Ecotoxicology of metal-hydrocarbon mixtures in benthic invertebrates

Metal-hydrocarbon mixtures are becoming increasingly prevalent in natural environments due to expanding industrial activity and urbanization. The ecotoxicology of metal-hydrocarbon mixtures in benthic environments is of particular concern because both classes of contaminants partition to sediments and can thereby exert toxic effects in benthic organisms. Mixtures of dissimilar chemicals (including metals and hydrocarbons) are broadly hypothesized to elicit independent toxic effects however; this hypothesis has little supporting data. The purpose of this dissertation was to test this hypothesis for metal-hydrocarbon mixtures using environmentally relevant exposures and to determine mechanisms for observed interactive effects. Sediment and water-only bioassays were conducted employing the toxic heavy metal cadmium (Cd) and the polynuclear aromatic hydrocarbon phenanthrene (Phen) as model toxicants. Lethal and sublethal effects of singular and combined contaminants were examined in two freshwater species, the epibenthic amphipod Hyalella azteca and the bulk deposit-feeding benthic oligochaete Ilyodrilus templetoni. When interactive toxicity was observed, mixture effects on contaminant bioavailability, bioaccumulation and elimination were tested. As well, mixture effects on bioenergetics parameters were investigated and kinetic modeling was conducted to establish the source of mixture-mediated changes in contaminant bioaccumulation in I. templetoni. Cadmium-Phen mixtures caused independent effects in water-only exposures, but when incorporated into sediments, elicited synergistic lethal effects in H. azteca and antagonistic lethal effects in I. templetoni. Interactive effects were likely caused by Phen-mediated alterations in Cd bioaccumulation that resulted from changes in exposure via feeding. The current basis for assessing ecotoxicological effects of contaminant mixtures in natural environments relies heavily on models derived from dosage-based mixture toxicology with considerably less emphasis on environmental science and biology. Understanding how contaminants interact toxicologically is important, but does not provide all the information necessary for assessing effects in natural populations that encounter contaminant mixtures in a diversity of natural environments. My experiments indicate that exposure source may be more important than dosage-based toxicological interactions in determining contaminant mixture effects in sediment environments. If this trend is widespread, understanding how species are exposed, determining the route of uptake and understanding how environmental characteristics affect exposure may be more important in determining mixture effects than mixture toxicology

Dispersal of meiofauna in a turbulent tidal creek

Traditionally meiofauna have been viewed as strictly infaunal organisms adapted for life between sediment grains. Recent evidence has shown that marine meiofauna also occur in the water column. A set of field experiments investigated processes controlling the abundance of meiofauna in the water column. The transport of meiofauna in a tidal creek was identified to be primarily a passive process resulting from mechanical removal due to current scour. Drifting meiofauna included interstitial, burrowing, and epibenthic species. Copepods, foraminiferans, and juvenile bivalves were suspended in greatest numbers relative to their sediment abundances. Suspension of meiofauna was greatest for species residing in the upper sediment layers and/or occasionally crawling about on the sediment surface. Suspended meiofauna and sediment were well-mixed within the water column, suggesting that behavioral control over water column dispersal was limited once the animals were in the water. Suspension of meiofauna was not a function of winds, time of day, lunar cycle (neap vs. spring tides), or the abundance of meiofauna in the sediment. The abundance of meiofauna in the water was determined primarily by the magnitude of the friction velocity (u.). The emerging concept is that in habitats where water currents are strong enough to lead to sediment scour, meiofauna dispersal is a two-step process with erosion rather than active water column entry (modified by behavior patterns for some species) and subsequent mixing and transport as passive particles

Global Universal Basic Skills: Current Deficits and Implications for World Development

How far is the world away from ensuring that every child obtains the basic skills needed to be internationally competitive? And what would accomplishing this mean for world development? Based on the micro data of international and regional achievement tests, we map achievement onto a common (PISA) scale. We then estimate the share of children not achieving basic skills for 159 countries that cover 98.1% of world population and 99.4% of world GDP. We find that at least two-thirds of the world's youth do not reach basic skill levels, ranging from 24% in North America to 89% in South Asia and 94% in Sub-Saharan Africa. Our economic analysis suggests that the present value of lost world economic output due to missing the goal of global universal basic skills amounts to over $700 trillion over the remaining century, or 11% of discounted GDP

Factors associated with age of first sex among women screened for an observational contraceptive vaginal ring study in Kisumu, Kenya, 2014

Sex at an early age can have later life negative health outcomes. Insights on early sexual activity may help in the development of future health-promotion products and interventions related to pregnancy and sexually transmitted infections (STIs) prevention. Among Kenyan women participating in a contraceptive vaginal ring study, we determined: (1) the proportion with first sex at ≤15 years (early sex) and (2) factors associated with early sex. Baseline data collection and testing for pregnancy, STIs, and HIV were undertaken. A log-binomial or a modified Poisson regression was used to estimate univariable prevalence ratios and multivariable adjusted prevalence ratios (aPRs). Among 332 women aged 18-34 years, the median age of first sex was 16 years, with 40.1% experiencing early sex. Overall, 29.8% reported an older first sex partner (≥5 years); 83.9% indicated lack of readiness or intentions at first sex; and 56.2% received money, gifts, or favors as part of first sex. Early sex was more prevalent among inherited widows (a Luo cultural practice, aPR 1.49), gift recipients (aPR 1.38), women with a forced/unwanted sex history (aPR 1.42), or those with a partner of unknown or positive HIV status in the past 3 months (aPR 1.45). Sex before or at the age 15 may be associated with higher sexual risk behaviors. Complex power and gender relations may additionally influence exposure to sexual risk in our research setting. Les rapports sexuels à un âge précoce peuvent avoir des conséquences négatives sur la santé plus tard dans la vie. Des informations sur l'activité sexuelle précoce peuvent aider au développement de futurs produits et interventions de promotion de la santé liés à la prévention de la grossesse et des infections sexuellement transmissibles (IST). Parmi les femmes kényanes participant à une étude sur l'anneau vaginal contraceptif, nous avons déterminé: (1) la proportion de femmes ayant eu un premier rapport sexuel à ≤15 ans (rapports sexuels précoces) et (2) les facteurs associés aux rapports sexuels précoces. La collecte de données de base et le dépistage de la grossesse, des IST et du VIH ont été entrepris. Une régression log binomiale ou une régression de Poisson modifiée a été utilisée pour estimer les ratios de prévalence univariés et les ratios de prévalence ajustés multivariables (aPR). Chez 332 femmes âgées de 18 à 34 ans, l'âge médian du premier rapport sexuel était de 16 ans, 40,1% ayant eu des rapports sexuels précoces. Dans l'ensemble, 29,8% ont déclaré un premier partenaire sexuel plus âgé (≥ 5 ans); 83,9% ont indiqué un manque de préparation ou d'intentions lors du premier rapport sexuel; et 56,2% ont reçu de l'argent, des cadeaux ou des faveurs dans le cadre de leur premier rapport sexuel. Les rapports sexuels précoces étaient plus fréquents chez les veuves héritées (une pratique culturelle Luo, aPR 1,49), les bénéficiaires de cadeaux (aPR 1,38), les femmes ayant des antécédents sexuels forcés / non désirés (aPR 1,42) ou celles dont le partenaire était séropositif ou inconnu en les 3 derniers mois (aPR 1,45). Les rapports sexuels avant ou à 15 ans peuvent être associés à des comportements sexuels à risque plus élevé. Le pouvoir complexe et les relations de genre peuvent également influencer l'exposition aux risques sexuels dans notre contexte de recherche

A molecule-based 1 : 2 digital demultiplexer

A trichromophoric molecule consisting of a porphyrin linked to both a dihydropyrene and a dihydroindolizine-type photochrome, in combination with a third harmonic generating crystal, functions as a 1:2 digital demultiplexer with photonic inputs and outputs. Each of the two photochromes may be cycled independently between two metastable forms, leading to four photoisomers, three of which are used in the demultiplexer. These isomers interact photochemically with the porphyrin in order to yield the demultiplexer function. With the address input (1064-nm light) turned off, one Output of the device (porphyrin fluorescence) tracks the state of the data input (532-nm light). When the address input is turned on, the second output (absorbance at 572 nm) tracks the state of the data input, while the first output remains off. The demultiplexer does not require chemical or electrical inputs, and can cycle through its operational sequences multiple times

Carotenoporphyrins as selective photodiagnostic agents for tumours.

The covalent binding of a carotene moiety to one phenyl ring and meso-tetraphenyl-substituted porphyrins (see Figure 1) efficiently quenches the photosensitising activity of the porphyrin while a relatively large yield of fluorescence emission around 650 nm is retained. Pharmacokinetic studies performed with two carotenoporphyrins (CPs) and the corresponding porphyrins (Ps) in Balb/c mice bearing an MS-2 fibrosarcoma show that the two Ps give a high selectivity of tumour localisation (tumour/peritumoral tissue ratios of dye concentration ranging between c. 30 and 90 at 24 h after injection of 4.2-8.4 mumol kg-1 in a Cremophor emulsion) and photosensitive tumour necrosis upon red light irradiation. For the same injected doses, the two CPs show no tumour-photosensitising activity even though they localise in the tumour in concentrations of the order of 10-40 micrograms g-1 at 24 h with tumour/peritumoral ratios larger than 10. Thus, the fluorescence emitted by these CPs in the tumour can be used for photodiagnostic purposes with no risk of skin photosensitisation. However, this approach is presently limited by the large accumulation and prolonged retention of the CPs in the liver and spleen

Recognition of envelope and tat protein synthetic peptide analogs by HIV positive sera or plasma

AbstractA series of synthetic peptides corresponding to segments of HIV encoded proteins were selected using criteria described by Welling et al. [(1985) FEBS Lett. 188, 215]. Synthetic peptide analogs to gpl20 (2–13), (55–65), gp41 (582–596) (659–670) and tatIII (71–83) were recognized by 41–67% of sera or plasma from individuals known to be infected with HIV on the basis of virus isolation or Western blot screening. The peptide which reacted with most sera or plasma was gp41 (582–596), a conserved region in the transmembrane glycoprotein. An extended peptide analog, gp41 (579–599), tested against the same samples showed almost 100% reactivity, confirming independent studies identifying a highly immunodominant region of gp41. There was an unexpected high prevalence of antibodies (52%) to the tatIII peptide