678 research outputs found
Point-of-care diagnostics of covid-19: From current work to future perspectives
Coronaviruses have received global concern since 2003, when an outbreak caused by SARSâCoV emerged in China. Later on, in 2012, the MiddleâEast respiratory syndrome spread in Saudi Arabia, caused by MERSâCoV. Currently, the global crisis is caused by the pandemic SARSâ CoVâ2, which belongs to the same lineage of SARSâCoV. In response to the urgent need of diagnostic tools, several labâbased and biosensing techniques have been proposed so far. Five main areas have been individuated and discussed in terms of their strengths and weaknesses. The cellâculture detection and the microneutralization tests are still considered highly reliable methods. The genetic screening, featuring the wellâestablished Realâtime polymerase chain reaction (RTâPCR), represents the gold standard for virus detection in nasopharyngeal swabs. On the other side, immunoassays were developed, either by screening/antigen recognition of IgM/IgG or by detecting the whole virus, in blood and sera. Next, proteomic massâspectrometry (MS)âbased methodologies have also been proposed for the analysis of swab samples. Finally, virus-biosensing devices were efficiently designed. Both electrochemical immunosensors and eyeâbased technologies have been described, showing detection times lower than 10 min after swab introduction. Alternative to swabâbased techniques, lateral flow pointâofâcare immunoassays are already commercially available for the analysis of blood samples. Such biosensing devices hold the advantage of being portable for onâsite testing in hospitals, airports, and hotspots, virtually without any sample treatment or complicated lab precautions
Clinical Pathological Analysis of Surgically Resected Superficial Esophageal Carcinoma to Determine Criteria for Deciding on Treatment Strategy
We performed a clinical pathological study of conventionally resected superficial esophageal carcinomas since this type of lesion has been increasing, in order to develop criteria of determination for therapeutic strategies. Pathological studies were performed on specimens obtained by radical surgical resection in 133 cases of superficial esophageal cancer. Evaluation was performed in terms of the gross classification of the lesion type, depth of invasion, lymph node metastasis, vascular invasion, size of the lesion, outcome, etc. In 0-I, 0-IIc+0-IIa, and 0-III type submucosal cancer lesions the rate of metastasis to lymph nodes was more than 40%, but in 0-IIa and 0-IIb mucosal cancer cases no lymph node metastasis was observed. 0-IIc type lesions showed a wide range of invasiveness, ranging from m1 to sm3. In cases with m1 or m2 invasion, no lymph node or lymph-vessel invasion was recognized, but in m3, sm1, sm2, and sm3 cases lymph node metastasis was recognized in 12.5%, 22.2%, 44.0% and 47.4%, respectively. In 47% of lesions with a greatest dimension of less than 30 mm invasion was limited to the mucosa. Seventy-two percent of m1 and m2 cases were 30 mm in size or less. Lymph node metastasis was recognized in only 16.7% of cases less than 30 mm in size, but in cases of lesions 30 mm or more the rate of lymph node metastasis was 35.8%. 0-IIb and 0-IIa type lesions are indications for endoscopic esophageal mucosal resection (EEMR), while 0-I, 0-IIc+0-IIa, and 0-III lesions should be candidates for radical surgical resection. In the 0-IIc category, lesions in which the depression is relatively flat and with a finely granular surface are indications for EEMR, but those cases in which the surface of depression shows granules of varying sizes should be treated with radical surgical resection. Cases of 0-IIa type 30 mm or larger in greatest dimension which have a gently sloping protruding margin shoulder or reddening should be treated with caution, but EEMR can be performed first and subsequent therapeutic strategy decided on, based on the pathological findings of the specimen
Curriculum development for explorative proving in lower secondary school geometry: Focusing on the levels of planning and constructing a proof
This is the final published version. Also available from Frontiers via the DOI in this record.Mathematics education continues to emphasize explorative proving, wherein proving involves producing statements, producing proofs, looking back (examining, improving, and advancing) at these products, and the interactions among these aspects. This study aims to develop an intended explorative proving mathematics curriculum by focusing on students' ability to plan and construct proofs. We first set Levels 1 and 2 of âplanning a proofâ and âconstructing a proof,â respectively, and Level 0 as the starting point of the learning progression where there is no differentiation between planning and constructing. Next, we combined them, and set nine learning levels in addition to âlooking backâ as the characteristics of explorative proving. Then, we elucidated two learning progressions of explorative proving as a curriculum framework considering the relationship between planning and constructing a proof. To develop the curriculum based on these learning progressions, we made corresponding tables of units with these learning progressions according to the units of Japan's national Course of Study, and then showed an example of localizing one of the progressions and its effects by the implemented curriculum. By adopting the method of lesson study and a design experiment, we implemented geometry lessons for 8th graders that aim to shift the progression through the learning levels. The results clarify the advantages and limitations of the developed curriculum, which enabled us to refine a more robust curriculum. Finally, we identify the characteristics of this approach to curriculum development of explorative proving and the necessity of the method of lesson study and design experiment as a realistic dimension of curriculum development and improvemenGrant-in-Aid for Scientific Research, Ministry of Education, Culture, Sports, Science, and Technology, Japa
Topographic map reorganization in cat area 17 after early monocular retinal lesions
Neither discrete peripheral retinal lesions nor the normal optic disk produces obvious holes in one\u27s percept of the world because the visual brain appears to perceptually fill in these blind spots. Where in the visual brain or how this filling in occurs is not well understood. A prevailing hypothesis states that topographic map of visual cortex reorganizes after retinal lesions, which sews up the hole in the topographic map representing the deprived area of cortex (cortical scotoma) and may lead to perceptual filling in. Since the map reorganization does not typically occur unless retinotopically matched lesions are made in both eyes, we investigated the conditions in which monocular retinal lesions can induce comparable map reorganization. We found that following monocular retinal lesions, deprived neurons in cat area 17 can acquire new receptive fields if the lesion occurred relatively early in life (8 weeks of age) and the lesioned cats experienced a substantial period of recovery (\u3e3 years). Quantitative determination of the monocular and binocular response properties of reactivated units indicated that responses to the lesioned eye for such neurons were remarkably robust, and that the receptive-field properties for the two eyes were generally similar. Moreover, excitatory or inhibitory binocular interactions were found in the majority of experimental units when the two eyes were activated together. These results are consistent with the hypothesis that map reorganization after monocular retinal lesions require experience-dependent plasticity and may be involved in the perceptual filling in of blind spots due to retinal lesions early in life
High-performance electric double-layer capacitors using mass-produced multi-walled carbon nanotubes
The original publication is available at www.springerlink.com.https://doi.org/10.1007/s00339-005-3398-7 | https://doi.org/10.1007/s00339-005-3398-7ArticleApplied Physics A. 82(4):559-565 (2006)journal articl
Preparation and characterization of bamboo-based activated carbons as electrode materials for electric double layer capacitors
ArticleCarbon. 44(8):1592-1595 (2006)journal articl
New apparatus for DTA at 2000 bar: thermodynamic studies on Au, Ag, Al and HTSC oxides
A new DTA (Differential Thermal Analysis) device was designed and installed
in a Hot Isostatic Pressure (HIP) furnace in order to perform high-pressure
thermodynamic investigations up to 2 kbar and 1200C. Thermal analysis can be
carried out in inert or oxidising atmosphere up to p(O2) = 400 bar. The
calibration of the DTA apparatus under pressure was successfully performed
using the melting temperature (Tm) of pure metals (Au, Ag and Al) as standard
calibration references. The thermal properties of these metals have been
studied under pressure. The values of DV (volume variation between liquid and
solid at Tm), ROsm (density of the solid at Tm) and ALPHAm (linear thermal
expansion coefficient at Tm) have been extracted. A very good agreement was
found with the existing literature and new data were added. This HP-DTA
apparatus is very useful for studying the thermodynamics of those systems where
one or more volatile elements are present, such as high TC superconducting
oxides. DTA measurements have been performed on Bi,Pb(2223) tapes up to 2 kbar
under reduced oxygen partial pressure (p(O2) = 0.07 bar). The reaction leading
to the formation of the 2223 phase was found to occur at higher temperatures
when applying pressure: the reaction DTA peak shifted by 49C at 2 kbar compared
to the reaction at 1 bar. This temperature shift is due to the higher stability
of the Pb-rich precursor phases under pressure, as the high isostatic pressure
prevents Pb from evaporating.Comment: 6 figures, 3 tables, Thermodynamics, Thermal property, Bi-2223,
fundamental valu
Isolation of an Arabidopsis thaliana Mutant, mto1, That Overaccumulates Soluble Methionine (Temporal and Spatial Patterns of Soluble Methionine Accumulation)
- âŠ