Detection of IL28B SNP DNA from Buccal Epithelial Cells, Small Amounts of Serum, and Dried Blood Spots

Background & Aims: Point mutations in the coding region of the interleukin 28 gene (rs12979860) have recently been identified for predicting the outcome of treatment of hepatitis C virus infection. This polymorphism detection was based on whole blood DNA extraction. Alternatively, DNA for genetic diagnosis has been derived from buccal epithelial cells (BEC), dried blood spots (DBS), and genomic DNA from serum. The aim of the study was to investigate the reliability and accuracy of alternative routes of testing for single nucleotide polymorphism allele rs12979860CC. Methods: Blood, plasma, and sera samples from 200 patients were extracted (400 mL). Buccal smears were tested using an FTA card. To simulate postal delay, we tested the influence of storage at ambient temperature on the different sources of DNA at five time points (baseline, 48 h, 6 days, 9 days, and 12 days) Results: There was 100 % concordance between blood, plasma, sera, and BEC, validating the use of DNA extracted from BEC collected on cytology brushes for genetic testing. Genetic variations in HPTR1 gene were detected using smear technique in blood smear (3620 copies) as well as in buccal smears (5870 copies). These results are similar to those for whole blood diluted at 1/10. A minimum of 0.04 mL, 4 mL, and 40 mL was necessary to obtain exploitable results respectively for whole blood, sera, and plasma. No significant variation between each time point was observed for the different sources of DNA. IL28B SNPs analysis at these different time points showed the same results using the four sources of DNA

Nucleophilic or Electrophilic Interactions of C2 with HX systems (X=F, Cl, Br)

8International audienceHighly correlated ab-initio wavefunctions within the MRCI approach are used in a comparative study of the interactions between C2 and the three hydrogen halides HX (X = F, Cl, Br). Test calculations are also presented using the UCCSD(T)-F12 approach. The asymptotic regions are investigated for different relative orientations of the two moieties. It is shown that the three systems C2 + HX are bound, for intermolecular distances close to 3 Ang, through nucleophilic interactions between C2 and HX for approaches perpendicular to the C-C axis, with decreasing interaction energies from HF to HBr. For HX approaching C2 along its axis, the interactions, governed by the electrophilic character of C2 are decreasing from HBr to HF. Even though the reactions towards the molecular systems HCCX or CCHX are exothermic, activation barriers (0.58 eV and more) are calculated at short distances, preventing the direct reactions towards the corresponding tetra-atomic system

Are there Stable Excited Triplet States of NCS-/CNS- and NCO-/CNO-?

International audienceHighly correlated ab initio wave functions within the UCCSD(T)-F12 approach have been used to map portions of the potential energy surfaces (PESs) and to study the stability of the first excited triplet states of the NCS-/CNS- and NCO-/CNO- anions. These a3Π states for linear geometries, or their 3A' and 3A" bent components, correlate with the lowest dissociation asymptote of NCX- (X = S and O) along the NC-X coordinates. The X1Σ+ linear ground states of these anions are known to be stable with respect to dissociation to the X2Π ground state of the corresponding neutral molecule with a rather large electron affinity (EA). The a3Π state of the NCS- anion is positioned below the X state of the neutral at long NC-S distances and its minimum of energy is found for bent geometries. The stability of its two components in bent geometries has been investigated, and it is found that some anionic forms are stable with respect to the X state of the neutral. The linear CNS- and CNO- isomers present a minimum only at long CN-X distances, located below the minimum of their corresponding neutral CNX ground states

Nucleophilic or Electrophilic Interactions of C₂ with HX Systems (X = F, Cl, Br)

Highly correlated <i>ab initio</i> wave functions within the MRCI approach are used in a comparative study of the interactions between C₂ and the three hydrogen halides HX (X = F, Cl, Br). Test calculations are also presented using the UCCSD­(T)-F12 approach. The asymptotic regions are investigated for different relative orientations of the two moieties. It is shown that the three systems C₂ + HX are bound, for intermolecular distances close to 3 Å, through nucleophilic interactions between C₂ and HX for approaches perpendicular to the C–C axis, with decreasing interaction energies from HF to HBr. For HX approaching C₂ along its axis, the interactions, governed by the electrophilic character of C₂ are decreasing from HBr to HF. Even though the reactions toward the molecular systems HCCX or CCHX are exothermic, activation barriers (0.58 eV and more) are calculated at short distances, preventing the direct reactions toward the corresponding tetra-atomic systems

A proactive approach to assess rising STIs among different at-risk groups of MSM in the early era of PrEP: a real-world clinical care setting

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