The effects of the material properties of copper-tungsten and silver-tungsten composites on the electrical discharge machining wear ratio

Eight compositions of copper-tungsten composites, eight compositions of silver-tungsten composites, as well as copper, silver and tungsten tool electrodes were used to machine copper and molybdenum workpieces: the objective of the investigation was to define the wear ratio of these materials in terms of cohesive energy and electrical conductivity

The Role of Service-Learning and Mentoring in the Early Career Development of a Research Methodologist

Anxiety about statistics may impede new scholars from developing quantitative research skills and from sharing those skills in service-learning, internship, and work settings. Using an interpretive case study design with a convenience sample of one emerging student leader in a collaborative university-community service-learning research project, we explored the question “How did the career path of a quantitatively skilled researcher develop?” Data collected over a 3-and-a-half-year period included 7 semi-structured interviews with the student during her master’s and doctoral program and interviews with 3 mentors, 2 peers, and 2 community partners, as well as observations and documents. A constant comparison analysis method identified emerging themes: the role of mentors in building skills, building trust, and modeling risk taking. The results suggest strategies for increasing the number of new researchers who can bring quantitative research skills and career readiness to their respective fields

Unusual phosphorus concentrations in the Florida Red Tide sea water

Beginning in late November 1946, and continuing at sporadic intervals until July 1947, a flowering of Gymnodinium sp. caused discolored water off the west coast of Florida. Millions of fish were killed along the coastline and this mortality was always directly associated with the presence of Gymnodinium (Gunter, Smith and Williams, 1947). The phenomenon has been popularly called the Red Tide

Creating Activating Events for Transformative Learning in a Prison Classroom

In this article, we interpreted, in light of Mezirow’s theory of transformative learning, interviews with 13 educators regarding their work with marginalized adult learners in prisons in the northeastern United States. Transformative learning may have been aided by the educators’ response to unplanned activating events, humor, and respect, and assumed roles of role model, and counselor. Teachers presented activating events they perceived supported resistant learners to alter their meaning schemes and revise their frames of reference. Administrators of educational programs for marginalized adult learners can help prison educators develop these pedagogical practices. Practitioners and researchers may wish to explore humor in restricted learning environments as well as the challenge mandatory prison classrooms present for the field of formal adult learning and transformative learning theory

Results of a study of the stability of cointegrating relations comprised of broad monetary aggregates

There is strong evidence of a stable “money demand” relationship for MZM and M2 through the 1990s. Though the M2 relationship breaks down somewhere around 1990, evidence has been accumulating that the disturbance is well characterized as a permanent upward shift in M2 velocity that began around 1990 and was largely over by 1994. This paper’s results support the hypothesis that households permanently reallocated a portion of their wealth from time deposits to mutual funds. This reallocation may have been induced by depository restructuring, but it could also be explained by appropriately measured opportunity cost.Demand for money

Exosome-mediated Transfer of αvβ3 Integrin from Tumorigenic to Nontumorigenic Cells Promotes a Migratory Phenotype.

The αvβ3 integrin is known to be highly upregulated during cancer progression and promotes a migratory and metastatic phenotype in many types of tumors. We hypothesized that the αvβ3 integrin is transferred through exosomes and, upon transfer, has the ability to support functional aberrations in recipient cells. Here, for the first time, it is demonstrated that αvβ3 is present in exosomes released from metastatic PC3 and CWR22Pc prostate cancer cells. Exosomal β3 is transferred as a protein from donor to nontumorigenic and tumorigenic cells as β3 protein or mRNA levels remain unaffected upon transcription or translation inhibition in recipient cells. Furthermore, it is shown that upon exosome uptake, de novo expression of an αvβ3 increases adhesion and migration of recipient cells on an αvβ3 ligand, vitronectin. To evaluate the relevance of these findings, exosomes were purified from the blood of TRAMP mice carrying tumors where the expression of αvβ3 is found higher than in exosomes from wild-type mice. In addition, it is demonstrated that αvβ3 is coexpressed with synaptophysin, a biomarker for aggressive neuroendocrine prostate cancer. IMPLICATIONS: Overall this study reveals that the αvβ3 integrin is transferred from tumorigenic to nontumorigenic cells via exosomes, and its de novo expression in recipient cells promotes cell migration on its ligand. The increased expression of αvβ3 in exosomes from mice bearing tumors points to its clinical relevance and potential use as a biomarker. Mol Cancer Res; 14(11); 1136-46. ©2016 AACR

A unique role for clathrin light chain A in cell spreading and migration.

Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show that following detachment and replating, CLCa is uniquely responsible for promoting efficient cell spreading and migration. Selective depletion of CLCa, but not of CLCb, reduced the initial phase of isotropic spreading of HeLa, H1299 and HEK293 cells by 60-80% compared to siRNA controls, and wound closure and motility by ∼50%. Surface levels of β1-integrins were unaffected by CLCa depletion. However, CLCa was required for effective targeting of FAK (also known as PTK2) and paxillin to the adherent surface of spreading cells, for integrin-mediated activation of Src, FAK and paxillin, and for maturation of focal adhesions, but not their microtubule-based turnover. Depletion of CLCa also blocked the interaction of clathrin with the nucleation-promoting factor WAVE complex, and altered actin distribution. Furthermore, preferential recruitment of CLCa to budding protrusions was also observed. These results comprise the first identification of CLCa-specific functions, with implications for normal and neoplastic integrin-based signaling and cell migration