Transcriptome Sequencing and Comparative Analysis of Saccharina japonica (Laminariales, Phaeophyceae) under Blue Light Induction

BACKGROUND: Light has significant effect on the growth and development of Saccharina japonica, but there are limited reports on blue light mediated physiological responses and molecular mechanism. In this study, high-throughput paired-end RNA-sequencing (RNA-Seq) technology was applied to transcriptomes of S. japonica exposed to blue light and darkness, respectively. Comparative analysis of gene expression was designed to correlate the effect of blue light and physiological mechanisms on the molecular level. PRINCIPAL FINDINGS: RNA-seq analysis yielded 70,497 non-redundant unigenes with an average length of 538 bp. 28,358 (40.2%) functional transcripts encoding regions were identified. Annotation through Swissprot, Nr, GO, KEGG, and COG databases showed 25,924 unigenes compared well (E-value <10(-5)) with known gene sequences, and 43 unigenes were putative BL photoreceptor. 10,440 unigenes were classified into Gene Ontology, and 8,476 unigenes were involved in 114 known pathways. Based on RPKM values, 11,660 (16.5%) differentially expressed unigenes were detected between blue light and dark exposed treatments, including 7,808 upregulated and 3,852 downregulated unigenes, suggesting S. japonica had undergone extensive transcriptome re-orchestration during BL exposure. The BL-specific responsive genes were indentified to function in processes of circadian rhythm, flavonoid biosynthesis, photoreactivation and photomorphogenesis. SIGNIFICANCE: Transcriptome profiling of S. japonica provides clues to potential genes identification and future functional genomics study. The global survey of expression changes under blue light will enhance our understanding of molecular mechanisms underlying blue light induced responses in lower plants as well as facilitate future blue light photoreceptor identification and specific responsive pathways analysis

Prophet: Conflict-Free Sharding Blockchain via Byzantine-Tolerant Deterministic Ordering

Sharding scales throughput by splitting blockchain nodes into parallel groups. However, different shards' independent and random scheduling for cross-shard transactions results in numerous conflicts and aborts, since cross-shard transactions from different shards may access the same account. A deterministic ordering can eliminate conflicts by determining a global order for transactions before processing, as proved in the database field. Unfortunately, due to the intertwining of the Byzantine environment and information isolation among shards, there is no trusted party able to predetermine such an order for cross-shard transactions. To tackle this challenge, this paper proposes Prophet, a conflict-free sharding blockchain based on Byzantine-tolerant deterministic ordering. It first depends on untrusted self-organizing coalitions of nodes from different shards to pre-execute cross-shard transactions for prerequisite information about ordering. It then determines a trusted global order based on stateless ordering and post-verification for pre-executed results, through shard cooperation. Following the order, the shards thus orderly execute and commit transactions without conflicts. Prophet orchestrates the pre-execution, ordering, and execution processes in the sharding consensus for minimal overhead. We rigorously prove the determinism and serializability of transactions under the Byzantine and sharded environment. An evaluation of our prototype shows that Prophet improves the throughput by $3.11\times$ and achieves nearly no aborts on 1 million Ethereum transactions compared with state-of-the-art sharding