Panorama fiscal de América Latina y el Caribe: Año 2014

The rapid and continuing progress in gene discovery for complex diseases is fuelling interest in the potential application of genetic risk models for clinical and public health practice. The number of studies assessing the predictive ability is steadily increasing, but they vary widely in completeness of reporting and apparent quality. Transparent reporting of the strengths and weaknesses of these studies is important to facilitate the accumulation of evidence on genetic risk prediction. A multidisciplinary workshop sponsored by the Human Genome Epidemiology Network developed a checklist of 25 items recommended for strengthening the reporting of Genetic RIsk Prediction Studies (GRIPS), building on the principles established by prior reporting guidelines. These recommendations aim to enhance the transparency, quality and completeness of study reporting, and thereby to improve the synthesis and application of information from multiple studies that might differ in design, conduct or analysis

A road map for efficient and reliable human genome epidemiology

Networks of investigators have begun sharing best practices, tools and methods for analysis of associations between genetic variation and common diseases. A Network of Investigator Networks has been set up to drive the process, sponsored by the Human Genome Epidemiology Network. A workshop is planned to develop consensus guidelines for reporting results of genetic association studies. Published literature databases will be integrated, and unpublished data, including 'negative' studies, will be captured by online journals and through investigator networks. Systematic reviews will be expanded to include more meta-analyses of individual-level data and prospective meta-analyses. Field synopses will offer regularly updated overviews. \ua9 2006 Nature Publishing Group

Radio Pulsars

Almost 50 years after radio pulsars were discovered in 1967, our understanding of these objects remains incomplete. On the one hand, within a few years it became clear that neutron star rotation gives rise to the extremely stable sequence of radio pulses, that the kinetic energy of rotation provides the reservoir of energy, and that electromagnetic fields are the braking mechanism. On the other hand, no consensus regarding the mechanism of coherent radio emission or the conversion of electromagnetic energy to particle energy yet exists. In this review, we report on three aspects of pulsar structure that have seen recent progress: the self-consistent theory of the magnetosphere of an oblique magnetic rotator; the location, geometry, and optics of radio emission; and evolution of the angle between spin and magnetic axes. These allow us to take the next step in understanding the physical nature of the pulsar activity.Comment: Invited review for Space Science Review

Axial resolution enhancement by 4Pi confocal fluorescence microscopy with two-photon excitation

Confocal fluorescence microscopy and two-photon microscopy have become important techniques for the three-dimensional imaging of intact cells. Their lateral resolution is about 200–300 nm for visible light, whereas their axial resolution is significantly worse. By superimposing the spherical wave fronts from two opposing objective lenses in a coherent fashion in 4Pi microscopy, the axial resolution is greatly improved to ?100 nm. In combination with specific tagging of proteins or other cellular structures, 4Pi microscopy enables a multitude of molecular interactions in cell biology to be studied. Here, we discuss the choice of appropriate fluorescent tags for dual-color 4Pi microscopy and present applications of this technique in cellular biophysics. We employ two-color fluorescence detection of actin and tubulin networks stained with fluorescent organic dyes; mitochondrial networks are imaged using the photoactivatable fluorescent protein EosFP. A further example concerns the interaction of nanoparticles with mammalian cells. <br/