Linking Loneliness and Use of Social Media

Currently, Internet-based social media have an increased influence on society, and the use of social media has penetrated into every aspect of life. Because of online use of social media, individuals are connected through screen virtually but isolated from each other physically. Previous conventional offline social life is decreasing and a new psychological problem arises: loneliness. Empirical studies found that despite the fact that this generation has more devices and technologies that help people to stay connected, the feeling of loneliness in the 21st century is the highest of all times so far. This thesis will combine past empirical studies of the relevant field with the theory of use and gratification, aiming to identify the relationship between use of social media and loneliness. This exploratory study was carried out in the University of Helsinki through the online survey, Facebook was chosen as an example of social media that is widely used among university students, and 112 valid survey samples were collected. Through quantitative research method, four major findings are found: (1) Loneliness is positively correlated with Facebook motivation, which can be interpreted as loneliness motivates people to use Facebook; (2) Loneliness is negatively correlated with the number of Facebook friends, which indicates that a larger social network size can combat loneliness; (3) Females use Facebook more intensively than males than people who prefer not to say their gender; (4) Asian/Pacific islander ethnicity group shows the highest motivation to use Facebook, followed by white ethnicity and then others

Pharmacological Study of Phenolic Components in Parkinson's Disease

In this study, cell experiments were conducted to investigate the effects of extracts on cell viability and apoptosis of Parkinson model in vitro, as well as the expression of cysteine protease-3 (Caspase-3) and B lymphocytoma-2-associated X protein (BAX). The results showed that extract of phenols could improve the loss of cell viability and apoptosis induced by MPP+, and inhibit the enhanced expression of Bax and Caspase-3 by MPP+. The potential targets and signaling pathways of phenols in the treatment of Parkinson's disease were predicted by network pharmacology

The role of JAM-B in cancer and cancer metastasis (Review)

The junctional adhesion molecule B (JAM-B) is a multifunctional transmembrane protein, which belongs to the immunoglobulin superfamily (IgSF). JAM-B is localized to cell-cell contacts and enriched at cell junctions in epithelial and endothelial cells, as well as on the surface of erythrocytes, leukocytes, and platelets. Recent research in this field has shown that JAM-B plays an important role in numerous cellular processes, such as tight junction assembly, spermatogenesis, regulation of paracellular permeability, leukocytic transmigration, angiogenesis, tumor metastasis and cell proliferation. This study provides a new research direction for the diagnosis and treatment of relevant diseases. In this review, we briefly focus on what is currently known about the structure, function, and mechanism of JAM-B, with particular emphasis on cancer

Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer

The junctional adhesion molecule (JAMs) family belongs to the immunoglobulin subfamily involved in the formation of tight junctions (TJ) in both endothelial and epithelial cells. Aberrant expression of JAM-2 is associated with cancer progression but little work has been carried out in discovering how this affects changes in cell behaviour. The present study aimed to examine the expression of JAM-2 in human colon cancer specimens and cell lines and its role in the development of colon cancer. JAM-2 expression in human colon cancer specimens (normal, n=75; cancer, n=94) and cell lines was analysed using quantitative real-time PCR and conventional RT-PCR. Colon cancer cells were stably transfected with a mammalian expression vector to overexpress JAM-2-Flag. The effect on growth, adhesion and migration following overexpression of JAM-2 was then investigated using in vitro models. TJ function was assessed using a trans-epithelial resistance assay (TER, with an EVOM voltammeter). JAM-2 was lowly expressed in colon cancer cells such as RKO, HT115. JAM-2 overexpression in RKO cells (RKO-JAM-2) and HT115 cells (HT115-JAM-2) showed retarded adhesion (P<0.05). An in vivo tumour model showed that RKO-JAM-2 had significantly reduced growth (P<0.05), invasion (P<0.05) and migration (P<0.05) as well as in HT115-JAM-2, except on proliferation and migration. Expression of JAM-2 resulted in a significant increase in TER and decrease in permeability of polarized monolayers (P<0.05). Further analysis of JAM-2 transcript levels against clinical aspects demonstrated that the decreasing JAM-2 expression correlated to disease progression, metastasis and poor survival. Taken together, JAM-2 may function as a putative tumour suppressor in the progression and metastasis of colorectal cance

Involvement of C4 Protein of Beet Severe Curly Top Virus (Family Geminiviridae) in Virus Movement

Background: Beet severe curly top virus (BSCTV) is a leafhopper transmitted geminivirus with a monopartite genome. C4 proteins encoded by geminivirus play an important role in virus/plant interaction. Methods and Findings: To understand the function of C4 encoded by BSCTV, two BSCTV mutants were constructed by introducing termination codons in ORF C4 without affecting the amino acids encoded by overlapping ORF Rep. BSCTV mutants containing disrupted ORF C4 retained the ability to replicate in Arabidopsis protoplasts and in the agro-inoculated leaf discs of N. benthamiana, suggesting C4 is not required for virus DNA replication. However, both mutants did not accumulate viral DNA in newly emerged leaves of inoculated N. benthamiana and Arabidopsis, and the inoculated plants were asymptomatic. We also showed that C4 expression in plant could help C4 deficient BSCTV mutants to move systemically. C4 was localized in the cytosol and the nucleus in both Arabidopsis protoplasts and N. benthamiana leaves and the protein appeared to bind viral DNA and ds/ssDNA nonspecifically, displaying novel DNA binding properties. Conclusions: Our results suggest that C4 protein in BSCTV is involved in symptom production and may facilitate virus movement instead of virus replication

Heat shock protein 27 is a potential indicator for response to YangZheng XiaoJi and chemotherapy agents in cancer cells

Heat shock protein 27 (HSP27) is a member of the heat shock protein family which has been linked to tumour progression and, most interestingly, to chemotherapy resistance in cancer patients. The present study examined the potential interplay between HSP27 and YangZheng XiaoJi, a traditional Chinese medicine used in cancer treatment. A range of cell lines from different tumour types including pancreatic, lung, gastric, colorectal, breast, prostate and ovarian cancer (both wild-type and resistant) were used. Levels and activation of HSP27 and its potential associated signalling pathways were evaluated by protein array and western blotting. Knockdown of HSP27 in cancer cells was achieved using siRNA. Localisation and co-localisation of HSP27 and other proteins were carried out by immunofluorescence. Cell growth and migration were evaluated in their response to a range of chemotherapeutic agents. The present study first identified, by way of protein array, that YangZheng XiaoJi was able to inhibit the phosphorylation of HSP27 protein in cancer cells. We further demonstrated that HSP27, which is co-localised with caspase-9, can be blocked from localising in focal adhesions and co-localising with caspase-9 by YangZheng XiaoJi. The study also demonstrated that YangZheng XiaoJi was able to sensitise cancer cells including those cells that were resistant to chemotherapy, to chemotherapeutic agents. Finally, knocking down HSP27 markedly reduced the migration of cancer cells and increased the sensitivity of cancer cells to the inhibitory effect on cellular migration by YangZheng XiaoJi. YangZheng XiaoJi can act as an agent in first sensitising cancer cells to chemotherapy and secondly to overcome, to some degree, chemoresistance when used in an appropriate fashion in patients who have active HSP2

The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer

The downstream of tyrosine kinase 7 (DOK7) is an adaptor protein mediating signalling transduction between receptors and intracellular downstream molecules. Reduced expression of DOK7 has been observed in breast cancer. The present study aimed to investigate the role played by DOK7 in lung cancer. The expression of DOK7 at both mRNA and protein levels was evaluated in human lung cancer. A reduced expression of DOK7 transcripts was seen in lung cancers compared with normal lung tissues. Kaplan-Meier analyses showed that the reduced expression of DOK7 was associated with poorer overall survival and progression-free survival of patients with lung cancer. A further western blot analysis revealed a predominant expression of DOK7 isoform 1 (DOK7V1) in normal lung tissues, which was reduced in lung cancer. Forced overexpression of DOK7V1 in lung cancer cell lines, A549 and H3122 resulted in a decrease of in vitro cell proliferation and migration, while adhesion to extracellular matrix was enhanced following the expression. In conclusion, DOK7 was reduced in lung cancer and reduced DOK7 expression was associated with poorer survival. DOK7 isoform 1 plays an inhibitory role on the proliferation and migration of lung cancer cells in which Akt pathway may be involved

Spatial and temporal distribution characteristics and prediction analysis of nitrogen and phosphorus surface source pollution in Shandong Province under the climate and land use changes

The aim of this study was to explore the characteristics of non-point source pollution of nitrogen (N) and phosphorus (P) under the background of climate and land use in Shandong Province. First, using the InVEST NDR module in the model, N and P non-point source pollution in 2010 and 2020 in Shandong Province were simulated; then, based on precipitation data under three different global climate models (MRI-ESM-0, GFDL-ESM4, and Ec-Earth3) and two Shared Socioeconomic Pathways (SSP245 and SSP585), land use data under two Shared Socioeconomic Pathways (SSP245 and SSP585) were used to simulate and predict the non-point source pollution of N and P in Shandong Province in 2030. The results showed the following: (1) On the time scale, the output load and total output of N and P decreased during 2010–2020, while the output load and total output of N and P increased during 2020–2030. (2) On the spatial scale, the spatial distribution of N and P output loads in 2010, 2020, and 2030 is roughly the same, being “low in the northwest and high in the southeast”. (3) Different climate scenarios have a great influence on N and P output load and total output, and the N and P pollution in the SSP585 scenario is more serious. The total output of N and P did not change much in different climate models, while the spatial distribution of the output load of N and P varied significantly, indicating that different climate models had a greater impact on the spatial distribution of the output load of N and P. (4) The overall cold hot spot pattern of nitrogen and phosphorus pollution in Shandong Province is stable, basically showing a “band + cluster + scatter” distribution pattern; the hot spot area in the central and southern region of Shandong Province changes little regardless of the model, the northwest is basically a cold spot area, and the nitrogen and phosphorus hot spot area under the SSP245 scenario in Ec-Earth3 model had the least amount of change. According to research results, combined with the actual situation of Shandong Province, it is hoped that it can provide theoretical basis for the prevention and control of non-point source pollution in Shandong Province in the future