Coating titania nanoparticles with epoxy-containing catechol polymers via Cu(0)-living radical polymerization as intelligent enzyme carriers

Immobilization of enzyme could offer the biocatalyst with increased stability and important recoverability, which plays a vital role in the enzyme’s industrial applications. In this study, we present a new strategy to build an intelligent enzyme carrier by coating titania nanoparticles with thermoresponsive epoxy-functionalized polymers. Zero-valent copper-mediated living radical polymerization (Cu(0)-LRP) was utilized herein to copolymerize N-isopropylacrylamide (NIPAM) and glycidyl acrylate (GA) directly from an unprotected dopamine-functionalized initiator to obtain an epoxy-containing polymer with terminal anchor for the “grafting to” or “one-pot” modification of titania nanoparticles. A rhodamine B-labeled laccase has been subsequently used as a model enzyme for successful immobilization to yield an intelligent titania/laccase hybrid bifunctional catalyst. The immobilized laccase has shown excellent thermal stability under ambient or even relatively high temperature above the lower critical solution temperature (LCST) at which temperature the hybrid particles could be facilely recovered for reuse. The enzyme activity could be maintained during the repeated use after recovery and enzymatic degradation of bisphenol A was proven to be efficient. The photocatalytic ability of titania was also investigated by fast degradation of rhodamine B under the excitation of simulated sunlight. Therefore, this study has provided a facile strategy for the immobilization of metal oxide catalysts with enzymes, which constructs a novel bifunctional catalyst that will be promising for the “one-pot” degradation of different organic pollutants

Structural and biochemical studies of human lysine methyltransferase Smyd3 reveal the important functional roles of its post-SET and TPR domains and the regulation of its activity by DNA binding

The SET- and MYND-domain containing (Smyd) proteins constitute a special subfamily of the SET-containing lysine methyltransferases. Here we present the structure of full-length human Smyd3 in complex with S-adenosyl-l-homocysteine at 2.8 Å resolution. Smyd3 affords the first example that other region(s) besides the SET domain and its flanking regions participate in the formation of the active site. Structural analysis shows that the previously uncharacterized C-terminal domain of Smyd3 contains a tetratrico-peptide repeat (TPR) domain which together with the SET and post-SET domains forms a deep, narrow substrate binding pocket. Our data demonstrate the important roles of both TPR and post-SET domains in the histone lysine methyltransferase (HKMT) activity of Smyd3, and show that the hydroxyl group of Tyr239 is critical for the enzymatic activity. The characteristic MYND domain is located nearby to the substrate binding pocket and exhibits a largely positively charged surface. Further biochemical assays show that DNA binding of Smyd3 can stimulate its HKMT activity and the process may be mediated via the MYND domain through direct DNA binding

CCL4 participates in the reprogramming of glucose metabolism induced by ALV-J infection in chicken macrophages

Interferon and chemokine-mediated immune responses are two general antiviral programs of the innate immune system in response to viral infections and have recently emerged as important players in systemic metabolism. This study found that the chemokine CCL4 is negatively regulated by glucose metabolism and avian leukosis virus subgroup J (ALV-J) infection in chicken macrophages. Low expression levels of CCL4 define this immune response to high glucose treatment or ALV-J infection. Moreover, the ALV-J envelope protein is responsible for CCL4 inhibition. We confirmed that CCL4 could inhibit glucose metabolism and ALV-J replication in chicken macrophages. The present study provides novel insights into the antiviral defense mechanism and metabolic regulation of the chemokine CCL4 in chicken macrophages

Interactive Cognitive Motor Training: A Promising Approach for Sustainable Improvement of Balance in Older Adults

Physical exercise has been shown to improve balance, gait, and cognitive function in older adults. Interactive cognitive-motor training (ICMT) combines physical exercise and cognitive stimulation, but few studies have focused on the effect of ICMT on static and dynamic balance in older adults. This study aims to improve the balance of older adults to reduce fall-related injuries for sustainable development goals. We randomly assigned 38 older adults to either the ICMT group (n = 22) or the control group (n = 16). The ICMT group participated in 60-min exercise sessions three times a week for 12 weeks, while the control group maintained their regular activities. The static and dynamic balances were assessed before and after the intervention. The results indicated the ICMT group demonstrated significant improvements in static balance, specifically in swing path and velocity (V) in the medial-lateral (M-L) direction with eyes open (p p p < 0.05). In conclusion, ICMT effectively enhances static balance and maintains dynamic balance in older adults

A systematic review on the current research of digital twin in automotive application

Digital Twin (DT) has been considered one of the most promising technologies promoting the development of the industry and has successfully achieved significant applications in many fields including national defense, intelligent manufacturing, smart cities, and so on. In addition, DT is gradually used in the research of automobiles which are necessary means of transportation in people's life. A systematic review of the definitions of DT and its applications in automotive-related fields reported in the literature is presented in this paper, with the overall goal to summarize almost all the related research achievements and stimulate innovative thinking. The digital twin, its structure, and its evolution are systematically reviewed. And all reviewed automotive applications of DT are divided into three categories: automotive industry, transportation, and batteries which is the research hotspot in the automotive field. Besides, these methods and ideas will be emphasized in this paper. Particularly, future development directions of digital twins in the automotive field are highlighted

Modulation of ROS in Nanosecond-Pulsed Plasma-Activated Media for Dosage-Dependent Cancer Cell Inactivation \u3ci\u3ein vitro\u3c/i\u3e

Dosage control of reactive oxygen and nitrogen species (RONS) is critical to low-temperature plasma applications in cancer therapy. Production of RONS by atmospheric pressure, nonequilibrium plasmas in contact with liquid may be modulated via plasma conditions including plasma treatment time and pulse voltage and repetition frequency. In this study, a terephthalic acid-based probe was used to measure hydroxyl radicals [OHaq] in water exposed to plasma and to demonstrate that the OHag concentration increases linearly with treatment time. Fluorometric measurements of hydrogen peroxide concentration in plasma-activated water show a linear relationship between the H2O2 production rate and the pulse repetition frequency of the plasma. In vitro plasma treatment of cancer cells shows that pancreatic (Pan02) and breast (4T1-Luc) cancer cells have different sensitivities to plasma exposure. The dependence of Pan02 cell viability on plasma treatment time or pulse voltage is nonlinear. The system described here for generation and delivery of reactive oxygen species from a nanosecond pulsed plasma jet represents a promising alternative approach to cancer therapy

Prediction of Surface Roughness in Gas-Solid Two-Phase Abrasive Flow Machining Based on Multivariate Linear Equation

The main purpose of this study is to explore a surface roughness prediction model of Gas-Solid Two-Phase Abrasive Flow Machining. In order to achieve the above purpose, an orthogonal experiment was carried out. Q235 steel as processing material and white corundum with different particle sizes as abrasive particles were used in the experiment. Shape and spindle speed were the main reference factors. The range method and factor trend graph are used to comprehensively analyze the experimental results of different processing stages of the detection point, and the optimal parameter combination of A3B2C1D2 was obtained. According to the experimental results, a multiple linear regression equation was established to predict the surface roughness, and the experimental results were solved and significantly analyzed by software to obtain a highly reliable prediction model. Through experiments, modeling and verification, it is known that the maximum error between the obtained model and the actual value is 0.339 μm and the average error is 0.00844 μm, which can better predict the surface roughness of the gas-solid two-phase flow abrasive pool

The genesis model of carbonate cementation in the tight oil reservoir: A case of Chang 6 oil layers of the Upper Triassic Yanchang Formation in the western Jiyuan area, Ordos Basin, China

Carbonate cementation is one of the significant tightness factors in Chang 6 reservoir of the western Jiyuan (WJY) area. Based on the observation of core and thin sections, connecting-well profile analysis as well as carbon and oxygen isotope analysis, it is found that ferrocalcite is the main carbonate cements in the Chang 6 reservoir of the WJY area. The single sand body controls the development of carbonate cements macroscopically. Both carbonate cements and calcite veins hold similar diagenetic conditions: the dissolution of plagioclase is the main calcium source and the de-acidification of organic acids is the main carbon source. The diagenetic stage is identified as the mesogenetic A stage. The sedimentary environment is of low salinity. Accordingly, the development model of carbonate cementation in Chang 6 reservoir is summarized into three types: “eggshell pattern,” “cutting pattern,” and “favorable reservoir pattern.” The development degree of carbonate cementation affects the physical properties of reservoir

PI3K/Akt-independent negative regulation of JNK signaling by MKP-7 after cerebral ischemia in rat hippocampus

Abstract Background The inactivation of c-Jun N-terminal kinase (JNK) is associated with anti-apoptotic and anti-inflammatory effects in cerebral ischemia, which can be induced by an imbalance between upstream phosphatases and kinases. Result Mitogen-activated protein kinase phosphatase 7 (MKP-7) was upregulated significantly at 4 h of reperfusion postischemia in rat hippocampi. By administration of cycloheximide or siRNA against mitogen-activated protein kinase phosphatase 7 (MKP-7) in a rat model of ischemia/reperfusion, an obvious enhancement of JNK activity was observed in 4 h of reperfusion following ischemia, suggesting MKP-7 was involved in JNK inactivation after ischemia. The subcellular localization of MKP-7 altered after ischemia, and the inhibition of MKP-7 nuclear export by Leptomycin B up-regulated JNK activity. Although PI3K/Akt inhibition could block downregulation of JNK activity through SEK1 and MKK-7 activation, PI3K/Akt activity was not associated with the regulation of JNK by MKP-7. Conclusions MKP-7, independently of PI3K/Akt pathway, played a key role in downregulation of JNK activity after ischemia in the rat hippocampus, and the export of MKP-7 from the nucleus was involved in downregulation of cytoplasmic JNK activity in response to ischemic stimuli.</p