Machine Learning to Predict Risk of Relapse Using Cytologic Image Markers in Patients With Acute Myeloid Leukemia Posthematopoietic Cell Transplantation.

Allogenic hematopoietic stem-cell transplant (HCT) is a curative therapy for acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Relapse post-HCT is the most common cause of treatment failure and is associated with a poor prognosis. Pathologist-based visual assessment of aspirate images and the manual myeloblast counting have shown to be predictive of relapse post-HCT. However, this approach is time-intensive and subjective. The premise of this study was to explore whether computer-extracted morphology and texture features from myeloblasts' chromatin patterns could help predict relapse and prognosticate relapse-free survival (RFS) after HCT. In this study, Wright-Giemsa-stained post-HCT aspirate images were collected from 92 patients with AML/MDS who were randomly assigned into a training set (S <sub>t</sub> = 52) and a validation set (S <sub>v</sub> = 40). First, a deep learning-based model was developed to segment myeloblasts. A total of 214 texture and shape descriptors were then extracted from the segmented myeloblasts on aspirate slide images. A risk score on the basis of texture features of myeloblast chromatin patterns was generated by using the least absolute shrinkage and selection operator with a Cox regression model. The risk score was associated with RFS in S <sub>t</sub> (hazard ratio = 2.38; 95% CI, 1.4 to 3.95; P = .0008) and S <sub>v</sub> (hazard ratio = 1.57; 95% CI, 1.01 to 2.45; P = .044). We also demonstrate that this resulting signature was predictive of AML relapse with an area under the receiver operating characteristic curve of 0.71 within S <sub>v</sub> . All the relevant code is available at GitHub. The texture features extracted from chromatin patterns of myeloblasts can predict post-HCT relapse and prognosticate RFS of patients with AML/MDS

Utilization of a Novel Chitosan/Clay/Biochar Nanobiocomposite for Immobilization of Heavy Metals in Acid Soil Environment

An organic–inorganic composite of chitosan, nanoclay, and biochar (named as MTCB) was chosen to develop a bionanocomposite to simultaneously immobilize Cu, Pb, and Zn metal ions within the contaminated soil and water environments. The composite material was structurally and chemically characterized with the XRD, TEM, SEM, BET, and FT-IR techniques. XRD and TEM results revealed that a mixed exfoliated/intercalated morphology was formed upon addition of small amounts of nanoclay (5% by weight). Batch adsorption experiments showed that the adsorption capacity of MTCB for Cu2+, Pb2+, and Zn2+ were much higher than that of the pristine biochar sample (121.5, 336, and 134.6 mg g−1 for Cu2+, Pb2+, and Zn2+, respectively). The adsorption isotherm for Cu2+ and Zn2+ fitted satisfactorily to a Freundlich model while the isotherm of Pb2+ was best represented by a Temkin model. That the adsorption capacity increased with increasing temperature is indicative of the endothermic nature of the adsorption process. According to the FTIR analysis, the main mechanism involved in immobilization of metals is binding with –NH2 groups. Results from this study indicated that modification of biochar by chitosan/clay nanocomposite enhances its potential capacity for immobilization of heavy metals, rendering the bionanocomposite into an efficient heavy metal sorbent in mine-impacted acidic waters and soils