Comparative transcriptome analysis of genes involved in paradormant bud release response in ‘Summer Black’ grape

Grapevines possess a hierarchy of buds, and the fruitful winter bud forms the foundation of the two-crop-a-year cultivation system, yielding biannual harvests. Throughout its developmental stages, the winter bud sequentially undergoes paradormancy, endodormancy, and ecodormancy to ensure survival in challenging environmental conditions. Releasing the endodormancy of winter bud results in the first crop yield, while breaking the paradormancy of winter bud allows for the second crop harvest. Hydrogen cyanamide serves as an agent to break endodormancy, which counteracting the inhibitory effects of ABA, while H2O2 and ethylene function as signaling molecules in the process of endodormancy release. In the context of breaking paradormancy, common agronomic practices include short pruning and hydrogen cyanamide treatment. However, the mechanism of hydrogen cyanamide contributes to this process remains unknown. This study confirms that hydrogen cyanamide treatment significantly improved both the speed and uniformity of bud sprouting, while short pruning proved to be an effective method for releasing paradormancy until August. This observation highlights the role of apical dominance as a primary inhibitory factor in suppressing the sprouting of paradormant winter bud. Comparative transcriptome analysis revealed that the sixth node winter bud convert to apical tissue following short pruning and established a polar auxin transport canal through the upregulated expression of VvPIN3 and VvTIR1. Moreover, short pruning induced the generation of reactive oxygen species, and wounding, ethylene, and H2O2 collectively acted as stimulating signals and amplified effects through the MAPK cascade. In contrast, hydrogen cyanamide treatment directly disrupted mitochondrial function, resulting in ROS production and an extended efficacy of the growth hormone signaling pathway induction

Integrated Transcriptome and Metabolome Analysis Revealed the Causal Agent of Primary Bud Necrosis in ‘Summer Black’ Grape

Primary bud necrosis of grape buds is a physiological disorder that leads to decreased berry yield and has a catastrophic impact on the double cropping system in sub-tropical areas. The pathogenic mechanisms and potential solutions remain unknown. In this study, the progression and irreversibility patterns of primary bud necrosis in ‘Summer Black’ were examined via staining and transmission electron microscopy observation. Primary bud necrosis was initiated at 60 days after bud break and was characterized by plasmolysis, mitochondrial swelling, and severe damage to other organelles. To reveal the underlying regulatory networks, winter buds were collected during primary bud necrosis progression for integrated transcriptome and metabolome analysis. The accumulation of reactive oxygen species and subsequent signaling cascades disrupted the regulation systems for cellular protein quality. ROS cascade reactions were related to mitochondrial stress that can lead to mitochondrial dysfunction, lipid peroxidation causing damage to membrane structure, and endoplasmic reticulum stress leading to misfolded protein aggregates. All these factors ultimately resulted in primary bud necrosis. Visible tissue browning was associated with the oxidation and decreased levels of flavonoids during primary bud necrosis, while the products of polyunsaturated fatty acids and stilbenes exhibited an increasing trend, leading to a shift in carbon flow from flavonoids to stilbene. Increased ethylene may be closely related to primary bud necrosis, while auxin accelerated cell growth and alleviated necrosis by co-chaperone VvP23-regulated redistribution of auxin in meristem cells. Altogether, this study provides important clues for further study on primary bud necrosis

A Review of Super-High-Temperature Heat Pumps over 100 °C

The high-temperature heat pump, as a low-carbonization technology, has broad application prospects in replacing boiler heating, reducing carbon dioxide emissions, and improving the energy utilization efficiency. In this paper, the working fluid, cycle process, key equipment (compressor), and application scenarios of high-temperature heat pumps are introduced in detail. Firstly, the research direction of the working fluid is introduced and the existing working fluid substitution is analyzed and summarized. Then, the characteristics of different heat pump cycles such as compression, absorption, and hybrid heat pumps are introduced. In the aspect of key equipment, the application range and research status of different types of compressors are emphatically introduced. Finally, the application scenario of high-temperature heat pumps is prospected. In addition to the application of industrial heating, it is often used for heat storage to improve the regulatory characteristics of the system. The new heat pump electricity storage system has great application potential in the field of renewable energy consumption. Based on the above analysis of high-temperature heat pumps, four development prospects are put forward: low-Global-Warming-Potential (GWP) working fluid; cycles of temperature lift greater than 80 °C; a compressor with better high-temperature performance; and circulation characteristics of heat pump electricity storage

HGMAN: Multi-Hop and Multi-Answer Question Answering Based on Heterogeneous Knowledge Graph (Student Abstract)

Multi-hop question answering models based on knowledge graph have been extensively studied. Most existing models predict a single answer with the highest probability by ranking candidate answers. However, they are stuck in predicting all the right answers caused by the ranking method. In this paper, we propose a novel model that converts the ranking of candidate answers into individual predictions for each candidate, named heterogeneous knowledge graph based multi-hop and multi-answer model (HGMAN). HGMAN is capable of capturing more informative representations for relations assisted by our heterogeneous graph, which consists of multiple entity nodes and relation nodes. We rely on graph convolutional network for multi-hop reasoning and then binary classification for each node to get multiple answers. Experimental results on MetaQA dataset show the performance of our proposed model over all baselines

Biochar application significantly affects the N pool and microbial community structure in purple and paddy soils

Background The increasing demand for food production has resulted in the use of large quantities of chemical fertilizers. This has created major environmental problems, such as increased ammonia volatilization, N2O emission, and nitrogen (N) leaching from agricultural soil. In particular, the utilization rate of N fertilizer is low in subtropical southern parts of China due to high rainfall. This causes not only large financial losses in agriculture, but also serious environmental pollution. Methods In this study, 16S rDNA-based analysis and static-chamber gas chromatography were used to elucidate the effects of continuous straw biochar application on the N pool and bacteria environment in two typical soil types, purple and paddy soils, in southern China. Results Straw biochar application (1) improved the soil N pool in both rhizosphere and non-rhizosphere soils; (2) significantly reduced the emission of N2O, with no difference in emission between 1 and 2 years of application; (3) increased the abundance of N-processing bacteria in the soil and altered the bacterial community structure; and (4) improved the tobacco yield and N use efficiency in paddy soil. These findings suggest that, in southern China, the application of straw biochar can promote N transformation in purple and paddy soils and reduce the emission of the greenhouse gas N2O

Research and Application of Biochar in Soil CO2 Emission, Fertility, and Microorganisms: A Sustainable Solution to Solve China’s Agricultural Straw Burning Problem

This study aimed to explore a new way to address the burning of agricultural waste in China while achieving the sustainable use of it. Three agricultural wastes (Wheat straw, peanut shell, and rice husk) were slowly pyrolyzed into biochar, which was subsequently added to the soil to reduce CO2 emissions from the soil, and to improve soil fertility as well as microbial community structure. The biochar and raw materials were added to the soil and cultured under controlled conditions, and then the CO2 emissions produced from the mixing. At the same time, this study used pot experiments to determine the effects of biochar on tobacco soil physical and chemical properties and, therefore, the microbial communities of the soil. This study suggests that (1) biochar can effectively reduce soil CO2 emission rate. Compared with the control, peanut shell biochar could reduce the total CO2 emissions of soil by 33.41%, and the total CO2 emissions of wheat straw biochar treatment was 90.25% lower than that of wheat straw treatment. (2) The soil’s physical and chemical properties were improved. The soil bulk density of wheat straw biochar treatment kept 34.57% lower than that of the control as well as 21.15% lower than that of wheat straw treatment. The soil’s organic carbon of peanut shell biochar treatment was 87.62% more than that of peanut shell treatment. (3) Biochar changed soil microbial community structure. (4) Biochar is suitable for tobacco growth. Peanut husk biochar significantly increased the total biomass of tobacco, and wheat straw biochar significantly increased tobacco root vigor. This study concluded that processing Chinese agricultural waste into biochar and adding it to the soil instead of burning it directly would be an effective means to reduce greenhouse gas emissions, to improve soil, and to promote crop growth

Image_2_Comparative transcriptome analysis of genes involved in paradormant bud release response in ‘Summer Black’ grape.jpeg

Grapevines possess a hierarchy of buds, and the fruitful winter bud forms the foundation of the two-crop-a-year cultivation system, yielding biannual harvests. Throughout its developmental stages, the winter bud sequentially undergoes paradormancy, endodormancy, and ecodormancy to ensure survival in challenging environmental conditions. Releasing the endodormancy of winter bud results in the first crop yield, while breaking the paradormancy of winter bud allows for the second crop harvest. Hydrogen cyanamide serves as an agent to break endodormancy, which counteracting the inhibitory effects of ABA, while H2O2 and ethylene function as signaling molecules in the process of endodormancy release. In the context of breaking paradormancy, common agronomic practices include short pruning and hydrogen cyanamide treatment. However, the mechanism of hydrogen cyanamide contributes to this process remains unknown. This study confirms that hydrogen cyanamide treatment significantly improved both the speed and uniformity of bud sprouting, while short pruning proved to be an effective method for releasing paradormancy until August. This observation highlights the role of apical dominance as a primary inhibitory factor in suppressing the sprouting of paradormant winter bud. Comparative transcriptome analysis revealed that the sixth node winter bud convert to apical tissue following short pruning and established a polar auxin transport canal through the upregulated expression of VvPIN3 and VvTIR1. Moreover, short pruning induced the generation of reactive oxygen species, and wounding, ethylene, and H2O2 collectively acted as stimulating signals and amplified effects through the MAPK cascade. In contrast, hydrogen cyanamide treatment directly disrupted mitochondrial function, resulting in ROS production and an extended efficacy of the growth hormone signaling pathway induction.</p

Image_3_Comparative transcriptome analysis of genes involved in paradormant bud release response in ‘Summer Black’ grape.jpeg

Grapevines possess a hierarchy of buds, and the fruitful winter bud forms the foundation of the two-crop-a-year cultivation system, yielding biannual harvests. Throughout its developmental stages, the winter bud sequentially undergoes paradormancy, endodormancy, and ecodormancy to ensure survival in challenging environmental conditions. Releasing the endodormancy of winter bud results in the first crop yield, while breaking the paradormancy of winter bud allows for the second crop harvest. Hydrogen cyanamide serves as an agent to break endodormancy, which counteracting the inhibitory effects of ABA, while H2O2 and ethylene function as signaling molecules in the process of endodormancy release. In the context of breaking paradormancy, common agronomic practices include short pruning and hydrogen cyanamide treatment. However, the mechanism of hydrogen cyanamide contributes to this process remains unknown. This study confirms that hydrogen cyanamide treatment significantly improved both the speed and uniformity of bud sprouting, while short pruning proved to be an effective method for releasing paradormancy until August. This observation highlights the role of apical dominance as a primary inhibitory factor in suppressing the sprouting of paradormant winter bud. Comparative transcriptome analysis revealed that the sixth node winter bud convert to apical tissue following short pruning and established a polar auxin transport canal through the upregulated expression of VvPIN3 and VvTIR1. Moreover, short pruning induced the generation of reactive oxygen species, and wounding, ethylene, and H2O2 collectively acted as stimulating signals and amplified effects through the MAPK cascade. In contrast, hydrogen cyanamide treatment directly disrupted mitochondrial function, resulting in ROS production and an extended efficacy of the growth hormone signaling pathway induction.</p