9 research outputs found
Relationship between the magnitude of intraocular pressure during an episode of acute elevation and retinal damage four weeks later in rats
PURPOSE: To determine relationship between the magnitude of intraocular pressure (IOP) during a fixed-duration episode of acute elevation and the loss of retinal function and structure 4 weeks later in rats. METHODS: Unilateral elevation of IOP (105 minutes) was achieved manometrically in adult Brown Norway rats (9 groups; n = 4 to 8 each, 10-100 mm Hg and sham control). Full-field ERGs were recorded simultaneously from treated and control eyes 4 weeks after IOP elevation. Scotopic ERG stimuli were white flashes (-6.04 to 2.72 log cd.s.m(-2)). Photopic ERGs were recorded (1.22 to 2.72 log cd.s.m(-2)) after 15 min of light adaptation (150 cd/m(2)). Relative amplitude (treated/control, %) of ERG components versus IOP was described with a cummulative normal function. Retinal ganglion cell (RGC) layer density was determined post mortem by histology. RESULTS: All ERG components failed to recover completely normal amplitudes by 4 weeks after the insult if IOP was 70 mmHg or greater during the episode. There was no ERG recovery at all if IOP was 100 mmHg. Outer retinal (photoreceptor) function demonstrated the least sensitivity to prior acute IOP elevation. ERG components reflecting inner retinal function were correlated with post mortem RGC layer density. CONCLUSIONS: Retinal function recovers after IOP normalization, such that it requires a level of acute IOP elevation approximately 10 mmHg higher to cause a pattern of permanent dysfunction similar to that observed during the acute event. There is a 'threshold' for permanent retinal functional loss in the rat at an IOP between 60 and 70 mmHg if sustained for 105 minutes or more
Representative ERG findings for experimental eyes (bold traces) 4 weeks after challenge for all IOP levels.
<p>Selected stimulus conditions are shown to emphasize various components of the ERG: scotopic threshold response (STR, −5.55 log cd s m<sup>−2</sup>, Column <b>A</b>) the scotopic bright flash ERG (2.22 log cd s m<sup>−2</sup>, Column <b>B</b>) and OPs (Column <b>C)</b>, as well as the photopic ERG (2.72 log cd s m<sup>−2</sup>, Column <b>D</b>) and Ops (Column <b>E</b>). ERG responses for fellow control eyes are shown by the thin traces in each column. IOP levels are indicated on the left.</p
Cumulative normal parameters for relative amplitude as a function of acute IOP.
*<p>significant recovery p<0.05 compared with acute from non-parametric bootstrap.</p
Representative individual examples of ERG findings 4 weeks after acute IOP elevation for selected groups.
<p>ERG responses for experimental eyes (bold traces) and their fellow control eyes (thin traces) are shown for the following groups: sham (Column <b>A</b>), 70 mmHg (Column <b>B</b>), 80 mmHg (Column <b>C</b>) and 100 mmHg (Column <b>D</b>). Stimulus flash intensities are listed at left for scotopic (below) and photopic (above) responses. Isolated OPs are shown to the right of corresponding waveforms.</p
Recovery of ERG components as a function of stimulus intensity.
<p>Averaged group (± SEM) data for control eyes (unfilled symbols) and experimental eyes (filled symbols).</p
Relative change across ERG components 4 weeks following acute IOP insult.
<p>(<b>A</b>) nSTR (filled triangles), P2 amplitude (V<i><sub>max</sub></i>, open circles) and photoreceptoral amplitude (Rm<i><sub>P3</sub></i>, filled squares). For comparison, the corresponding best-fit cumulative normal functions are also plotted in panel A for the nSTR (thin solid curve), P2 (V<i><sub>max</sub></i>, dashed curve) and a-wave (Rm<i><sub>P3</sub></i>, bold curve). (<b>B</b>) Scotopic OP amplitude. (<b>C</b>) Photopic b-wave amplitude (V<i><sub>max</sub></i>, open diamonds) and photopic OP amplitude (filled diamonds). For clarity, the best-fit cumulative normal functions are omitted from panels B and C.</p
ERG component amplitudes recorded during acute IOP elevation vs.
<p><b>after 4 weeks recovery.</b> Group mean relative amplitude (ratio of treated/fellow control eyes, %, filled symbols, ± SEM) for (<b>A</b>) a-wave (Rm<i><sub>P3</sub></i>, P3), (<b>B</b>) b-wave (V<i><sub>max</sub></i>, P2), (<b>C</b>) nSTR, (<b>D</b>) nSTR, (<b>E</b>) Photopic b-wave (V<i><sub>max</sub></i>, P2) and (<b>F</b>) photopic OP components 4 weeks following acute IOP elevation for each level of IOP (solid curves represent best-fit cumulative normal functions). For comparison, the relative amplitudes of each component recorded during acute IOP elevation are re-plotted from a previous report <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070513#pone.0070513-Bui1" target="_blank">[20]</a> (unfilled symbols, dashed curves represent best-fit cumulative normal functions).</p