives

In this investigation, a series of some novel 2-(2-hydroxybenzylideneamino)-5,7-dihydro-4Hthieno[ 2,3-c]pyran-3-carbonitriles (4a-f) have been synthesized. These substances have all been examined for antibacterial activities against pathogenic strains Listeria monocytogenes 4b, Staphylococcus aureus, Escherichia coli, Salmonella typhi H, Pseudomonas putida, Brucella abortus, Shigella dysenteriae.Thype 7, Staphylococcus epidermis, Micrococcus luteus, Bacillus cereus, Enterobacter aerogenes and antifungal activity against Candida albicans. Most of the studied compounds were found effective against bacteria and yeast. Compound 4f exhibited activity against Pseudomonas putida, Brucella abortus, Staphylococcus aureus, Candida albicans and 4c Brucella abortus, Staphylococcus epidermis comparable to standard antibiotics. In addition, the anti-genotoxic activity of these substances was evaluated by a micronucleus (MN) test. Among the synthesized compounds, 4c was found to have the most anti-mutagenic effect against AFB1. It was also found that different concentrations of these substances suppressed the mutagenic effects of Aflatoxin B1 (AFB1) in the MN test (however 10 µM is the most effective dose). It seems that the antimutagenic effects of these substances may originate from their antioxidant potency or reaction of the aflatoxine lactone carbonyl functional group

Nanospheres caped Pt(II) and Pt (IV): synthesis and evaluation as antimicrobial and Antifungal Agent

Antimicrobial and antifungal polymers are gaining the attention of pharmaceutical makers and industrial design. Nanospheres-Polymers attached Platinum(II) / (IV) complexes have been synthesized to investigate antimicrobial activities. Firstly, nanospheres involving Schiff bases were synthesized from (aminomethyl) polystyrene and four substitute salicylaldehyde (2-hydroxy benzaldehyde, 5-fluoro-2-hydroxy benzaldehyde, 5-kloro-2-hydroxy benzaldehyde, 5-bromo-2-hydroxy benzaldehyde). Secondly, polymers attached Platinum(II) / (IV) complexes have been prepared by means of template method. The IR spectra show that the ligands act in a monovalent bidentate fashion all nanospheres involving Schiff bases. Square-planar and octahedral structures are proposed for Pt(II) and Pt(IV), respectively. All these substances have been examined for antibacterial activity against pathogenic strains, and antifungal activity. In particular, Pt(IV) complexes were more potent bactericides than all of the synthesized substances

Quantifying of bactericide properties of medicinal plants

Extended research has been carried out to clarify the ecological role of plant secondary metabolites (SMs). Although their primary ecological function is self-defense, bioactive compounds have long been used in alternative medicine or in biological control of pests. Several members of the family Labiatae are known to have strong antimicrobial capacity. For testing and quantifying antibacterial activity, most often standard microbial protocols are used, assessing inhibitory activity on a selected strain. In this study, the applicability of a microbial ecotoxtest was evaluated to quantify the aggregate bactericide capacity of Labiatae species, based on the bioluminescence inhibition of the bacterium Vibrio fischeri. Striking differences were found amongst herbs, reaching even 10-fold toxicity. Glechoma hederacea L. proved to be the most toxic, with the EC50 of 0.4073 g dried plant/l. LC50 values generated by the standard bioassay seem to be a good indicator of the bactericide property of herbs. Traditional use of the selected herbs shows a good correlation with bioactivity expressed as bioluminescence inhibition, leading to the conclusion that the Vibrio fischeri bioassay can be a good indicator of the overall antibacterial capacity of herbs, at least on a screening level