Cancer Borealis Stomatogastric Nervous System Dissection

The stomatogastric ganglion (STG) is an excellent model for studying cellular and network interactions because it contains a relatively small number of cells (approximately 25 in C. borealis) which are well characterized. The cells in the STG exhibit a broad range of outputs and are responsible for the motor actions of the stomach. The stomach contains the gastric mill which breaks down food with three internal teeth, and the pylorus which filters the food before it reaches the midgut. The STG produces two rhythmic outputs to control the gastric mill and pylorus known as central pattern generators (CPGs). Each cell in the STG can participate in one or both of these rhythms. These CPGs allow for the study of neuromodulation, homeostasis, cellular and network variability, network development, and network recovery

Propuesta de un plan integrado de marketing de turismo endógeno para la Comuna de Chanco en el período no estival

Chanco, presenta un gran potencial turí stico, destacando atracciones naturales, culturales, religiosas y arquitectura colonial. La Municipalidad, privados y comunidad local actualmente trabajan para difundir el turismo, pero de manera independiente, potenciando sólo actividades para el perí odo estival. No existen actividades que permitan atraer al turista no estival, siendo necesario diseñar una propuesta de un plan de marketing, detectando la oferta turística mediante entrevistas en profundidad con la Municipalidad, SERNATUR y expertos. Para analizar la demanda turí stica efectiva se aplicó una encuesta utilizando un cuestionario bilingüe como instrumento de medición, aplicado en el perí odo no estival a los turistas que visitaron la comuna de Chanco desde el 6 al 30 de septiembre de 2004, el muestreo fue por conveniencia. Los lugares de aplicación del cuestionario fueron: Reserva Nacional Federico Albert, Reserva Nacional Los Ruiles, Plaza de Armas y Fundo San Bonifacio, se entrevistaron a 96 personas. Los resultados obtenidos fueron procesados con el software estadístico SPSS 11.5 y Excel 2000. El segmento de mercado meta actual identificado corresponde a aquellos turistas que han visitado anteriormente la comuna, su principal motivación es visitar a parientes o amigos, las actividades que realizan son visitar playas, su nivel de ingresos está entre $115.000 y$500.000. Consideran bueno el servicio de hospedaje y malo el servicio de restaurante, la atención en los atractivos turí sticos no es considerada buena, el sistema señalético, no es visible, mal diseñado y ambiguo, el transporte es mal evaluado por los turistas. Los turistas tienen una opinión favorable respecto a la artesaní a, un 40 % compra queso y frutillas. Los principales canales de distribución son SERNATUR y Oficinas de Información Turística de otras Municipalidades, pero un canal de la Municipalidad eficaz es el método de recomendación “boca a boca”. Internet es otro medio mediante el cual los turistas se han enterado de la comuna, especí ficamente los visitantes del Fundo San Bonifacio. La eestrategia se definió a través del establecimiento de la comunicación directa entre el producto turí stico definido y los turistas. Las entidades locales y Municipalidad deben diseñar trí pticos y folletos distribuidos a la V, VI, VII, VIII Región y Metropolitana, mediante SERNATUR y Oficinas de Información Turí stica de las Municipalidades. Además publica

Maternal-child consumption of ultra-processed foods and sugar-sweetened beverages in informal settlements in Mumbai, India.

BACKGROUND: The global nutrition transition is associated with increased consumption of ultra-processed snack foods and sugar-sweetened beverages (UPF/SSB), contributing to the double burden of child obesity and undernutrition. METHODS: This cross-sectional study describes the prevalence of maternal and child UPF/SSB consumption and the factors associated with frequent consumption in a convenience sample of 749 children ages 6 months through 6 years and their mothers participating in a community-based child oral health program in five informal settlement communities in Mumbai, India. Mothers were interviewed regarding maternal and child oral health and nutrition characteristics, including consumption of beverages and foods associated with tooth decay-milk, soda, tea with sugar, sweets, and chips/biscuits-using standardized questionnaires. Spearman correlations were used to assess for associations between various social factors and the frequency of maternal and child consumption of the five food categories. Chi-square tests were used to assess differences in child consumption patterns by age groups. RESULTS: Though reported soda consumption was low among both mothers and children, nearly 60% of children consumed sweets and chips/biscuits daily, four to five times the rate of mothers. Factors associated with childrens frequent consumption of UPF/SSB included lower maternal education level, frequent maternal consumption of UPF/SSB, greater number of household members, greater amount of money given to the child, and closer proximity to a store. CONCLUSION: Our findings demonstrate social factors that may promote UPF/SSB consumption. The nutritional dangers of sugary drinks and non-nutritious snacks for mothers and young children should be addressed across maternal-child health, education, and social service programs. Early childhood nutrition interventions should involve the entire family and community and emphasize the need to limit childrens consumption of unhealthy foods and beverages from an early age

Influence of depression and interpersonal support on adherence to antiretroviral therapy among people living with HIV

BackgroundPoor adherence and under-utilization of antiretroviral therapy (ART) services have been major setbacks to achieving 95-95-95 policy goals in Sub-Saharan Africa. Social support and mental health challenges may serve as barriers to accessing and adhering to ART but are under-studied in low-income countries. The purpose of this study was to examine the association of interpersonal support and depression scores with adherence to ART among persons living with HIV (PLWH) in the Volta region of Ghana.MethodsWe conducted a cross-sectional survey among 181 PLWH 18 years or older who receive care at an ART clinic between November 2021 and March 2022. The questionnaire included a 6-item simplified ART adherence scale, the 20-item Center for Epidemiologic Studies Depression Scale (CES-D), and the 12-item Interpersonal Support Evaluation List-12 (ISEL-12). We first used a chi-squared or Fisher’s exact test to assess the association between these and additional demographic variables with ART adherence status. We then built a stepwise multivariable logistic regression model to explain ART adherence.ResultsART adherence was 34%. The threshold for depression was met by 23% of participants, but it was not significantly associated with adherence in multivariate analysis(p = 0.25). High social support was reported by 48.1%, and associated with adherence (p = 0.033, aOR = 3.45, 95% CI = 1.09–5.88). Other factors associated with adherence included in the multivariable model included not disclosing HIV status (p = 0.044, aOR = 2.17, 95% CI = 1.03–4.54) and not living in an urban area (p = 0.00037, aOR = 0.24, 95% CI = 0.11–0.52).ConclusionInterpersonal support, rural residence, and not disclosing HIV status were independent predictors of adherence to ART in the study area

Dysregulated lipid metabolism networks modulate T-cell function in people with relapsing-remitting multiple sclerosis

Altered cholesterol, oxysterol, sphingolipid, and fatty acid concentrations are reported in blood, cerebrospinal fluid, and brain tissue of people with relapsing remitting multiple sclerosis (RRMS) and are linked to disease progression and treatment responses. CD4+ T cells are pathogenic in RRMS, and defective T cell function could be mediated in part by liver X receptors (LXRs) - nuclear receptors that regulate lipid homeostasis and immunity. RNA-sequencing and pathway analysis identified that genes within the 'lipid metabolism' and 'signalling of nuclear receptors' pathways were dysregulated in CD4+ T cells isolated from RRMS patients compared with healthy donors. While LXRB and genes associated with cholesterol metabolism were upregulated, other T cell LXR-target genes, including genes involved in cellular lipid uptake (inducible degrader of the LDL receptor, IDOL), and the rate-limiting enzyme for glycosphingolipid biosynthesis (UDP-glucosylceramide synthase, UGCG) were downregulated in T cells from patients with RRMS compared to healthy donors. Correspondingly, plasma membrane glycosphingolipids were reduced, and cholesterol levels increased in RRMS CD4+ T cells, an effect partially recapitulated in healthy T cells by in vitro culture with T cell receptor stimulation in the presence of serum from RRMS patients. Notably, stimulation with LXR-agonist GW3965 normalised membrane cholesterol levels, and reduced proliferation and IL17 cytokine production in RRMS CD4+ T-cells. Thus, LXR-mediated lipid metabolism pathways were dysregulated in T cells from patients with RRMS and could contribute to RRMS pathogenesis. Therapies that modify lipid metabolism could help restore immune cell function

Identifying Novel Biomarkers Ready for Evaluation in Low-Prevalence Populations for the Early Detection of Upper Gastrointestinal Cancers : A Systematic Review

Funding This study and the journal’s rapid service fee were supported by the CanTest Collaborative (funded by Cancer Research UK C8640/A23385) of which Fiona M. Walter is Director, Jon Emery is an Associate Director, Mike Messenger is co-investigator, and Natalia Calanzani and Garth Funston are researchers. The funder of the study had no role in study design, data collection, data analysis, data interpretation, or writing of the report. Paige Druce, Kristi Milley and Jon Emery are supported by the Cancer Australia Primary Care Collaborative Cancer Clinical Trials Group (PC4). Mike Messenger is funded by the NIHR Leeds In Vitro Diagnostic Co-operative (UK). No Open Access Fee was received by the journal for the publication of this article.Peer reviewedPublisher PD

Gargantuan hail in Argentina

On 8 February 2018, a supercell storm produced gargantuan (>15 cm or >6 in. in maximum dimension) hail as it moved over the heavily populated city of Villa Carlos Paz in Córdoba Province, Argentina. Observations of gargantuan hail are quite rare, but the large population density here yielded numerous witnesses and social media pictures and videos from this event that document multiple large hailstones. The storm was also sampled by the newly installed operational polarimetric C-band radar in Córdoba. During the RELAMPAGO campaign, the authors interviewed local residents about their accounts of the storm and uncovered additional social media video and photographs revealing extremely large hail at multiple locations in town. This article documents the case, including the meteorological conditions supporting the storm (with the aid of a high-resolution WRF simulation), the storm's observed radar signatures, and three noteworthy hailstones observed by residents. These hailstones include a freezer-preserved 4.48-in. (11.38 cm) maximum dimension stone that was scanned with a 3D infrared laser scanner, a 7.1-in. (18 cm) maximum dimension stone, and a hailstone photogrammetrically estimated to be between 7.4 and 9.3 in. (18.8-23.7 cm) in maximum dimension, which is close to or exceeds the world record for maximum dimension. Such a well-observed case is an important step forward in understanding environments and storms that produce gargantuan hail, and ultimately how to anticipate and detect such extreme events.Fil: Kumjian, Matthew R.. State University of Pennsylvania; Estados UnidosFil: Gutierrez, Rachel. State University of Pennsylvania; Estados UnidosFil: Soderholm, Joshua S.. Universitat Bonn; AlemaniaFil: Nesbitt, Stephen William. University of Illinois at Urbana; Estados UnidosFil: Maldonado, Paula Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Luna, Lorena Medina. National Center for Atmospheric Research; Estados UnidosFil: Marquis, James. Pacific Northwest National Laboratory; Estados UnidosFil: Bowley, Kevin A.. State University of Pennsylvania; Estados UnidosFil: Alvarez Imaz, María de Los Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Salio, Paola Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentin

Gargantuan Hail Documenting an Extreme Forecasting Challenge

I n the scientific literature, some studies have identified “giant” hail as those stones with maximum dimensions exceeding 10 cm or 4 in. We propose a new size class for hailstones with maximum dimensions exceeding 15 cm or 6 in. (referred to here as “gargantuan hail”) to represent the upper extreme of hail sizes. As these are rare cases, only a few studies have specifically documented giant and gargantuan hail events, and most are individual case studies. One study of giant and gargantuan hailstones from the Aurora, Nebraska, storm of 2003 showed that every stone evaluated exhibited an outer (i.e., final) growth layer indicating wet growth, and in some cases this layer was of quite substantial thickness. Another study used social media reports to identify giant hail that ultimately became certified state records. It suggested that the occurrence of giant or gargantuan hail is significantly underreported. None of the aforementioned studies focused on observedtorm properties or environments. In con- trast, a 2013 study documented the synoptic and mesoscale environment of the super- cell that produced the Vivian, South Dakota, hailstone (which registers as the world re- cord for maximum dimension of 20 cm or 8 in.). Analysis indicated that the environment was indeed supportive of severe convective storms, but not indicative of such large hail as was observedFil: Kumjian, Matthew R.. State University of Pennsylvania; Estados UnidosFil: Gutierrez, Rachel. State University of Pennsylvania; Estados UnidosFil: Soderholm, Joshua S.. State University of Pennsylvania; Estados UnidosFil: Nesbitt, Stephen William. University of Illinois at Urbana; Estados UnidosFil: Maldonado, Paula Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Medina Luna, Lorena. National Center for Atmospheric Research; Estados UnidosFil: Marquis, James. Pacific Northwest National Laboratory; Estados UnidosFil: Bowley, Kevin A.. State University of Pennsylvania; Estados UnidosFil: Alvarez Imaz, María de Los Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Salio, Paola Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentin

Daily fraction dose recalculation based on rigid registration using Cone Beam CT

Purpose: To calculate the daily fraction dose for CBCT recalculations based on rigid registration and compare it to the planned CT doses.Methods: For this study, 30 patients that were previously treated (10 SBRT lung, 10 prostate and 10 abdomen) were considered. The daily CBCT images were imported into the Pinnacle treatment planning system from Mosaic. Pinnacle was used to re-contour the regions of interest (ROI) for the specific CBCT by copying the contours from the original CT plan, planned by the prescribing physician, onto each daily CBCT and then manually reshaping contours to match the ROIs. A new plan is then created with the re-contoured CBCT as primary image in order to calculate the daily dose delivered to each ROI. The DVH values are then exported into Excel and overlaid onto the original CT DVH to produce a graph.Results: For the SBRT lung patients, we found that there were small daily volume changes in the lungs, trachea and esophagus. For almost all regions of interest we found that the dose received each day was less than the predicted dose of the planned CT while the PTV dose was relatively the same each day. The results for the prostate patients were similar, showing slight differences in the DVH values for different days in the rectum and bladder but similar PTV.Conclusion: By comparing daily fraction dose between the re-contoured CBCT images and the original planned CT show that PTV coverage for both prostate and SBRT, it has been shown that for PTV coverage, a planned CT is adequate. However, there are differences between the dose for the organs surrounding the PTV. The dose difference is less than the planned in most instances.-----------------------Cite this article as: Bosse C, Tuohy R, Mavroidis P, Shi Z, Crownover R, Gutierrez A, Papanikolaou N, Stathakis S. Daily fraction dose recalculation based on rigid registration using Cone Beam CT. Int J Cancer Ther Oncol 2014; 2(2):020217. DOI: 10.14319/ijcto.0202.1

Global Kidney Exchange: Analysis and Background Papers from the Perspective of the Right to Health

Global Kidney Exchange (GKE) is a program aimed at facilitating trans-national kidney donation. Although its proponents aim at reducing the unmet demand of kidneys in the United States through the trans-nationalization of kidney exchange programs, the World Health Organization (WHO) and The Transplantation Society (TTS) have expressed concerns about its potential effect on black markets of organs and transnational organ trafficking, as well as on low- or middle-income countries health systems. For GKE to be implemented, it would need to be permitted to operate in at least some low- or middle-income countries. What are the right to health implications of GKE’s implementation? With the aim of answering this question, the eighteen University of Denver students in the First Year Seminar course I taught in autumn 2017 with the title “The Right to Health in Theory and Practice”, identified and researched the different aspects that would affect this issue, and produced the analysis we present in this report. Based on our analysis, the potential right to health implications of GKE are: First, the program may improve timely access to organ donation primarily to patients with health insurance in the United States. Second, a large-scale implementation of the program may have a positive impact on health costs savings, which potentially could benefit the United States health system. Third, on a global health level, the program relies on existing health inequalities among countries in terms of funding, human resources, and health system strengthening, and it is likely to exacerbate those inequalities. Fourth, the program has the potential of negatively affecting the efforts that low- and middle-income countries are already doing to address end-stage renal failure, including the improvement of their own organ donation systems. Finally, given what we have learned about the current situation of organ trafficking, it is easy to think that GKE would unintentionally end up being linked to chains of organ trade. The only way how a program like GKE could have a positive impact from a right to health perspective is if it establishes local partnerships that have the effect of decreasing health inequalities. Additionally, we identified some issues of concern that are beyond the level of influence of local authorities: the unmet demand of kidneys in high-income countries is a reality that incentivizes organ trade and transplant tourism, and this is a problem in need of solutions; transnational organ trafficking as well as human trafficking with the purpose of organ donation are problems that need more visibility; for a global exchange of organs to be implemented, it would need to rely on supranational or transnational regulation and oversight; and the global epidemic of chronic kidney disease needs to be addressed through a public health perspective that emphasizes prevention