298 research outputs found

    Poplar protease inhibitor expression differs in an herbivore specific manner

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    Evolution of a novel and adaptive floral scent in wild tobacco

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    Many plants emit diverse floral scents that mediate plant-environment interactions and attain reproductive success. However, how plants evolve novel adaptive floral volatiles remains unclear. Here, we show that in the wild tobacco, Nicotiana attenuata, a dominant species-specific floral volatile (benzyl acetone, BA) that attracts pollinators and deters florivore is synthesized by phenylalanine ammonia-lyase 4 (NaPAL4), isoflavone reductase 3 (NaIFR3), and chalcone synthase 3 (NaCHAL3). Transient expression of NaFIR3 alone in N. attenuata leaves is sufficient and necessary for ectopic foliar BA emissions, and the BA emission level is increased by co-expressing NaIFR3 with NaPAL4 and NaCHAL3. Independent changes in transcription in all three genes contributed to intraspecific variations of floral BA emission. However, among species, the gain-of-expression in NaIFR3 resulted in the biosynthesis of BA that was only found in N. attenuata. This study suggests that novel metabolic pathways associated with adaptation can arise via re-configurations of gene expression

    First results from post-COVID inpatient rehabilitation

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    BackgroundCOVID-19 is associated with various symptoms and psychological involvement in the long term. In view of the multifactorial triggering and maintenance of the post-COVID syndrome, a multimodal therapy with somatomedical and psychotherapeutic content is expedient. This paper compares the psychological stress of post-COVID patients and their course in rehabilitation to psychosomatic and psychocardiological patients.MethodObservational study with control-groups and clinical, standardized examination: psychological testing (BDI-II, HELATH-49), 6-MWT as somatic parameter, two measurement points (admission, discharge). Sample characteristics, including work related parameters, the general symptom-load and the course of symptoms during rehabilitation are evaluated.ResultsAt admission in all measures post-COVID patients were significantly affected, but less pronounced than psychosomatic or psychocardiological patients (BDI-II post-COVID = 19.29 ± 9.03, BDI-II psychosomatic = 28.93 ± 12.66, BDI-II psychocardiology = 24.47 ± 10.02). During rehabilitation, in all complaint domains and sub-groups, symptom severity was significantly reduced (effect sizes ranging from d = .34 to d = 1.22). Medium positive effects were seen on self-efficacy (d = .69) and large effects on activity and participation (d = 1.06) in post-COVID patients. In the 6-MWT, the walking distance improved by an average of 76.43 ± 63.58 meters (d = 1.22). Not a single patient deteriorated in walking distance, which would have been a possible sign of post exercise malaise (PEM).ConclusionPost-COVID patients have a slighter psychological burden as psychocardiological or psychosomatic patients. Although rehabilitation is not curative, post-COVID patients benefit significantly from the interventions and there were no signs of PEM

    Laser-In-Situ Monitoring of Combustion Processes

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    Several examples of laser in situ monitoring of combustion processes are presented. Using a frequency modulated 13CO2 waveguide laser, in situ concentrations of NH3 down to 1 ppm were measured at temperatures up to 600°C in waste incinerators and power or chemical plants. Following ignition of CH3OH-O2 mixtures by a TEA CO2 laser, gas temperature profiles were measured using rapid scanning tunable diode laser spectroscopy of CO molecules. In laminar CH4-air counterflow diffusion flames at atmospheric pressure absolute concentrations, temperatures, and collisional lifetimes of OH radicals were determined by 2-D and picosecond LIF and absorption spectroscopy. Two-dimensional LIF and Mie scattering were used to observe fuel injection and combustion in a diesel engine.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/86767/1/Sick52.pd
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