152 research outputs found
Cas9 gRNA engineering for genome editing, activation and repression
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.National Human Genome Research Institute (U.S.) (P50 HG005550)United States. Department of Energy (DE-FG02-02ER63445)Wyss Institute for Biologically Inspired EngineeringUnited States. Army Research Office (DARPA W911NF-11-2-0054)National Science Foundation (U.S.)United States. National Institutes of Health (5R01CA155320-04)United States. National Institutes of Health (P50 GM098792)National Cancer Institute (U.S.) (5T32CA009216-34)Massachusetts Institute of Technology. Department of Biological EngineeringHarvard Medical School. Department of GeneticsDefense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006
Genetic and neurological evaluation in a sample of individuals with pervasive developmental disorders
Neue Methode zur Unterscheidung von Frauenmilch und Kuhmilch, zugleich ein Beitrag zum Studium des lipolytischen Milchferments
Ăber die Bedeutung der Kuhmilchmolke fĂŒr die Entstehung akuter diarrhöischer ErnĂ€hrungsstörungen im SĂ€uglingsalter
Ăber die gegenwĂ€rtige Ausbreitung der Tuberkulose und der tuberkulösen Infektion unter den Berliner Kindern
Eine Indicatorenmethode zur AciditÀtsmessung im Magen- und Darmsaft beim Erwachsenen und beim SÀugling
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