, sequence of the human CCK2R promoter −130 to −190 region, showing consensus -regulatory elements

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Regulation of mammalian gastrin/CCK receptor (CCK2R) expression and "</p><p></p><p>Experimental Physiology 2007;93(2):223-236.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2253704.</p><p>© The Authors. Journal compilation © 2007 The Physiological Society</p> Numbering is relative to the start of transcription (+1), which is taken as 193 bp upstream of the start of translation (see main text). , basal activity of CCK2R promoter–reporter constructs (size as indicated) 24 h after transfection in AGS cells. * Significantly reduced relative to 1700 bp construct ( < 0.001, = 3, ANOVA). , basal activity of CCK2R promoter–reporter constructs (size as indicated) 24 h after transfection in RGM1 cells. * Significantly reduced relative to 928 bp construct ( < 0.001, = 3, ANOVA). , basal activity of mutated 196 bp constructs 24 h after transfection into AGS cells. Mutations as indicated were made corresponding to the -regulatory elements underlined in . Values are percentage of wild-type (WT) sequence activity (= 100%). * Significantly reduced compared with WT activity ( < 0.001, = 3). , basal activity of mutated 196 bp constructs 24 h after transfection into RGM1 cells. Mutations as described in . Values are percentage of WT sequence activity (= 100%). * Significantly reduced compared with WT activity ( < 0.001, = 3, ANOVA)