Primers and Probe to Identify Mycobacterium Tuberculosis Complex

Methods and nucleic acids for rapid, reliable and sensitive detection of Mycobacterium tuberculosis (MTB) complex pathogen in a biological sample. Oligonucleotides are provided which amplify MTB DNA and which are useful in carrying out real time PCR of DNA obtained from formalin-fixed and paraffin-embedded tissue samples

Computation of optimal singular controls

Computation of optimal singular control

Qualitative application of a result in control theory to problems of economic growth

Qualitative application of result in control theory to problem of economic growt

Release of β-galactosidase by Permeabilization of Indigenously Isolated Lactobacillus acidophilus Using Lysozyme

Enzymatic lysis, using lysozyme, has been shown in this paper to be an effective cell lysis method to release β-galactosidase from Lactobacillus acidophilus indigenously isolated from Eleusine coracana. The lytic process was developed using response surface methodology (RSM) to optimize lysozyme concentration, cell density and incubation time critical for the release of β-galactosidase. The optimized permeabilization condition (lysozyme: 33.63 · 104 U mL–1; cell density: 4.7 % w/v on wet basis; incubation time: 10h 30min.) resulted in 1.2 fold increase in release of β-galactosidase when compared with an optimized ultrasonication process. These optimized conditions were used to determine the release constants of β-galactosidase and total protein release as a function of temperature. The enzyme and protein release constants were used further to calculate the location factor of β-galactosidase

Release of β-galactosidase by Permeabilization of Indigenously Isolated Lactobacillus acidophilus Using Lysozyme

Enzymatic lysis, using lysozyme, has been shown in this paper to be an effective cell lysis method to release β-galactosidase from Lactobacillus acidophilus indigenously isolated from Eleusine coracana. The lytic process was developed using response surface methodology (RSM) to optimize lysozyme concentration, cell density and incubation time critical for the release of β-galactosidase. The optimized permeabilization condition (lysozyme: 33.63 · 104 U mL–1; cell density: 4.7 % w/v on wet basis; incubation time: 10h 30min.) resulted in 1.2 fold increase in release of β-galactosidase when compared with an optimized ultrasonication process. These optimized conditions were used to determine the release constants of β-galactosidase and total protein release as a function of temperature. The enzyme and protein release constants were used further to calculate the location factor of β-galactosidase

Synchronization of organ pipes: experimental observations and modeling

We report measurements on the synchronization properties of organ pipes. First, we investigate influence of an external acoustical signal from a loudspeaker on the sound of an organ pipe. Second, the mutual influence of two pipes with different pitch is analyzed. In analogy to the externally driven, or mutually coupled self-sustained oscillators, one observes a frequency locking, which can be explained by synchronization theory. Further, we measure the dependence of the frequency of the signals emitted by two mutually detuned pipes with varying distance between the pipes. The spectrum shows a broad ``hump'' structure, not found for coupled oscillators. This indicates a complex coupling of the two organ pipes leading to nonlinear beat phenomena.Comment: 24 pages, 10 Figures, fully revised, 4 big figures separate in jpeg format. accepted for Journal of the Acoustical Society of Americ

Design space exploration for providing QoS within the HARMONY framework

ABSTRACT The HARMONY architectur

Unstructured Large Eddy Simulation Technology for Prediction and Control of Jet Noise

Development of concepts for reduction of jet noise has relied heavily on expensive experimental testing of various nozzle designs. For example, the design of nozzle serrations (chevron) and internal mixer/ejector nozzles have relied largely on laboratory and full-scale testing. Without a deeper understanding of the sources of high-speed jet noise it is very difficult to effectively design configurations that reduce the noise and maintain other performance metrics such as nozzle thrust. In addition, the high complexity of the flow limits the success of a parametric black-box optimization

A two-species continuum model for aeolian sand ripples

We formulate a continuum model for aeolian sand ripples consisting of two species of grains: a lower layer of relatively immobile clusters, with an upper layer of highly mobile grains moving on top. We predict analytically the ripple wavelength, initial ripple growth rate and threshold saltation flux for ripple formation. Numerical simulations show the evolution of realistic ripple profiles from initial surface roughness via ripple growth and merger.Comment: 9 pages, 3 figure

Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphe neurones and cranial motoneurones

In this study, we elucidate the roles of the winged-helix transcription factor Foxa2 in ventral CNS development in zebrafish. Through cloning of monorail (mol), which we find encodes the transcription factor Foxa2, and phenotypic analysis of mol(-/-) embryos, we show that floorplate is induced in the absence of Foxa2 function but fails to further differentiate. In mol(-/-) mutants, expression of Foxa and Hh family genes is not maintained in floorplate cells and lateral expansion of the floorplate fails to occur. Our results suggest that this is due to defects both in the regulation of Hh activity in medial floorplate cells as well as cell-autonomous requirements for Foxa2 in the prospective laterally positioned floorplate cells themselves. Foxa2 is also required for induction and/or patterning of several distinct cell types in the ventral CNS. Serotonergic neurones of the raphe nucleus and the trochlear motor nucleus are absent in mol(-/-) embryos, and oculomotor and facial motoneurones ectopically occupy ventral CNS midline positions in the midbrain and hindbrain. There is also a severe reduction of prospective oligodendrocytes in the midbrain and hindbrain. Finally, in the absence of Foxa2, at least two likely Hh pathway target genes are ectopically expressed in more dorsal regions of the midbrain and hindbrain ventricular neuroepithelium, raising the possibility that Foxa2 activity may normally be required to limit the range of action of secreted Hh proteins