First histopathological study in kidneys of rodents naturally infected with Leptospira pathogenic species from Yucatan, Mexico

AbstractObjectiveTo report the renal histological lesions in synanthropic rodents, Mus musculus and Rattus rattus, naturally infected with Leptospira spp., captured in a rural community in Yucatan, Mexico.MethodsKidney samples of synanthropic rodents were collected from a rural community in Yucatan, Mexico. Polymerase chain reaction was used to detect Leptospira spp. infection. Tissue kidney was fixed in 10% buffered formalin, processed according to the usual techniques for paraffin inclusion, cut and stained with hematoxylin and eosin, and examined using a conventional electronic microscope.ResultsA total of 187 rodents were captured. Nine individuals (4.8%) were positive for Leptospira spp. in the molecular analysis. All renal lesions observed in the histopathological study had been reported previously for Leptospira spp. infection.ConclusionsThe histopathological lesions are present in the kidneys, plus the results of the polymerase chain reaction confirm that these rodents are true carriers of Leptospira spp

MOLECULAR SURVEY OF Ehrlichia canis IN DOGS FROM MEXICO: PREVALENCE OF INFECTION AND POSSIBLE ASSOCIATED FACTORS/Estudio molecular de Ehrlichia canis en perros de México: prevalencia de infección y posibles factores asociados

. A cross-sectional study was performed to estimate the prevalence of infection of Ehrlichia canis in dogs and to explore the factors associated with the presence of the bacteria. The study was carried out in a village in Yucatan, Mexico. Blood samples were obtained from 200 dogs. Samples were analyzed by nested-PCR to detect the presence of E. canis DNA and thrombocyte counts were calculated. One-hundred and forty of the dogs (70 %) were found to be infested with ticks. A total of 1 116 ticks were recovered and all were identied as Rhipicephalus sanguineus. The prevalence of E. canis infection was 69.2 %. None of the variables studied (gender, age, body condition, platelet-related bleeding, thrombocytopenia, and presence of ticks) showed association with E. canis infection. In conclusion, there is a high probability that dogs living in Yucatan, Mexico are infected with E. canis

Acción de repetición en Colombia: ¿Eficaz o Ineficaz?

Este trabajo producto de los resultados y del análisis de la Investigación desarrollada entre 2008 y 2009, pretende definir los factores que inciden en la ineficacia de la acción de repetición, acción que fue constituida como herramienta del derecho administrativo para reponer al erario público, el dinero pagado por condenas al Estado, con conductas dolosas o gravemente culposas de sus agentes. Fallas en su procedimiento, en la presentación y desarrollo del debate probatorio, vacíos sustanciales en la norma, e incidencia de la cultura a falta de dolientes al Estado, son algunos de los factores identificados en la investigación que determinan la falta de eficacia y el no cumplimiento de la finalidad de la acción de repetición, aspectos que se abordan en el presente estudio

Molecular survey of Ehrlichia canis in dogs from Mexico: prevalence of infection and possible associated factors

Abstract: A cross-sectional study was performed to estimate the prevalence of infection of Ehrlichia canis in dogs and to explore the factors associated with the presence of the bacteria. The study was carried out in a village in Yucatan, Mexico. Blood samples were obtained from 200 dogs. Samples were analyzed by nested-PCR to detect the presence of E. canis DNA and thrombocyte counts were calculated. One-hundred and forty of the dogs (70 %) were found to be infested with ticks. A total of 1 116 ticks were recovered and all were identified as Rhipicephalus sanguineus. The prevalence of E. canis infection was 69.2 %. None of the variables studied (gender, age, body condition, platelet-related bleeding, thrombocytopenia, and presence of ticks) showed association with E. canis infection. In conclusion, there is a high probability that dogs living in Yucatan, Mexico are infected with E. canis.Resumen: Un estudio de sección cruzada fue realizada para estimar la prevalencia de infección de Ehrlichia canis en perros y explorar la presencia de factores asociados con la presencia de la bacteria. El estudio se realizó en una comunidad de Yucatán, México. Muestras de sangre de un total de 200 perros fueron obtenidas. Las muestras fueron analizadas por Nested-PCR para detectar la presencia de ADN de E. canis y se calculó el conteo de Plaquetas. 140 perros de 200 estudiados (70 %) estuvieron infestados por garrapatas. Un total de 1 116 garrapatas fueron recuperadas y todas identificadas como Rhipicephalus sanguineus. La prevalencia de infección por E. canis fue de 69.2 %. Ninguna de las variables estudiadas (género, edad, condición corporal, hemorragias relacionadas con plaquetas, trombocitopenia y presencia de garrapatas) mostraron asociación con la infección por E. canis. En conclusión, existe una alta probabilidad que los perros que viven en Yucatán, México, estén infectados con E. canis