, sequence of the human CCK2R promoter −130 to −190 region, showing consensus -regulatory elements

<p><b>Copyright information:</b></p><p>Taken from "Regulation of mammalian gastrin/CCK receptor (CCK2R) expression and "</p><p></p><p>Experimental Physiology 2007;93(2):223-236.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2253704.</p><p>© The Authors. Journal compilation © 2007 The Physiological Society</p> Numbering is relative to the start of transcription (+1), which is taken as 193 bp upstream of the start of translation (see main text). , basal activity of CCK2R promoter–reporter constructs (size as indicated) 24 h after transfection in AGS cells. * Significantly reduced relative to 1700 bp construct ( < 0.001, = 3, ANOVA). , basal activity of CCK2R promoter–reporter constructs (size as indicated) 24 h after transfection in RGM1 cells. * Significantly reduced relative to 928 bp construct ( < 0.001, = 3, ANOVA). , basal activity of mutated 196 bp constructs 24 h after transfection into AGS cells. Mutations as indicated were made corresponding to the -regulatory elements underlined in . Values are percentage of wild-type (WT) sequence activity (= 100%). * Significantly reduced compared with WT activity ( < 0.001, = 3). , basal activity of mutated 196 bp constructs 24 h after transfection into RGM1 cells. Mutations as described in . Values are percentage of WT sequence activity (= 100%). * Significantly reduced compared with WT activity ( < 0.001, = 3, ANOVA)

, endogenous CCK2R mRNA abundance in AR42J cells in response to serum withdrawal for times indicated

<p><b>Copyright information:</b></p><p>Taken from "Regulation of mammalian gastrin/CCK receptor (CCK2R) expression and "</p><p></p><p>Experimental Physiology 2007;93(2):223-236.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2253704.</p><p>© The Authors. Journal compilation © 2007 The Physiological Society</p> Results are normalized to 18S rRNA and are expressed as percentage of full serum value (= 100%). = 4. * = 0.016, ** = 0.013, *** = 0.005 time 0, ANOVA. , CCK2R mRNA abundance in RGM1 cells in response to serum withdrawal for times indicated. Results are normalized to 18S rRNA and are expressed as percentage of full serum value (= 100%). = 3. * = 0.0016 time 0, ANOVA

, CCK2R immunoreactivity and DAPI staining in full-thickness corpus 2, 6, 9 and 13 days following cryoulcer generation

<p><b>Copyright information:</b></p><p>Taken from "Regulation of mammalian gastrin/CCK receptor (CCK2R) expression and "</p><p></p><p>Experimental Physiology 2007;93(2):223-236.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2253704.</p><p>© The Authors. Journal compilation © 2007 The Physiological Society</p> Note intense epithelial staining at ulcer repair margins at 13 days, and staining in the submucosa beneath the ulcer crater (indicated by white line) at 9 days. Scale bar represents 500 μ. Sections are representative from four individual animals at each time point. , CCK2R and smooth muscle α-actin immunoreactivity in gastric corpus epithelium at the repair margin of a 9 day ulcer. Upper panel, CCK2R (FITC); centre panel, smooth muscle α-actin (Texas Red); and lower panel, overlay. , CCK2R and vimentin immunoreactivity in gastric corpus submucosa beneath the repairing crater of a 9 day ulcer. Upper panel, CCK2R (FITC); centre panel, vimentin (Texas Red); and lower panel, overlay. , deconvolved images of gastric corpus epithelial cells at the repair margin of a 9 day ulcer. Upper left panel, CCK2R (FITC); upper right panel, smooth muscle α-actin (Texas Red); and lower panel, overlay. , deconvolved images of gastric corpus submucosal cells beneath the repairing crater of a 9 day ulcer. Upper panel, CCK2R (FITC); centre panel, vimentin (Texas Red); and lower panel, overlay. For and , scale bar represents 50 μm; for and , scale bar respresents 10 μm

, CCK2R expression in mucosa and submucosa 9 days following cryoulcer generation

<p><b>Copyright information:</b></p><p>Taken from "Regulation of mammalian gastrin/CCK receptor (CCK2R) expression and "</p><p></p><p>Experimental Physiology 2007;93(2):223-236.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2253704.</p><p>© The Authors. Journal compilation © 2007 The Physiological Society</p> Bracket indicates residual ulcer crater. , CCK2R expression in mucosa and submucosa 9 days following cryoulceration, 0.5 cm away from the ulcer site. C, regenerative mucosa, adjacent to cryoulcer. , submucosa below residual ulcer crater. , normal mucosa, 0.5 cm away from site of ulcer. , normal submucosa, 0.5 cm away from site of ulcer. For and , scale bar respresents 300 μm; for , scale bar represents 50 μm. Hybridizations were performed using a mixture (1:1:1) of the three oligonucleotides described in the Methods