762 research outputs found
Calcite distribution and orientation in the tergite exocuticle of the isopods porcellio scaber and armadillidium vulgare (Oniscidea, Crustacea) - A combined FE-SEM, polarized SCm-RSI and EBSD study
The crustacean cuticle is a bio-composite consisting of hierarchically organized chitin-protein fibres, reinforced with calcite, amorphous calcium carbonate and phosphates. Comparative studies revealed that the structure and composition of tergite cuticle of terrestrial isopods is adapted to the habitat of the animals, and to their behavioural patterns to avoid predation. In this contribution we use FE-SEM, polarized SCm-RSI and EBSD to investigate micro- and nano-patterns of mineral phase distribution and crystal orientation within the tergite cuticle of the two terrestrial isopod species Armadillidium vulgare and Porcellio scaber. The results show that the proximal regions of the exocuticle contain both calcite and ACC, with ACC located within the pore canals. Calcite forms hierarchically organised mesocrystalline aggregates of similar crystallographic orientation. Surprisingly, c-axis orientation preference is horizontal in regard to the local cuticle surface for both species, in contrast to mollusc and brachiopod shell structures in which the c-axis is always perpendicular to the shell surface. The overall sharpness of calcite crystal orientation is weak compared to that of mollusc shells. However, there are considerable differences in texture sharpness between the two isopod species. In the thick cuticle of the slow-walking A. vulgare calcite is more randomly oriented resulting in more isotropic mechanical properties of the cuticle. In contrast, the rather thin and more flexible cuticle of the fast- running P. scaber texture sharpness is stronger with a preference of c-axis orientation being parallel to the bilateral symmetry-plane of the animal, leading to more anisotropic mechanical properties of the cuticle. These differences may represent adaptations to different external and/or internal mechanical loads the cuticle has to resist during predatory attempts
Metacarpophalangeal joint loads during bonobo locomotion: model predictions vs. proxies
The analysis of internal trabecular and cortical bone has been an informative tool for drawing inferences about behaviour in extant and fossil primate taxa. Within the hand, metacarpal bone architecture has been shown to correlate well with primate locomotion; however, the extent of morphological differences across taxa is unexpectedly small given the variability in hand use. One explanation for this observation is that the activity-related differences in the joint loads acting on the bone are simply smaller than estimated based on commonly used proxies (i.e. external loading and joint posture), which neglect the influence of muscle forces. In this study, experimental data and a musculoskeletal finger model are used to test this hypothesis by comparing differences between climbing and knuckle-walking locomotion of captive bonobos (Pan paniscus) based on (i) joint load magnitude and direction predicted by the models and (ii) proxy estimations. The results showed that the activity-related differences in predicted joint loads are indeed much smaller than the proxies would suggest, with joint load magnitudes being almost identical between the two locomotor modes. Differences in joint load directions were smaller but still evident, indicating that joint load directions might be a more robust indicator of variation in hand use than joint load magnitudes. Overall, this study emphasizes the importance of including muscular forces in the interpretation of skeletal remains and promotes the use of musculoskeletal models for correct functional interpretations
Theoretical characterization of a model of aragonite crystal orientation in red abalone nacre
Nacre, commonly known as mother-of-pearl, is a remarkable biomineral that in
red abalone consists of layers of 400-nm thick aragonite crystalline tablets
confined by organic matrix sheets, with the crystal axes of the
aragonite tablets oriented to within 12 degrees from the normal to the
layer planes. Recent experiments demonstrate that this orientational order
develops over a distance of tens of layers from the prismatic boundary at which
nacre formation begins.
Our previous simulations of a model in which the order develops because of
differential tablet growth rates (oriented tablets growing faster than
misoriented ones) yield patterns of tablets that agree qualitatively and
quantitatively with the experimental measurements. This paper presents an
analytical treatment of this model, focusing on how the dynamical development
and eventual degree of order depend on model parameters. Dynamical equations
for the probability distributions governing tablet orientations are introduced
whose form can be determined from symmetry considerations and for which
substantial analytic progress can be made. Numerical simulations are performed
to relate the parameters used in the analytic theory to those in the
microscopic growth model. The analytic theory demonstrates that the dynamical
mechanism is able to achieve a much higher degree of order than naive estimates
would indicate.Comment: 20 pages, 3 figure
Foot Bone in Vivo: Its Center of Mass and Centroid of Shape
This paper studies foot bone geometrical shape and its mass distribution and
establishes an assessment method of bone strength. Using spiral CT scanning,
with an accuracy of sub-millimeter, we analyze the data of 384 pieces of foot
bones in vivo and investigate the relationship between the bone's external
shape and internal structure. This analysis is explored on the bases of the
bone's center of mass and its centroid of shape. We observe the phenomenon of
superposition of center of mass and centroid of shape fairly precisely,
indicating a possible appearance of biomechanical organism. We investigate two
aspects of the geometrical shape, (i) distance between compact bone's centroid
of shape and that of the bone and (ii) the mean radius of the same density bone
issue relative to the bone's centroid of shape. These quantities are used to
interpret the influence of different physical exercises imposed on bone
strength, thereby contributing to an alternate assessment technique to bone
strength.Comment: 9 pages, 4 figure
A pilot study of cardiac MRI in breast cancer survivors after cardiotoxic chemotherapy and three-dimensional conformal radiotherapy
Purpose/Objectives: Node-positive breast cancer patients often receive chemotherapy and regional nodal irradiation. The cardiotoxic effects of these treatments, however, may offset some of the survival benefit. Cardiac magnetic resonance (CMR) is an emerging modality to assess cardiac injury. This is a pilot trial assessing cardiac damage using CMR in patients who received anthracycline-based chemotherapy and three-dimensional conformal radiotherapy (3DCRT) regional nodal irradiation using heart constraints.
Materials and Methods: Node-positive breast cancer patients (2000-2008) treated with anthracycline-based chemotherapy and 3DCRT regional nodal irradiation (including the internal mammary chain nodes) with heart ventricular constraints (V25 \u3c 10%) were invited to participate. Cardiac tissues were contoured and analyzed separately for whole heart (pericardium) and for combined ventricles and left atrium (myocardium). CMR obtained ventricular function/dimensions, late gadolinium enhancement (LGE), global longitudinal strain (GLS), and extracellular volume fraction (ECV) as measures of cardiac injury and/or early fibrosis. CMR parameters were correlated with dose-volume constraints using Spearman correlations.
Results: Fifteen left-sided and five right-sided patients underwent CMR. Median diagnosis age was 50 (32-77). No patients had baseline cardiac disease before regional nodal irradiation. Median time after 3DCRT was 8.3 years (5.2-14.4). Median left-sided mean heart dose (MHD) was 4.8 Gy (1.1-11.2) and V25 was 5.7% (0-12%). Median left ventricular ejection fraction (LVEF) was 63%. No abnormal LGE was observed. No correlations were seen between whole heart doses and LVEF, LV mass, GLS, or LV dimensions. Increasing ECV did not correlate with increased heart or ventricular doses. However, correlations between higher LV mass and ventricular mean dose, V10, and V25 were seen.
Conclusion: At a median follow-up of 8.3 years, this cohort of node-positive breast cancer patients who received anthracycline-based chemotherapy and regional nodal irradiation had no clinically abnormal CMR findings. However, correlations between ventricular mean dose, V10, and V25 and LV mass were seen. Larger corroborating studies that include advanced techniques for measuring regional heart mechanics are warranted
The Influence of Mineralization on Intratrabecular Stress and Strain Distribution in Developing Trabecular Bone
The load-transfer pathway in trabecular bone is largely determined by its architecture. However, the influence of variations
in mineralization is not known. The goal of this study was to examine the influence of inhomogeneously distributed degrees
of mineralization (DMB) on intratrabecular stresses and strains. Cubic mandibular condylar bone specimens from fetal and newborn
pigs were used. Finite element models were constructed, in which the element tissue moduli were scaled to the local DMB. Disregarding
the observed distribution of mineralization was associated with an overestimation of average equivalent strain and underestimation
of von Mises equivalent stress. From the surface of trabecular elements towards their core the strain decreased irrespective
of tissue stiffness distribution. This indicates that the trabecular elements were bent during the compression experiment.
Inhomogeneously distributed tissue stiffness resulted in a low stress at the surface that increased towards the core. In contrast,
disregarding this tissue stiffness distribution resulted in high stress at the surface which decreased towards the core. It
was concluded that the increased DMB, together with concurring alterations in architecture, during development leads to a
structure which is able to resist increasing loads without an increase in average deformation, which may lead to damage
Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
Osteoporotic fragility fractures were hypothesized to be related to changes in bone material properties and not solely to reduction in bone mass. We studied cortical bone from the superior and inferior sectors of whole femoral neck sections from five female osteoporotic hip fracture cases (74â92Â years) and five nonfractured controls (75â88Â years). The typical calcium content (CaPeak) and the mineral particle thickness parameter (T) were mapped in large areas of the superior and inferior regions using quantitative backscattered electron imaging (qBEI) and scanning small-angle X-ray scattering, respectively. Additionally, indentation modulus (E) and hardness (H) (determined by nanoindentation) were compared at the local level to the mineral content (CaInd) at the indent positions (obtained from qBEI). CaPeak (â2.2%, PÂ =Â 0.002), CaInd (â1.8%, PÂ =Â 0.048), E (â5.6%, PÂ =Â 0.040), and H (â6.0%, PÂ =Â 0.016) were significantly lower for the superior compared to the inferior region. Interestingly, CaPeak as well as CaInd were also lower (â2.6%, PÂ =Â 0.006, and â3.7%, PÂ =Â 0.002, respectively) in fracture cases compared to controls, while E and H did not show any significant reduction. T values were in the normal range, independent of region (PÂ =Â 0.181) or fracture status (PÂ =Â 0.551). In conclusion, it appears that the observed femoral neck fragility is associated with a reduced mineral content, which was not accompanied by a reduction in stiffness and hardness of the bone material. This pilot study suggests that a stiffening process in the organic matrix component contributes to bone fragility independently of mineral content
COMPONENT-BASED EMBEDDED SOFTWARE ENGINEERING: DEVELOPMENT FRAMEWORK, QUALITY ASSURANCE AND A GENERIC ASSESSMENT ENVIRONMENT
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