Study on Static Electrification of Palm Fatty Acid Ester (PFAE) Oil Using Mini Static Tester (Archive)

Palm fatty acid ester (PFAE) oil is an alternative insulating liquid of power transformer that has good biodegradable properties. As a part of insulating system of power transformer, PFAE should also has a low risk in term of static electrification that may degrade electrical strength of solid insulation due to charge accumulation at its surface during oil circulating. Charge potential value could describe the possibility of insulating oil in involving on charge accumulation at the surface of solid insulation. This can be calculated from charge density through electrostatic charge tendency (ECT) and volume resistivity. This study using mini-static-tester to measure ECT since it is easy to reproduce the specimen and suitable for standardization. Since PFAE has higher polarity than mineral oil, PFAE has larger charges than mineral oil. Even though static charges are estimated to generate on the pressboard surface easily, generated charges are estimated to escape from pressboard surface because of its lower resistivity. Variation of charge density and volume resistivity of several types (i.e. PFAE and mineral) and conditions (i.e. with-additives, non-additives, unused, and aged) of oil will influence to its charge potentia

Conjugated docosahexaenoic acid suppresses KPL-1 human breast cancer cell growth in vitro and in vivo: potential mechanisms of action

Introduction The present study was conducted to examine the effect of conjugated docosahexaenoic acid (CDHA) on cell growth, cell cycle progression, mode of cell death, and expression of cell cycle regulatory and/or apoptosis-related proteins in KPL-1 human breast cancer cell line. This effect of CDHA was compared with that of docosahexaenoic acid (DHA). Methods KPL-1 cell growth was assessed by colorimetric 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; cell cycle progression and mode of cell death were examined by flow cytometry; and levels of expression of p53, p21Cip1/Waf1, cyclin D1, Bax, and Bcl-2 proteins were examined by Western blotting analysis. In vivo tumor growth was examined by injecting KPL-1 cells subcutaneously into the area of the right thoracic mammary fat pad of female athymic mice fed a CDHA diet. Results CDHA inhibited KPL-1 cells more effectively than did DHA (50% inhibitory concentration for 72 hours: 97 μmol/l and 270 μmol/l, respectively). With both CDHA and DHA growth inhibition was due to apoptosis, as indicated by the appearance of a sub-G1 fraction. The apoptosis cascade involved downregulation of Bcl-2 protein; Bax expression was unchanged. Cell cycle progression was due to G0/G1 arrest, which involved increased expression of p53 and p21Cip1/Waf1, and decreased expression of cyclin D1. CDHA modulated cell cycle regulatory proteins and apoptosis-related proteins in a manner similar to that of parent DHA. In the athymic mouse system 1.0% dietary CDHA, but not 0.2%, significantly suppressed growth of KPL-1 tumor cells; CDHA tended to decrease regional lymph node metastasis in a dose dependent manner. Conclusion CDHA inhibited growth of KPL-1 human breast cancer cells in vitro more effectively than did DHA. The mechanisms of action involved modulation of apoptosis cascade and cell cycle progression. Dietary CDHA at 1.0% suppressed KPL-1 cell growth in the athymic mouse system.</p

Why NERICA is a successful innovation for African farmers

This paper responds to ‘Funding international agricultural research and the need to be noticed: a case study of NERICA rice’ by Stuart Orr, James Sumberg, Olaf Erenstein and Andreas Oswald, published in this issue of Outlook on Agriculture. In summary, the article by Orr et al, based on an internal WARDA document written in November 2003 and augmented with results from Internet searches, is outdated and does not seem to be fair, objective or useful. We invite the authors to visit WARDA or any of its partners in Sub-Saharan Africa for evidence of the impact of NERICA varieties or the other improved varieties and technologies that have been developed and disseminated by WARDA in recent years

A Cross-Species Analysis of a Mouse Model of Breast Cancer-Specific Osteolysis and Human Bone Metastases Using Gene Expression Profiling

<p>Abstract</p> <p>Background</p> <p>Breast cancer is the second leading cause of cancer-related death in women in the United States. During the advanced stages of disease, many breast cancer patients suffer from bone metastasis. These metastases are predominantly osteolytic and develop when tumor cells interact with bone. <it>In vivo </it>models that mimic the breast cancer-specific osteolytic bone microenvironment are limited. Previously, we developed a mouse model of tumor-bone interaction in which three mouse breast cancer cell lines were implanted onto the calvaria. Analysis of tumors from this model revealed that they exhibited strong bone resorption, induction of osteoclasts and intracranial penetration at the tumor bone (TB)-interface.</p> <p>Methods</p> <p>In this study, we identified and used a TB microenvironment-specific gene expression signature from this model to extend our understanding of the metastatic bone microenvironment in human disease and to predict potential therapeutic targets.</p> <p>Results</p> <p>We identified a TB signature consisting of 934 genes that were commonly (among our 3 cell lines) and specifically (as compared to tumor-alone area within the bone microenvironment) up- and down-regulated >2-fold at the TB interface in our mouse osteolytic model. By comparing the TB signature with gene expression profiles from human breast metastases and an <it>in vitro </it>osteoclast model, we demonstrate that our model mimics both the human breast cancer bone microenvironment and osteoclastogenesis. Furthermore, we observed enrichment in various signaling pathways specific to the TB interface; that is, TGF-β and myeloid self-renewal pathways were activated and the Wnt pathway was inactivated. Lastly, we used the TB-signature to predict cyclopenthiazide as a potential inhibitor of the TB interface.</p> <p>Conclusion</p> <p>Our mouse breast cancer model morphologically and genetically resembles the osteoclastic bone microenvironment observed in human disease. Characterization of the gene expression signature specific to the TB interface in our model revealed signaling mechanisms operative in human breast cancer metastases and predicted a therapeutic inhibitor of cancer-mediated osteolysis.</p

Clinical and immunological evaluation of anti-apoptosis protein, survivin-derived peptide vaccine in phase I clinical study for patients with advanced or recurrent breast cancer

<p>Abstract</p> <p>Background</p> <p>We previously reported that survivin-2B, a splicing variant of survivin, was expressed in various types of tumors and that survivin-2B peptide might serve as a potent immunogenic cancer vaccine. The objective of this study was to examine the toxicity of and to <b>c</b>linically and immunologically evaluate survivin-2B peptide in a phase I clinical study for patients with advanced or recurrent breast cancer.</p> <p>Methods</p> <p>We set up two protocols. In the first protocol, 10 patients were vaccinated with escalating doses (0.1–1.0 mg) of survivin-2B peptide alone 4 times every 2 weeks. In the second protocol, 4 patients were vaccinated with the peptide at a dose of 1.0 mg mixed with IFA 4 times every 2 weeks.</p> <p>Results</p> <p>In the first protocol, no adverse events were observed during or after vaccination. In the second protocol, two patients had induration at the injection site. One patient had general malaise (grade 1), and another had general malaise (grade 1) and fever (grade 1). Peptide vaccination was well tolerated in all patients. In the first protocol, tumor marker levels increased in 8 patients, slightly decreased in 1 patient and were within the normal range during this clinical trial in 1 patient. With regard to tumor size, two patients were considered to have stable disease (SD). Immunologically, in 3 of the 10 patients (30%), an increase of the peptide-specific CTL frequency was detected. In the second protocol, an increase of the peptide-specific CTL frequency was detected in all 4 patients (100%), although there were no significant beneficial clinical responses. ELISPOT assay showed peptide-specific IFN-γ responses in 2 patients in whom the peptide-specific CTL frequency in tetramer staining also was increased in both protocols.</p> <p>Conclusion</p> <p>This phase I clinical study revealed that survivin-2B peptide vaccination was well tolerated. The vaccination with survivin-2B peptide mixed with IFA increased the frequency of peptide-specific CTL more effectively than vaccination with the peptide alone, although neither vaccination could induce efficient clinical responses. Considering the above, the addition of another effectual adjuvant such as a cytokine, heat shock protein, etc. to the vaccination with survivin-2B peptide mixed with IFA might induce improved immunological and clinical responses.</p

Activin signaling as an emerging target for therapeutic interventions

After the initial discovery of activins as important regulators of reproduction, novel and diverse roles have been unraveled for them. Activins are expressed in various tissues and have a broad range of activities including the regulation of gonadal function, hormonal homeostasis, growth and differentiation of musculoskeletal tissues, regulation of growth and metastasis of cancer cells, proliferation and differentiation of embryonic stem cells, and even higher brain functions. Activins signal through a combination of type I and II transmembrane serine/threonine kinase receptors. Activin receptors are shared by multiple transforming growth factor-β (TGF-β) ligands such as myostatin, growth and differentiation factor-11 and nodal. Thus, although the activity of each ligand is distinct, they are also redundant, both physiologically and pathologically in vivo. Activin receptors activated by ligands phosphorylate the receptor-regulated Smads for TGF-β, Smad2 and 3. The Smad proteins then undergo multimerization with the co-mediator Smad4, and translocate into the nucleus to regulate the transcription of target genes in cooperation with nuclear cofactors. Signaling through receptors and Smads is controlled by multiple mechanisms including phosphorylation and other posttranslational modifications such as sumoylation, which affect potein localization, stability and transcriptional activity. Non-Smad signaling also plays an important role in activin signaling. Extracellularly, follistatin and related proteins bind to activins and related TGF-β ligands, and control the signaling and availability of ligands

Rapid Method of Brabender Viscograph and its Application to Analysis of a Large Number of Samples

It is possible to establish a rapid method of Brabender viscography by reducing the reaction time for gelatinization. A rapid method was examined by increasing the heating and cooling speed of the suspension and evaluated by the application to a large number of samples for its practical use. (1) Increase of heating and cooling speed to 3°C/min in the rapid method reduced the measuring time of the existing method by half to 47 minutes and made possible to analyze 8 samples a day. (2) Reproducibility in standard deviation of the rapid method was 7.6 BU which was more accurate than the existing method. High significant correlation between the two methods was observed for each parameter. (3) Each method needs to be performed independently due to the large difference in the analytical data in several parameters between the two methods. (4) The correlations between the viscosity parameters are often different among the rice species. Therefore, every viscosity parameter in the cooled condition is required to be directly analyzed for its evaluation. From these data, it was indicated that the rapid method can be used for the evaluation of the viscosity characteristics of a large number of samples with adequate accuracy using existing Brabender viscographs

Agro-morphological characterization of a population of introgression lines derived from crosses between IR 64 (Oryza sativa indica) and TOG 5681 (Oryza glaberrima) for drought tolerance

The study evaluated effects of drought on some agro-morphological traits of 60 rice genotypes comprising 54 introgression lines with their parents, IR 64 (Oryza sativa) and TOG 5681 (Oryza glaberrima) and four NERICA-L varieties developed from the same parents for comparison. The genotypes were subjected either to full irrigation from sowing to maturity (control) or to 21-day drought applied by stopping irrigation from the 45th day after sowing (DAS) onward (drought) in the dry seasons of 2006 and 2007-2008. Plant height, spikelet fertility, grain yield and leaf area at harvesting were consistently reduced by drought in both seasons. Values of leaf temperature, leaf rolling, leaf tip drying, leaf blast, days from seeding to flowering and maturity were higher under drought. The results on SPAD and number of tillers were not consistent. Significant relationship (P < 0.05) was observed between all traits evaluated and grain yield under drought. Introgression lines, SEN-L13-2, MPL-15-3, SEN-L10-1, SEN-L26-3 and SEN-L21-2 showed significantly higher yield than the highest yield NERICA-L variety (all of them had higher yield than the parents). Among them, SEN-L13-2 showed the lowest yield loss by drought and MPL-15-3 had high yield potential and considerably low yield loss by drought