Dark complexes of the Calvin-Benson cycle in a physiological perspective

: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK) are two enzymes of the Calvin Benson cycle that stand out for some peculiar properties they have in common: (i) they both use the products of light reactions for catalysis (NADPH for GAPDH, ATP for PRK), (ii) they are both light-regulated through thioredoxins and (iii) they are both involved in the formation of regulatory supramolecular complexes in the dark or low photosynthetic conditions, with or without the regulatory protein CP12. In the complexes, enzymes are transiently inactivated but ready to recover full activity after complex dissociation. Fully active GAPDH and PRK are in large excess for the functioning of the Calvin-Benson cycle, but they can limit the cycle upon complex formation. Complex dissociation contributes to photosynthetic induction. CP12 also controls PRK concentration in model photosynthetic organisms like Arabidopsis thaliana and Chlamydomonas reinhardtii. The review combines in vivo and in vitro data into an integrated physiological view of the role of GAPDH and PRK dark complexes in the regulation of photosynthesis

Bundles over Nearly-Kahler Homogeneous Spaces in Heterotic String Theory

We construct heterotic vacua based on six-dimensional nearly-Kahler homogeneous manifolds and non-trivial vector bundles thereon. Our examples are based on three specific group coset spaces. It is shown how to construct line bundles over these spaces, compute their properties and build up vector bundles consistent with supersymmetry and anomaly cancelation. It turns out that the most interesting coset is $SU(3)/U(1)^2$. This space supports a large number of vector bundles which lead to consistent heterotic vacua, some of them with three chiral families.Comment: 32 pages, reference adde

Heterotic String Compactifications on Half-flat Manifolds II

In this paper, we continue the analysis of heterotic string compactifications on half-flat mirror manifolds by including the 10-dimensional gauge fields. It is argued, that the heterotic Bianchi identity is solved by a variant of the standard embedding. Then, the resulting gauge group in four dimensions is still E6 despite the fact that the Levi-Civita connection has SO(6) holonomy. We derive the associated four-dimensional effective theories including matter field terms for such compactifications. The results are also extended to more general manifolds with SU(3) structure.Comment: 31 page

Moduli Stabilisation in Heterotic Models with Standard Embedding

In this note we analyse the issue of moduli stabilisation in 4d models obtained from heterotic string compactifications on manifolds with SU(3) structure with standard embedding. In order to deal with tractable models we first integrate out the massive fields. We argue that one can not only integrate out the moduli fields, but along the way one has to truncate also the corresponding matter fields. We show that the effective models obtained in this way do not have satisfactory solutions. We also look for stabilised vacua which take into account the presence of the matter fields. We argue that this also fails due to a no-go theorem for Minkowski vacua in the moduli sector which we prove in the end. The main ingredient for this no-go theorem is the constraint on the fluxes which comes from the Bianchi identity.Comment: 20 pages, LaTeX; references adde

La cultura locale di Roma e delle sue periferie urbane nella rappresentazione dei resti archeologici - The local culture of Rome and its urban suburbs in the representation of the archaeological remains

Nel presente lavoro viene proposta l’analisi della relazione come luogo del problema che caratterizza l’intervento psicologico clinico quale alternativa alla diagnosi. Tre esempi clinici aiutano a cogliere come la relazione sia il luogo del problema che s’intende affrontare; sia con il lavoro d’assistenza in ambito familiare, che scolastico o sociale. La funzione psicologica può cambiare radicalmente il lavoro assistenziale, mutando obiettivi inutili e obsoleti in interventi efficaci. Ciò è possibile se l’intervento cambia il proprio obiettivo, spostandolo dalla singola persona disabile o problematica, alla relazione entro la quale l’intervento si contestualizza.The research, carried out within a project research of national interest (PRIN) directed by archaeologist Professor Clementina Panella, has the purpose to detect the local culture of inhabitants of the center and the outskirts of Rome, paying attention to the archaeological remains and their cultural contribution. Three components of local culture emerged in this research; all of them are reported to a cultural context that people feel as organized by the pursuit of power and the enhancement of the mass media as factors that give notoriety and popularity then power. One of the three cultural repertoires, characterized by young participants and residents in the outskirts of Rome, enhances the archaeological remains as components of a fun culture. The fun, in combination with culture, is experienced as an effective alternative to the power and the pursuit of powe

Comparing Radiotherapy Patient Flows in England and Italy

Research into radiotherapy patient flows in England and Italy investigated the degree of self-sufficiency in related service areas across each country. The respective results showed very different patterns, with there apparently being greater patient flow and less self-sufficiency for services in Italy, compared to England. However, close inspection suggested the results were not strictly comparable, despite the data, methodology and analyses being similar and entirely fitting in both cases. The respective studies were consequently compared for a range of criteria: how radiotherapy services are commissioned and provided; cultural expectations; base area definitions; data used; analyses undertaken; and research results. It was found that direct comparisons could not necessarily be drawn because cultural, conceptual, organisational and systemic factors in the delivery of services within each country influenced the results. Therefore, such factors should be taken into account when comparing health services in different countries, particularly in respect of patient flows and service self-sufficiency

Comparison of presepsin, procalcitonin, interleukin-8 and C-reactive protein in predicting bacteraemia in febrile neutropenic adult patients with haematological malignancies

Bacterial infections represent life-threatening complications in patients with febrile neutropenia (FN). Diagnostic biomarkers of infections may help to differentiate bacteraemia from non-bacteraemia FN. We aimed to evaluate the utility of procalcitonin (PCT), presepsin (PS), C-reactive protein (CRP) and interleukin-8 (IL-8) as biomarkers of bacteraemia in adult FN patients with haematological malignancies. Concentrations of PCT, PS, CRP and IL-8 were prospectively measured in 36 FN episodes experienced by 28 oncohaematological patients. 11 out of 36 episodes were classified as bacteraemia. PCT was the best biomarker to predict bacteraemia with the area under the curve (AUC) ROC of 0,9; specificity 100% and positive predictive value 100%, while the most sensitive was IL-8 (90,9%) with AUC ROC of 0,88 and negative predictive value 95,2%. All patients with PCT concentrations above 1,6 \u3bcg/l had bacteraemia. Patients with IL-8 concentrations superior to 170 pg/ml had a 40 times higher risk for bacteraemia than the ones with lower levels. Patients with PS concentrations superior to 410 pg/ml had 24 times higher risk for bacteraemia than the patients with lower levels. PCT has higher accuracy than CRP, IL-8 and PS in predicting bacteraemia in adult hematologic patients with FN

Conformational Disorder Analysis of the Conditionally Disordered Protein CP12 from Arabidopsis thaliana in Its Different Redox States

CP12 is a redox-dependent conditionally disordered protein universally distributed in oxygenic photosynthetic organisms. It is primarily known as a light-dependent redox switch regulating the reductive step of the metabolic phase of photosynthesis. In the present study, a small angle X-ray scattering (SAXS) analysis of recombinant Arabidopsis CP12 (AtCP12) in a reduced and oxidized form confirmed the highly disordered nature of this regulatory protein. However, it clearly pointed out a decrease in the average size and a lower level of conformational disorder upon oxidation. We compared the experimental data with the theoretical profiles of pools of conformers generated with different assumptions and show that the reduced form is fully disordered, whereas the oxidized form is better described by conformers comprising both the circular motif around the C-terminal disulfide bond detected in previous structural analysis and the N-terminal disulfide bond. Despite the fact that disulfide bridges are usually thought to confer rigidity to protein structures, in the oxidized AtCP12, their presence coexists with a disordered nature. Our results rule out the existence of significant amounts of structured and compact conformations of free AtCP12 in a solution, even in its oxidized form, thereby highlighting the importance of recruiting partner proteins to complete its structured final folding

The Thioredoxin-Regulated α-Amylase 3 of Arabidopsis thaliana Is a Target of S-Glutathionylation

Reactive oxygen species (ROS) are produced in cells as normal cellular metabolic by-products. ROS concentration is normally low, but it increases under stress conditions. To stand ROS exposure, organisms evolved series of responsive mechanisms. One such mechanism is protein S-glutathionylation. S-glutathionylation is a post-translational modification typically occurring in response to oxidative stress, in which a glutathione reacts with cysteinyl residues, protecting them from overoxidation. α-Amylases are glucan hydrolases that cleave α-1,4-glucosidic bonds in starch. The Arabidopsis genome contains three genes encoding α-amylases. The sole chloroplastic member, AtAMY3, is involved in osmotic stress response and stomatal opening and is redox-regulated by thioredoxins. Here we show that AtAMY3 activity was sensitive to ROS, such as H2O2. Treatments with H2O2 inhibited enzyme activity and part of the inhibition was irreversible. However, in the presence of glutathione this irreversible inhibition was prevented through S-glutathionylation. The activity of oxidized AtAMY3 was completely restored by simultaneous reduction by both glutaredoxin (specific for the removal of glutathione-mixed disulfide) and thioredoxin (specific for the reduction of protein disulfide), supporting a possible liaison between both redox modifications. By comparing free cysteine residues between reduced and GSSG-treated AtAMY3 and performing oxidation experiments of Cys-to-Ser variants of AtAMY3 using biotin-conjugated GSSG, we could demonstrate that at least three distinct cysteinyl residues can be oxidized/glutathionylated, among those the two previously identified catalytic cysteines, Cys499 and Cys587. Measuring the pKa values of the catalytic cysteines by alkylation at different pHs and enzyme activity measurement (pKa1 = 5.70 ± 0.28; pKa2 = 7.83 ± 0.12) showed the tendency of one of the two catalytic cysteines to deprotonation, even at physiological pHs, supporting its propensity to undergo redox post-translational modifications. Taking into account previous and present findings, a functional model for redox regulation of AtAMY3 is proposed