IDENTIFICATION of C-3 ACCEPTORS RESPONSIBLE for COMPLEMENT ACTIVATION in CRITHIDIA-FASCICULATA

ESCOLA PAULISTA MED,DISCIPLINA MICOL,RUA BOTUCATU 862,BR-04023 São Paulo,SP,BRAZILESCOLA PAULISTA MED,DISCIPLINA MICOL,RUA BOTUCATU 862,BR-04023 São Paulo,SP,BRAZILWeb of Scienc

Mechanism of resistance to lysis by the alternative complement pathway in Trypanosoma cruzi trypomastigotes: effect of specific monoclonal antibody

ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU 862 80 ANDAR,BR-04023 SAO PAULO,SP,BRAZILESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU 862 80 ANDAR,BR-04023 SAO PAULO,SP,BRAZILWeb of Scienc

Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages

The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host and an insect vector, and adaption of its proteome between lifecycle stages is essential to its survival and virulence. We have optimized a procedure for growing Trypanosoma brucei procyclic form cells in conditions suitable for stable isotope labeling by amino acids in culture (SILAC) and report a comparative proteomic analysis of cultured procyclic form and bloodstream form T. brucei cells. In total we were able to identify 3959 proteins and quantify SILAC ratios for 3553 proteins with a false discovery rate of 0.01. A large number of proteins (10.6%) are differentially regulated by more the 5-fold between lifecycle stages, including those involved in the parasite surface coat, and in mitochondrial and glycosomal energy metabolism. Our proteomic data is broadly in agreement with transcriptomic studies, but with significantly larger fold changes observed at the protein level than at the mRNA level