Changes in muscle strength, muscle fibre size and myofibrillar gene expression after immobilization and retraining in humans

Changes in muscle strength, vastus lateralis fibre characteristics and myosin heavy-chain (MyoHC) gene expression were examined in 48 men and women following 3 weeks of knee immobilization and after 12 weeks of retraining with 1866 eccentric, concentric or mixed contractions.Immobilization reduced eccentric, concentric and isometric strength by 47 %. After 2 weeks of spontaneous recovery there still was an average strength deficit of 11 %. With eccentric and mixed compared with concentric retraining the rate of strength recovery was faster and the eccentric and isometric strength gains greater.Immobilization reduced type I, IIa and IIx muscle fibre areas by 13, 10 and 10 %, respectively and after 2 weeks of spontaneous recovery from immobilization these fibres were 5 % smaller than at baseline. Hypertrophy of type I, IIa and IIx fibres relative to baseline was 10, 16 and 16 % after eccentric and 11, 9 and 10 % after mixed training (all P < 0.05), exceeding the 4, 5 and 5 % gains after concentric training. Type IIa and IIx fibre enlargements were greatest after eccentric training.Total RNA/wet muscle weight and type I, IIa and IIx MyoHC mRNA levels did not change differently after immobilization and retraining. Immobilization downregulated the expression of type I MyoHC mRNA to 0.72-fold of baseline and exercise training upregulated it to 0.95 of baseline. No changes occurred in type IIa MyoHC mRNA. Immobilization and exercise training upregulated type IIx MyoHC mRNA 2.9-fold and 1.2-fold, respectively. For the immobilization segment, type I, IIa and IIx fibre area and type I, IIa and IIx MyoHC mRNA correlated (r = 0.66, r = 0.07 and r = −0.71, respectively).The present data underscore the role muscle lengthening plays in human neuromuscular function and adaptation

Precipitation and Evaporation Budgets over the Baltic Proper: Observations and Modelling

Precipitation and evaporation budgets over the Baltic Sea were studied in a concerted project called PEP in BALTEX (Pilot study of Evaporation and Precipitation in the Baltic Sea), combining extensive field measurements and modelling efforts. Eddy-correlation-measurements of turbulent heat flux were made on a semi-continuous basis for a 12 month period at four well-exposed coastal sites in the Baltic Proper (the main basin of the Baltic Sea). Precipitation was measured at land-based sites with standard gauges and on four merchant ships travelling between Germany and Finland with the aid of specially designed ship rain gauges (SRGs). The evaporation and precipitation regime of the Baltic Sea was modelled for a 12 month period by applying a wide range of numerical models: the operational atmospheric High Resolution Limited Area Model (HIRLAM, Swedish and Finnish versions), the German atmospheric REgional-scale MOdel, REMO, the operational German Europe Model (only precipitation), the oceanographic model PROBE-Baltic, and two models that use interpolation of ground-based data, the Swedish MESAN model of SMHI and a German model of IFM-GEOMAR Kiel. Modelled precipitation was compared with SRG measurements on board the ships. A reasonable correlation was obtained, but the regional-scale models and MESAN gave some 20% higher precipitation over the sea than is measured. Bulk parameterisation schemes for evaporation were evaluated against measurements. A constant value of C HN and C EN with wind speed, underestimated large fluxes of both sensible and latent heat flux. The limited area models do not resolve the influence of the height of the marine boundary layer in coastal zones and the entrainment (on the surface fluxes), which may explain the observed low correlations between modelled and measured latent heat fluxes. Estimates of evaporation, E, and precipitation, P, for the entire Baltic Proper were made with several models for a 12 month period. While the annual variation was well represented by all predictions, there are still important differences in the annual means. Evaporation ranges from 509 to 625 mm year−1 and precipitation between 624 and 805 mm year−1 for this particular 12 month period. Taking the results of model verification from the present study into account, the best estimate of P–E is about 100 ± 50 mm for this particular 12 month period. But the annual mean of P–E varies considerably from year to year. This is reflected in simulations with the PROBE-Baltic model for an 18 year period, which gave 95 mm year−1 for the 12 month period studied here and 32 mm year−1 as an average for 18 years