A genetic assessment of parentage in the blackspot sergeant damselfish, Abudefduf sordidus (Pisces: Pomacentridae)

Microsatellite markers were used to investigate the reproductive behavior of the damselfish Abudefduf sordidus at Johnston Atoll, Central Pacific Ocean. Genetic results indicated that ten males maintained guardianship over their nest territories for up to nine nest cycles during a 3.5 month period. Genotypes of 1025 offspring sampled from 68 nests (composed of 129 clutches) were consistent with 95% of the offspring being sired by the guardian male. Offspring lacking paternal alleles at two or more loci were found in 19 clutches, indicating that reproductive parasitism and subsequent alloparental care occurred. Reconstructed maternal genotypes allowed the identification of a minimum of 74 different females that spawned with these ten territorial males. Males were polygynous, mating with multiple females within and between cycles. Genetic data from nests, which consisted of up to four clutches during a reproductive cycle, indicated that each clutch usually had only one maternal contributor and that different clutches each had different dams. Females displayed sequential polyandry spawning with one male within a cycle but switched males in subsequent spawning cycles. These results highlight new findings regarding male parasitic spawning, polygyny, and sequential polyandry in a marine fish with exclusive male paternal care.Published versio

Pat1 RNA-binding proteins: Multitasking shuttling proteins.

Post-transcriptional regulation of gene expression is largely achieved at the level of splicing in the nucleus, and translation and mRNA decay in the cytosol. While the regulation may be global, through the direct inhibition of central factors, such as the spliceosome, translation initiation factors and mRNA decay enzymes, in many instances transcripts bearing specific sequences or particular features are regulated by RNA-binding factors which mobilize or impede recruitment of these machineries. This review focuses on the Pat1 family of RNA-binding proteins, conserved from yeast to man, that enhance the removal of the 5' cap by the decapping enzyme Dcp1/2, leading to mRNA decay and also have roles in translational repression. Like Dcp1/2, other decapping coactivators, including DDX6 and Edc3, and translational repressor proteins, Pat1 proteins are enriched in cytoplasmic P-bodies, which have a principal role in mRNA storage. They also concentrate in nuclear Cajal-bodies and splicing speckles and in man, impact splice site choice in some pre-mRNAs. Pivotal to these functions is the association of Pat1 proteins with distinct heptameric Lsm complexes: the cytosolic Pat1/Lsm1-7 complex mediates mRNA decay and the nuclear Pat1/Lsm2-8 complex alternative splicing. This dual role of human Pat1b illustrates the power of paralogous complexes to impact distinct processes in separate compartments. The review highlights our recent findings that Pat1b mediates the decay of AU-rich mRNAs, which are particularly enriched in P-bodies, unlike the decapping activator DDX6, which acts on GC-rich mRNAs, that tend to be excluded from P-bodies, and discuss the implications for mRNA decay pathways. This article is categorized under: RNA Turnover and Surveillance > Regulation of RNA Stability RNRNA Processing > Splicing Regulation/Alternative Splicing Translation > Translation Regulation.This work was supported by the BBSRC, Newton Trust and Foundation Wiener – Anspach (C.V.) to N.S.’s laboratory, and the Association pour la Recherche sur le Cancer and the Agence Nationale pour la Recherche contract ANR-14-CE09-0013-01 to D.W.’s laboratory

The yellow hypergiants HR 8752 and rho Cassiopeiae near the evolutionary border of instability

High-resolution near-ultraviolet spectra of the yellow hypergiants HR 8752 and rho Cassiopeiae indicate high effective temperatures placing both stars near the T_eff border of the ``yellow evolutionary void''. At present, the temperature of HR 8752 is higher than ever. For this star we found Teff=7900+-200 K, whereas rho Cassiopeiae has Teff=7300+-200 K. Both, HR 8752 and rho Cassiopeiae have developed strong stellar winds with Vinf ~ 120 km/s and Vinf ~ 100 km/s, respectively. For HR 8752 we estimate an upper limit for the spherically symmetric mass-loss of 6.7X10^{-6}M_solar/yr. Over the past decades two yellow hypergiants appear to have approached an evolutionary phase, which has never been observed before. We present the first spectroscopic evidence of the blueward motion of a cool super/hypergiant on the HR diagram.Comment: 13 pages including 3 figures. Accepted for publication in ApJ Letter

On the Dynamic Stability of Cool Supergiant Atmospheres

We have developed a new formalism to compute the thermodynamic coefficient Gamma1 in the theory of stellar and atmospheric stability. We generalize the classical derivation of the first adiabatic index, which is based on the assumption of thermal ionization and equilibrium between gas and radiation temperature, towards an expression which incorporates photo-ionization due to radiation with a temperature T_rad different from the local kinetic gas temperature.Our formalism considers the important non-LTE conditions in the extended atmospheres of supergiant stars. An application to the Kurucz grid of cool supergiant atmospheres demonstrates that models with T_rad =~ T_eff between 6500 K and 7500 K become most unstable against dynamic perturbations, according to Ledoux' stability integral . This results from Gamma1 and acquiring very low values, below 4/3, throughout the entire stellar atmosphere, which causes very high gas compression ratios around these effective temperatures. Based on detailed NLTE-calculations, we discuss atmospheric instability of pulsating massive yellow supergiants, like the hypergiant rho Cas (Ia+), which exist in the extension of the Cepheid instability strip, near the Eddington luminosity limit.Comment: 54 pages including figures and the Appendix, 7 figures, Accepted for The Astrophysical Journal, Main Journal, 558, Sept. 200

Imaging the dynamical atmosphere of the red supergiant Betelgeuse in the CO first overtone lines with VLTI/AMBER

We present the first 1-D aperture synthesis imaging of the red supergiant Betelgeuse in the individual CO first overtone lines with VLTI/AMBER. The reconstructed 1-D projection images reveal that the star appears differently in the blue wing, line center, and red wing of the individual CO lines. The 1-D projection images in the blue wing and line center show a pronounced, asymmetrically extended component up to ~1.3 stellar radii, while those in the red wing do not show such a component. The observed 1-D projection images in the lines can be reasonably explained by a model in which the CO gas within a region more than half as large as the stellar size is moving slightly outward with 0--5 km s^-1, while the gas in the remaining region is infalling fast with 20--30 km s^-1. A comparison between the CO line AMBER data taken in 2008 and 2009 shows a significant time variation in the dynamics of the CO line-forming region in the photosphere and the outer atmosphere. In contrast to the line data, the reconstructed 1-D projection images in the continuum show only a slight deviation from a uniform disk or limb-darkened disk. We derive a uniform-disk diameter of 42.05 +/- 0.05 mas and a power-law-type limb-darkened disk diameter of 42.49 +/- 0.06 mas and a limb-darkening parameter of (9.7 +/- 0.5) x 10^{-2}. This latter angular diameter leads to an effective temperature of 3690 +/- 54 K for the continuum-forming layer. These diameters confirm that the near-IR size of Betelgeuse was nearly constant over the last 18 years, in marked contrast to the recently reported noticeable decrease in the mid-IR size. The continuum data taken in 2008 and 2009 reveal no or only marginal time variations, much smaller than the maximum variation predicted by the current 3-D convection simulations.Comment: 21 pages, 12 figures, accepted for publication in Astronomy and Astrophysic