Palaeoenvironmental signatures revealed from rare earth element (REE) compositions of vertebrate microremains of the Vesiku Bone Bed (Homerian, Wenlock), Saaremaa Island, Estonia

The Estonian Journal of Earth Sciences is an open access journal and applies the Creative Commons Attribution 4.0 International License CC BY to all its papers (http://creativecommons.org/licenses/by/4.0/). The attached file is the published version of the article

Creating novel approaches to mitigate aflatoxin risk in food and feed with lactic acid bacteria - mold growth inhibition and aflatoxin binding

Aflatoxins, produced by Aspergillus fungi, are ubiquitous toxins and they can present a severe health risk to humans and animals if contaminated food and feed is consumed. Fungi live in the soil and on the surface of the crop and Aspergillus species are dominant in favorable conditions of maize cultivation areas. Climate change could threaten the production of safe food by promoting Aspergillus growth and aflatoxin production in food and feed. A novel biological approach using lactic acid bacteria (LAB) could reduce the health risks of aflatoxins through inhibiting mold growth, thus aflatoxin production and by binding existing aflatoxins. LAB are commonly used in fermented food production; they are also known to inhibit mold growth and interact with aflatoxins. LAB provide a potential novel approach to mitigate the mould growth and aflatoxin production in maize during storage and after food consumption. Mold growth inhibition by certain LAB strains may be caused by competition for resources between bacterial cells and fungi and/or production of antifungal compounds such as organic acids. Aflatoxin binding is more complex. Binding is a reversible reaction, which occurs on bacterial surfaces and involves interaction with carbohydrates, peptidoglycan and to some extent protein structures. Aflatoxin binding seems to be highly related to strain, matrix, temperature, pH, incubation time and related conditions. There are two different aspects of aflatoxin risk mitigation in this research. First is the fungal growth inhibition with LAB and second is aflatoxin binding from food and feed with LAB. We have isolated 200 strains of bacteria from 21 different indigenous fermented dairy and cereal products prepared locally in different parts of Kenya. Firstly, these strains are being tested for their growth inhibition abilities against aflatoxin producing Aspergillus fungi in laboratory conditions. Secondly, the same strains are tested for their abilities to bind and retain aflatoxin M1 and B1. Later, these same effective strains will be tested in various food and feed matrices against Aspergillus growth and then the ones with most potential will be identified. This approach aims at providing a safe method of reducing aflatoxin absorption in human gastrointestinal tract after ingesting fermented maize or dairy products, which are contaminated with aflatoxins. Novel biological methods can have a role in preventing toxic effects of aflatoxins in food and feed. Exploitation of LAB is a good option for existing methods as LAB are generally recognized as safe. This research is done as part of FoodAfrica programme, which is a research, and development programme and the main funding agency being Finnish Ministry for Foreign Affairs. The research is partnering with MTT Agrifood Research Finland and ILRI International Livestock Research Institute

Safety and tolerability of NXY-059 for acute intracerebral hemorrhage: the CHANT trial

<p><b>Background and Purpose:</b> NXY-059 is a free radical-trapping neuroprotectant developed for use in acute ischemic stroke. To facilitate prompt administration of treatment, potentially before neuroimaging, we investigated the safety of NXY-059 in patients with intracerebral hemorrhage (ICH).</p> <p><b>Methods:</b> We randomized 607 patients within 6 hours of acute ICH to receive 2270 mg intravenous NXY-059 over 1 hour and then up to 960 mg/h over 71 hours, or matching placebo, in addition to standard care. The primary outcome was safety: the mortality and the frequency of adverse events, and the change from baseline for a variety of serum, imaging, and electrophysiological measurements. We also studied the overall distribution of disability scores on the modified Rankin Scale (mRS) and the Barthel index.</p> <p><b>Results:</b> We treated 300 patients with NXY-059 and 303 with placebo. Treatment groups were well matched for prognostic variables including Glasgow Coma Scale, risk factors, and age. The mean National Institute of Health Stroke Scale score on admission was 14 in both groups. The baseline hemorrhage volume was 22.4±20.1 mL in the NXY-059 group and 23.3±22.8 mL in the placebo group (mean±SD). Most hemorrhages were related to hypertension or anticoagulant use. Mortality was similar in both groups: 20.3% for NXY-059 and 19.8% for placebo-treated patients. The proportion of patients who experienced an adverse event was the same for both groups, whereas for serious adverse events the proportion was slightly higher in the NXY-059 group. However, no pattern emerged to indicate a safety concern. Serum potassium fell transiently in both groups, lower in the NXY-059 group. There were no differences in 3-month function, disability, or neurological deficit scores. The odds ratio for an improved outcome in 3-month mRS scores in the NXY-059 group was 1.01 (95% CI 0.75, 1.35).</p> <p><b>Conclusions:</b> NXY-059 given within 6 hours of acute ICH has a good safety and tolerability profile, with no adverse effect on important clinical outcomes.</p&gt

CT imaging of dogs with perineal hernia reveals large prostates with morphological and spatial abnormalities

The etiology of canine perinea) hernia (PH) remains unclear, although as a disease of older male dogs, it is likely to be hormonal. The role of the prostate in the formation of PH has been questioned; however, prospective and systematic evaluation of prostates in these dogs is absent in the literature. In this prospective case-control study, CT imaging was used to assess prostatic changes in dogs with PH (n = 46) and compare these findings with those of intact age-matched male dogs (n = 23). Using the OsiriX (R) DICOM viewer, we measured prostatic volume and correlated it with the size of the dog by using the length of the sixth lumbar vertebra. In addition, we recorded spatial and morphological changes of the prostate, such as heterogenicity, intra- and paraprostatic cysts, and mineralizations, as well as prostatic location and rotation. We found that dogs with PH had larger prostates (P < .001) that more often contained cysts (P < .001) and had larger cyst diameters (P = .013) than age-matched controls. Prostates of PH dogs also contained paraprostatic cysts (17.4%) and focal mineralizations (32.6%), which were absent in the control group. Abnormal rotation and location of the prostate were common in dogs with PH. In conclusion, these findings support the use of CT as an adjunct diagnostic imaging modality for the evaluation of the prostate in dogs with PH. Further studies are needed to evaluate nonprostatic CT findings in the pelvic cavity of PH dogs.Peer reviewe

Tides: A key environmental driver of osteichthyan evolution and the fish-tetrapod transition?

Tides are a major component of the interaction between the marine and terrestrial environments, and thus play an important part in shaping the environmental context for the evolution of shallow marine and coastal organisms. Here, we use a dedicated tidal model and palaeogeographic reconstructions from the Late Silurian to early Late Devonian (420 Ma, 400 Ma and 380 Ma, Ma = millions of years ago) to explore the potential significance of tides for the evolution of osteichthyans (bony fish) and tetrapods (land vertebrates). The earliest members of the osteichthyan crown-group date to the Late Silurian, approximately 425 Ma, while the earliest evidence for tetrapods is provided by trackways from the Middle Devonian, dated to approximately 393 Ma, and the oldest tetrapod body fossils are Late Devonian, approximately 373 Ma. Large tidal ranges could have fostered both the evolution of air-breathing organs in osteichthyans to facilitate breathing in oxygen-depleted tidal pools, and the development of weight-bearing tetrapod limbs to aid navigation within the intertidal zones. We find that tidal ranges over 4 m were present around areas of evolutionary significance for the origin of osteichthyans and the fish-tetrapod transition, highlighting the possible importance of tidal dynamics as a driver for these evolutionary processes

Rare earth elements (REEs) in vertebrate microremains from the upper Pridoli Ohesaare beds of Saaremaa Island, Estonia: geochemical clues to palaeoenvironment c

This is an open access article, available to all readers online, licensed under the Creative Commons Attribution 4.0 International License (CC BY; http://creativecommons.org/licenses/by/4.0/). The attached file is the published version of the article