Penggunaan Pelacak Nonradioaktif (Digoxigenin-dna Probe) Untuk Mendeteksi Peanut Stripe Virus

The use of nonradioactive probe (Digoxigenin-DNA) for detection of peanut stripe virus. The objective of this experiment was to develop the nonradioactive-labeled probe to detect peanut stripe virus (PStV) in peanut leaves and seeds. Digoxigenin labeled cDNA (dig-DNA probe) was synthesized from recombinant plasmid (pHS1.23) using polymerase chain reaction (PCR). The probe containing 1.195 bp (base pair) corresponding to 3' termini, included part of NIb (nuclear inclusion body) gene, coat protein gene, and 3' untranslated region of PStV genome was used to detect the existence of PStV in peanut leaves and seeds of infected peanut plants

Respon Beberapa Genotipe Kedelai Terhadap Infeksi Cpmmv (Cowpea Mild Mottle Virus)

Response of various soybean genotipes against cowpea mild mottle virus (CPMMV) infection. The green house experiment was conducted to investigate the effect of CPMMV infection on growth and yield of various soybean varieties. Treatments were arranged in completely randomized block design in split plot experiment. Mainplots were virus-inoculated and uninoculated plants; subplots were eight soybean varieties: Wilis, Orba, MLG 2521, Taichung, B 3570, Dieng, Tidar, and Pangrango. The results of this experiment showed that CPMMV infection decreased vegetative growth and yield of soybean plant. The decrease of dry weigth of soybean plant proved that CPMMV infection inhibited the vegetative growth. Taichung variety susceptible to CPMMV was indicated by significant reduction of the growth and yield. B3570 line tolerance to CPMMV infection was proved by lower reduction of the yield

Proximity (Mis)perception: Public Awareness of Nuclear, Refinery, and Fracking Sites

This is the final version. Available on open access from Wiley via the DOI in this recordWhether on grounds of perceived safety, aesthetics, or overall quality of life, residents may wish to be aware of nearby energy sites such as nuclear reactors, refineries, and fracking wells. Yet people are not always accurate in their impressions of proximity. Indeed, our data show that only 54% of Americans living within 25 miles of a nuclear site say they do, and even fewer fracking-proximal (30%) and refinery-proximal (24%) residents respond accurately. In this article, we analyze factors that could either help people form more accurate perceptions or distort their impressions of proximity. We evaluate these hypotheses using a large national survey sample and corresponding geographic information system (GIS) data. Results show that among those living in close proximity to energy sites, those who perceive greater risk are less likely to report living nearby. Conversely, social contact with employees of these industries increases perceived proximity regardless of actual distance. These relationships are consistent across each site type we examine. Other potential factors—such as local news use—may play a role in proximity perception on a case-by-case basis. Our findings are an important step toward a more generalizable understanding of how the public forms perceptions of proximity to risk sites, showing multiple potential mechanisms of bias.European Union Horizon 202

Oscillation Criteria for a Certain Class of Second Order Emden-Fowler Dynamic Equations

By means of Riccati transformation techniques we establish some oscillation criteria for the second order Emden-Fowler dynamic equation on a time scale. Such equations contain the classical Emden-Fowler equation as well as their discrete counterparts. The classical oscillation results of Atkinson (in the superlinear case) and Belohorec (in the sublinear case) are extended in this paper to Emden-Fowler dynamic equations on any time scale

Coopetition as a Small Business Strategy: Implications for Performance

This paper explores coopetition, a strategy that combines cooperation and competition in addressing relationships between firms. We examine the underlying nature of coopetition, and evaluate the extent to which it represents a relevant strategy for small firms. Inherent problems are identified when attempting to collaborate with competitors. We propose an approach to measuring the coopetitive tendencies of small firms. The measurement approach centers on three underlying dimensions: mutual benefit, trust, and commitment. Applying this approach, we assess the relationship between coopetition and firm performance. Based on a survey of 647 small firms in Turkey, a strong, positive relationship is identified. Theoretical and managerial implications are drawn from the findings

Effects of Saccharomyces boulardii on antibiotic induced orocecal transit in rats

Clarithromycin is an antibiotic widely used for Helicobacter pylori (H. pylori) eradication and together with amoxicillin and proton pump inhibitors they constitute the first line triple treatment regimen against H. pylori. Diarrhoea is one of the major drawbacks during H. pylori eradication and is majorly attributed to clarithromycin, while Saccharomyces boulardii is a probiotic and is shown to be effective in the treatment of antibiotic associated diarrhoea. We aimed to evaluate the effect of clarithromycin on orocecal transit in rats and to identify whether the supplementation with S. boulardii has a role on orocecal transit index. Adult rats of both sexes were divided into two groups to determine immediate or chronic effects of S. boulardii and clarithromycin on orocecal transit. The first group was given single dose of the test drug, while the second group received the test drugs for one week through orogastric intubation. Both groups were randomly distributed into four subgroups; the placebo group (group A), the S. boulardii group (group B), the clarithromycin group (group C), and the co-administration that is clarithromycin plus S. boulardii group (group D). Rats were given 20 mg kg−1 clarithromycin and 500 mg kg−1S. boulardii. We did not find any difference among the subgroups in group 1, where only single dose of the test drugs was administered. In chronic administration group, that is group 2, significant differences among the subgroups were observed (P=0.004). Post-hoc comparisons of orocecal transit index between group “2A and 2C” and “2C and 2D” were significantly different (P=0.013 and P=0.005, respectively). Our results show that long term clarithromycin administration leads to rapid orocecal transit index and S. boulardii supplementation to clarithromycin can abolish this adverse effect in rats. Those findings suggest the beneficial use of S. boulardii in H. pylori eradication regimens