Real-time pair-feeding of animals

Automatic pair-feeding system was developed which immediately dispenses same amount of food to control animal as has been consumed by experimental animal that has free access to food. System consists of: master feeding system; slave feeding station; and control mechanism. Technique performs real time pair-feeding without attendant time lag

Automatic real-time pair-feeding system for animals

A pair feeding method and apparatus are provided for experimental animals wherein the amount of food consumed is immediately delivered to a normal or control animal so that there is a qualitative, quantitative and chronological correctness in the pair feeding of the two animals. This feeding mechanism delivers precisely measured amounts of food to a feeder. Circuitry is provided between master and slave feeders so that there is virtually no chance of a malfunction of the feeding apparatus, causing erratic results. Recording equipment is also provided so that an hourly record is kept of food delivery

Sensitivity of Greenland ice sheet projections to spatial resolution in higher-order simulations: the Alfred Wegener Institute (AWI) contribution to ISMIP6 Greenland using the Ice-sheet and Sea-level System Model (ISSM)

Projections of the contribution of the Greenland ice sheet to future sea-level rise include uncertainties primarily due to the imposed climate forcing and the initial state of the ice sheet model. Several state-of-the-art ice flow models are currently being employed on various grid resolutions to estimate future mass changes in the framework of the Ice Sheet Model Intercomparison Project for CMIP6 (ISMIP6). Here we investigate the sensitivity to grid resolution of centennial sea-level contributions from the Greenland ice sheet and study the mechanism at play. We employ the finite-element higher-order Ice-sheet and Sea-level System Model (ISSM) and conduct experiments with four different horizontal resolutions, namely 4, 2, 1 and 0.75 km. We run the simulation based on the ISMIP6 core climate forcing from the MIROC5 global circulation model (GCM) under the high-emission Representative Concentration Pathway (RCP) 8.5 scenario and consider both atmospheric and oceanic forcing in full and separate scenarios. Under the full scenarios, finer simulations unveil up to approximately 5 % more sea-level rise compared to the coarser resolution. The sensitivity depends on the magnitude of outlet glacier retreat, which is implemented as a series of retreat masks following the ISMIP6 protocol. Without imposed retreat under atmosphere-only forcing, the resolution dependency exhibits an opposite behaviour with approximately 5 % more sea-level contribution in the coarser resolution. The sea-level contribution indicates a converging behaviour below a 1 km horizontal resolution. A driving mechanism for differences is the ability to resolve the bedrock topography, which highly controls ice discharge to the ocean. Additionally, thinning and acceleration emerge to propagate further inland in high resolution for many glaciers. A major response mechanism is sliding, with an enhanced feedback on the effective normal pressure at higher resolution leading to a larger increase in sliding speeds under scenarios with outlet glacier retreat

ZmbZIP60 mRNA is spliced in maize in response to ER stress

<p>Abstract</p> <p>Background</p> <p>Adverse environmental conditions produce ER stress and elicit the unfolded protein response (UPR) in plants. Plants are reported to have two "arms" of the ER stress signaling pathway-one arm involving membrane-bound transcription factors and the other involving a membrane-associated RNA splicing factor, IRE1. IRE1 in yeast to mammals recognizes a conserved twin loop structure in the target RNA.</p> <p>Results</p> <p>A segment of the mRNA encoding ZmbZIP60 in maize can be folded into a twin loop structure, and in response to ER stress this mRNA is spliced, excising a 20b intron. Splicing converts the predicted protein from a membrane-associated transcription factor to one that is targeted to the nucleus. Splicing of ZmbZIP60 can be elicited in maize seedlings by ER stress agents such as dithiothreitol (DTT) or tunicamycin (TM) or by heat treatment. Younger, rather than older seedlings display a more robust splicing response as do younger parts of leaf, along a developmental gradient in a leaf. The molecular signature of an ER stress response in plants includes the upregulation of Binding Protein (BIP) genes. Maize has numerous BIP-like genes, and ER stress was found to upregulate one of these, ZmBIPb.</p> <p>Conclusions</p> <p>The splicing of ZmbZIP60 mRNA is an indicator of ER stress in maize seedlings resulting from adverse environmental conditions such as heat stress. ZmbZIP60 mRNA splicing in maize leads predictively to the formation of active bZIP transcription factor targeted to the nucleus to upregulate stress response genes. Among the genes upregulated by ER stress in maize is one of 22 BIP-like genes, ZmBIPb.</p