Development of biomimetic catalytic oxidation methods and non-salt methods using transition metal-based acid and base ambiphilic catalysts

This review focuses on the development of ruthenium and flavin catalysts for environmentally benign oxidation reactions based on mimicking the functions of cytochrome P-450 and flavoenzymes, and low valent transition-metal catalysts that replace conventional acids and bases. Several new concepts and new types of catalytic reactions based on these concepts are described

Global water scarcity including surface water quality and expansions of clean water technologies

Water scarcity threatens people in various regions, and has predominantly been studied from a water quantity perspective only. Here we show that global water scarcity is driven by both water quantity and water quality issues, and quantify expansions in clean water technologies (i.e. desalination and treated wastewater reuse) to ‘reduce the number of people suffering from water scarcity’ as urgently required by UN’s Sustainable Development Goal 6. Including water quality (i.e. water temperature, salinity, organic pollution and nutrients) contributes to an increase in percentage of world’s population currently suffering from severe water scarcity from an annual average of 30% (22%–35% monthly range; water quantity only) to 40% (31%–46%; both water quantity and quality). Water quality impacts are in particular high in severe water scarcity regions, such as in eastern China and India. In these regions, excessive sectoral water withdrawals do not only contribute to water scarcity from a water quantity perspective, but polluted return flows degrade water quality, exacerbating water scarcity. We show that expanding desalination (from 2.9 to 13.6 billion m3 month−1) and treated wastewater uses (from 1.6 to 4.0 billion m3 month−1) can strongly reduce water scarcity levels and the number of people affected, especially in Asia, although the side effects (e.g. brine, energy demand, economic costs) must be considered. The presented results have potential for follow-up integrated analyses accounting for technical and economic constraints of expanding desalination and treated wastewater reuse across the world

Intercomparison of global river discharge simulations focusing on dam operation --- Part II: Multiple models analysis in two case-study river basins, Missouri-Mississippi and Green-Colorado

We performed a twofold intercomparison of river discharge regulated by dams under multiple meteorological forcings among multiple global hydrological models for a historical period by simulation. Paper II provides an intercomparison of river discharge simulated by five hydrological models under four meteorological forcings. This is the first global multimodel intercomparison study on dam-regulated river flow. Although the simulations were conducted globally, the Missouri-Mississippi and Green-Colorado Rivers were chosen as case-study sites in this study. The hydrological models incorporate generic schemes of dam operation, not specific to a certain dam. We examined river discharge on a longitudinal section of river channels to investigate the effects of dams on simulated discharge, especially at the seasonal time scale. We found that the magnitude of dam regulation differed considerably among the hydrological models. The difference was attributable not only to dam operation schemes but also to the magnitude of simulated river discharge flowing into dams. That is, although a similar algorithm of dam operation schemes was incorporated in different hydrological models, the magnitude of dam regulation substantially differed among the models. Intermodel discrepancies tended to decrease toward the lower reaches of these river basins, which means model dependence is less significant toward lower reaches. These case-study results imply that, intermodel comparisons of river discharge should be made at different locations along the river’s course to critically examine the performance of hydrological models because the performance can vary with the locations

Insertion and Substitution Chemistry at the Boron Fourth Position in Charge-Neutral Zwitterionic Tripodal Tris(methimazolyl)borate Ligands

A number of new charge-neutral zwitterionic tris(methimazolyl)borate ligands have been synthesized, either by substitution of the dimethylamine group in the adduct (dimethylamine)tris(methimazolyl)borane (1) or by insertion into its B–N(dimethylamine) bond by an unsaturated Lewis base. Two new anionic ligands, (thiocyanato)tris(methimazolyl)borate and (cyano)tris(methimazolyl)borate, have also been accessed by this method

Tumor Marker Levels Before and After Curative Treatment of Hepatocellular Carcinoma as Predictors of Patient Survival.

BACKGROUND: α-fetoprotein (AFP) is used as a marker for hepatocellular carcinoma (HCC), which is influenced by hepatitis. Protein-induced vitamin K absence or antagonist II (PIVKA-II) is a sensitive diagnostic marker. Changes in these markers after treatment may reflect curability and predict outcome. METHODS: We conducted an analysis of prognosis in 470 HCC patients who received curative treatments, and examined the relationship between changes in AFP and PIVKA-II levels after 1 month of treatment in 156 patients. Subjects were divided into three groups according to changes in both levels: (1) normal (L) group before treatment, (2) normalization (N) or (3) decreased but still above normal level or unchanged (ANU) group after treatment. RESULTS: High AFP and PIVKA-II levels were significantly associated with poor tumor-free and overall survival. The presence of large size and advanced stage were significantly associated with prevalence of DU group. Overall survival in the AFP-L group was significantly better than that of other groups and overall survival in PIVKA-II-L and N groups were significantly better than that of the PIVKA-II-ANU groups. The combination of changes in the AFP- ANU and PIVKA-II- ANU groups showed the worst tumor-free and overall survivals. Multivariate analysis identified high pre-treatment levels of AFP and PIVKA-II and combination of AFP- ANU and PIVKA-II- ANU as significant determinants of poor tumor-free and overall survival, particularly in patients who underwent hepatectomy. CONCLUSION: We conclude that high levels of AFP or PIVKA-II after treatment for HCC did not sufficiently reflect curative efficacy of treatment and reflected a poor predictor of prognosis in HCC patients

Global water scarcity including surface water quality and expansions of clean water technologies

Water scarcity threatens people in various regions, and has predominantly been studied from a water quantity perspective only. Here we show that global water scarcity is driven by both water quantity and water quality issues, and quantify expansions in clean water technologies (i.e. desalination and treated wastewater reuse) to 'reduce the number of people suffering from water scarcity' as urgently required by UN's Sustainable Development Goal 6. Including water quality (i.e. water temperature, salinity, organic pollution and nutrients) contributes to an increase in percentage of world's population currently suffering from severe water scarcity from an annual average of 30% (22%–35% monthly range; water quantity only) to 40% (31%–46%; both water quantity and quality). Water quality impacts are in particular high in severe water scarcity regions, such as in eastern China and India. In these regions, excessive sectoral water withdrawals do not only contribute to water scarcity from a water quantity perspective, but polluted return flows degrade water quality, exacerbating water scarcity. We show that expanding desalination (from 2.9 to 13.6 billion m3 month−1) and treated wastewater uses (from 1.6 to 4.0 billion m3 month−1) can strongly reduce water scarcity levels and the number of people affected, especially in Asia, although the side effects (e.g. brine, energy demand, economic costs) must be considered. The presented results have potential for follow-up integrated analyses accounting for technical and economic constraints of expanding desalination and treated wastewater reuse across the world

Global water scarcity including surface water quality and expansions of clean water technologies

Water scarcity threatens people in various regions, and has predominantly been studied from a water quantity perspective only. Here we show that global water scarcity is driven by both water quantity and water quality issues, and quantify expansions in clean water technologies (i.e. desalination and treated wastewater reuse) to 'reduce the number of people suffering from water scarcity' as urgently required by UN's Sustainable Development Goal 6. Including water quality (i.e. water temperature, salinity, organic pollution and nutrients) contributes to an increase in percentage of world's population currently suffering from severe water scarcity from an annual average of 30% (22%–35% monthly range; water quantity only) to 40% (31%–46%; both water quantity and quality). Water quality impacts are in particular high in severe water scarcity regions, such as in eastern China and India. In these regions, excessive sectoral water withdrawals do not only contribute to water scarcity from a water quantity perspective, but polluted return flows degrade water quality, exacerbating water scarcity. We show that expanding desalination (from 2.9 to 13.6 billion m3 month−1) and treated wastewater uses (from 1.6 to 4.0 billion m3 month−1) can strongly reduce water scarcity levels and the number of people affected, especially in Asia, although the side effects (e.g. brine, energy demand, economic costs) must be considered. The presented results have potential for follow-up integrated analyses accounting for technical and economic constraints of expanding desalination and treated wastewater reuse across the world

Determination of cellularly processed HLA-A2402-restricted novel CTL epitopes derived from two cancer germ line genes, MAGE-A4 and SAGE

Purpose: For identification of CTL epitopes useful for cancer vaccines, it is crucial to determine whether cognate epitopes are presented on the cell surface of target cancer cells through natural processing of endogenous proteins. For this purpose, we tried to use the cellular machinery of both mice and human to define naturally processed CTL epitopes derived from two "cancer germ line" genes, MAGE-A4 and SAGE. Experimental Design: We vaccinated newly produced HLA-A2402 transgenic mice with DNA plasmids encoding target antigens. Following screening of synthesized peptides by splenic CD8(+) T cells of vaccinated mice, we selected candidate epitopes bound to HLA-A2402. We then examined whether human CD8(+) T cells sensitized with autologous CD4(+) PHA blasts transduced by mRNA for the cognate antigens could react with these selected peptides in an HLA-A2402-restricted manner. Results: After DNA vaccination, murine CD8(+) T cells recognizing MAGE-A4(143-15), or SAGE(715-723) in an HLA-A2402-restricted manner became detectable. Human CTLs specific for these two peptides were generated after sensitization of HLA-A2402- positive CD8(+) T cells with autologous CD4(+) PHA blasts transduced with respective mRNA. CTL clones were cytotoxic toward tumor cell lines expressing HLA-A2402 and cognate genes. Taken together, these CTL epitopes defined in HLA-A24 transgenic mice are also processed and expressed with HLA-A2402 in human cells. The presence of SAGE(715-723)-specific precursors was observed in HLA-A2402- positive healthy individuals. Conclusions: Two novel HLA-A2402- restricted CTL epitopes, MAGE-A4(143-15), and SAGE(715-723), were identified. Our approach assisted by cellular machinery of both mice and human could be widely applicable to identify naturally processed CTL epitopes