Variants in taste genes on caries risk and caries activity status

WOS: 000557110000001PubMed: 32770263The aim of this study was to evaluate the role of taste-related gene polymorphisms (CA6,TAS1R1,TAS1R3, TLR2,andTLR4)on dental caries and caries activity in adults. Individuals aged 25-44 years included in the study were assigned to two groups according to the decayed-missing-filled teeth index (DMFT) as the high caries risk (DMFT >= 14,n = 100) and the low caries risk (DMFT <= 5,n = 100). TaqMan allelic discrimination assays were used for genotyping the gene variants after isolating the DNA from the buccal smears. According to the American dental association caries classification system (ADA CCS), all teeth were scored as initial, moderate or advanced caries. the variant of the gustin (CA6) in saliva was found to be associated with a high caries risk (CA6rs17032907,P < .001). There was also a statistically significant difference in the dominant model of the same variant (CC vs. TT:P < .001, OR = 5.05, 95% CI: 2.38-10.71). the presence of genotype CC and allele C was less frequent in the advanced caries lesion group (P < .001). This study shows that theCA6rs17032907 gene variant may be a risk factor for dental caries affecting caries activity. Clinical Trials ID: NCT04066101.Recep Tayyip Erdogan University Scientific Research Projects Foundation [TSA-2017-772]The study was supported by the Recep Tayyip Erdogan University Scientific Research Projects Foundation (TSA-2017-772)

Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

Testicular ischaemia reperfusion (I/R) injury results with serious dysfunctions in testis. This study aims to explore effects of soluble guanylate cyclase (sGC) stimulator Riociguat on experimental testicular I/R injury in rats. Twenty-one male rats were divided into three groups (Control, IR and IRR). The control group was not exposed to any application. Bilateral testis from IR and IRR animals were rotated 720 degrees in opposite directions for 3 h to induce experimental testicular ischaemia. Animals in IR and IRR groups were subjected to 3 h of reperfusion. Isotonic and Riociguat were administered to the animals 30 min prior reperfusion by oral gavage. At the end of experiment, animals were sacrificed and tissue samples were used for analyses. Riociguat treatment significantly decreased tissue malondialdehyde and Luminol levels compared to the IR group (p < 0.05). The pathological changes, pro-apoptotic proteins (Bax, Caspase 3, and Caspase 9) and apoptotic index in the IR group were down regulated in Riociguat treated animals (p < 0.05). Riociguat treatment was also significantly increased anti-apoptotic Bcl-2 expression, but alleviated tissue injury via modulating pro-inflammatory cytokine IL-1 beta levels and significantly (p < 0.05) down-regulating NF-kappa B activity. Moreover, mTOR and ERK phosphorylation increased in IR group (p < 0.05), but Riociguat treatment reduced protein phosphorylation. Our experiment indicated that targeting sGC might support surgical interventions in testicular I/R injury by modulating oxidative stress, inflammation, and apoptotic protein expression levels, but more detailed studies are required to explore the protective activity of Riociguat and underlying mechanisms in testicular I/R injury